ID   PEX12_PICPA             Reviewed;         409 AA.
AC   Q01961;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Peroxisome assembly protein 12;
DE   AltName: Full=Peroxin-12;
DE   AltName: Full=Peroxisome assembly protein PAS10;
GN   Name=PEX12; Synonyms=PAS10;
OS   Komagataella pastoris (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=4922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8670828; DOI=10.1002/j.1460-2075.1996.tb00692.x;
RA   Kalish J.E., Keller G.-A., Morrell J.C., Mihalik S.J., Smith B.,
RA   Cregg J.M., Gould S.J.;
RT   "Characterization of a novel component of the peroxisomal protein import
RT   apparatus using fluorescent peroxisomal proteins.";
RL   EMBO J. 15:3275-3285(1996).
CC   -!- FUNCTION: Required for protein import into peroxisomes.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the pex2/pex10/pex12 family. {ECO:0000305}.
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DR   EMBL; U58140; AAC49402.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q01961; -.
DR   GO; GO:0005779; C:integral component of peroxisomal membrane; IEA:InterPro.
DR   GO; GO:0008022; F:protein C-terminus binding; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0016558; P:protein import into peroxisome matrix; IEA:InterPro.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR017375; PEX12.
DR   InterPro; IPR006845; Pex_N.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR12888; PTHR12888; 1.
DR   Pfam; PF04757; Pex2_Pex12; 1.
DR   PIRSF; PIRSF038074; Peroxisome_assembly_p12; 1.
DR   SMART; SM00184; RING; 1.
PE   3: Inferred from homology;
KW   Membrane; Metal-binding; Peroxisome; Protein transport; Transmembrane;
KW   Transmembrane helix; Transport; Zinc; Zinc-finger.
FT   CHAIN           1..409
FT                   /note="Peroxisome assembly protein 12"
FT                   /id="PRO_0000218616"
FT   TRANSMEM        171..191
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        267..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ZN_FING         339..384
FT                   /note="RING-type; degenerate"
SQ   SEQUENCE   409 AA;  47591 MW;  1F2DB79A957C3B7F CRC64;
     MDFYSNLDSR SLDSETPTLF EIISAQELEK LLTPSIRYIL VHYTQRYPRY LLKVANHFDE
     LNLAIRGFIE FRQLSHWNST FIDKFYGLKK VRNHQTISTE RLQSQVPTLL EQRRRLSKTQ
     IAVSLFEIVG VPYLRDKLDH LYDKLYPKLM MNNLDPKESL KTFVQYYFLK LYPILLSVLT
     TIQVLLQVLY LSGTFKSPSI IMWLFKMKYA RLNSYDYTLD EQRVNKFLNK TSPGKLGTGN
     NRIRPITLTE SLYLLYSDLT RPLKKGLLIT GGTLFPASIF LLKFLEWWNS SDFATKMNKP
     RNPFSDSELP PPINLSKDLL ADRKIKKLLK KSQSNDGTCP LCHKQITNPA VIETGYVFCY
     TCIFKHLTSS ELDEETGGRC PITGRRLLGC RINKTTGEWT VDGIRRLMM