PEX12_YEAST
ID PEX12_YEAST Reviewed; 399 AA.
AC Q04370; D6VZK0; Q6B1L4;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Peroxisome assembly protein 12;
DE AltName: Full=Peroxin-12;
GN Name=PEX12; OrderedLocusNames=YMR026C; ORFNames=YM9711.16C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for protein import into peroxisomes. {ECO:0000250}.
CC -!- INTERACTION:
CC Q04370; Q05568: PEX10; NbExp=12; IntAct=EBI-2077297, EBI-13194;
CC Q04370; P80667: PEX13; NbExp=6; IntAct=EBI-2077297, EBI-13206;
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 907 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the pex2/pex10/pex12 family. {ECO:0000305}.
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DR EMBL; Z49211; CAA89129.1; -; Genomic_DNA.
DR EMBL; AY693066; AAT93085.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09924.1; -; Genomic_DNA.
DR PIR; S54028; S54028.
DR RefSeq; NP_013739.1; NM_001182522.1.
DR AlphaFoldDB; Q04370; -.
DR BioGRID; 35198; 248.
DR ComplexPortal; CPX-1903; Perxosomal PEX2-PEX10-PEX12 ubiquitin ligase complex.
DR DIP; DIP-7940N; -.
DR IntAct; Q04370; 11.
DR MINT; Q04370; -.
DR STRING; 4932.YMR026C; -.
DR TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR MaxQB; Q04370; -.
DR PaxDb; Q04370; -.
DR PRIDE; Q04370; -.
DR EnsemblFungi; YMR026C_mRNA; YMR026C; YMR026C.
DR GeneID; 855041; -.
DR KEGG; sce:YMR026C; -.
DR SGD; S000004628; PEX12.
DR VEuPathDB; FungiDB:YMR026C; -.
DR eggNOG; KOG0826; Eukaryota.
DR GeneTree; ENSGT00390000016209; -.
DR HOGENOM; CLU_031067_0_0_1; -.
DR InParanoid; Q04370; -.
DR OMA; VYCWKCI; -.
DR BioCyc; YEAST:G3O-32731-MON; -.
DR Reactome; R-SCE-8866654; E3 ubiquitin ligases ubiquitinate target proteins.
DR Reactome; R-SCE-9033241; Peroxisomal protein import.
DR Reactome; R-SCE-9603798; Class I peroxisomal membrane protein import.
DR PRO; PR:Q04370; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04370; protein.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; IDA:SGD.
DR GO; GO:1990429; C:peroxisomal importomer complex; IDA:SGD.
DR GO; GO:0000151; C:ubiquitin ligase complex; IPI:ComplexPortal.
DR GO; GO:0008022; F:protein C-terminus binding; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:SGD.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016558; P:protein import into peroxisome matrix; IMP:SGD.
DR GO; GO:0006513; P:protein monoubiquitination; IDA:SGD.
DR GO; GO:0016567; P:protein ubiquitination; IDA:ComplexPortal.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR017375; PEX12.
DR InterPro; IPR006845; Pex_N.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR12888; PTHR12888; 1.
DR Pfam; PF04757; Pex2_Pex12; 1.
DR PIRSF; PIRSF038074; Peroxisome_assembly_p12; 1.
PE 1: Evidence at protein level;
KW Membrane; Metal-binding; Peroxisome; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Zinc; Zinc-finger.
FT CHAIN 1..399
FT /note="Peroxisome assembly protein 12"
FT /id="PRO_0000218617"
FT TRANSMEM 270..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ZN_FING 334..373
FT /note="RING-type; degenerate"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 37
FT /note="I -> T (in Ref. 3; AAT93085)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 45992 MW; 98E6300C2CBFA16A CRC64;
MSFYSNLPSA GQSSRGSSTS GRNGVGLEPL YPTIFEIMSS QEIDSLLPAS IRYLLANHLV
ANFPNRYTLR LNKYFFEWFQ AIKGFVEWYH LKTYNSTFID RFYGLQLFSS RDRNLALTQC
LNPKGQSEWP QGLQLNQQQK SVIFLEKIIL PYITAKLDEI LEKISMNNIF SSDETENKWP
KRAFLRIYPF IKKLLALSNL LVKLLFLTKR TGSVSLLQYL FKIEYTTVRP LSSELSGLKE
TKGMDNRLRK TNISSIFALM QGQLSIIPRF LTFMGSQFFP TFIFVLRVYQ WWTTQDMTTK
LQKRVNDLDE DIPRPPFSSH SDKTEDKEGV SEACPVCEKT VQNPCVLETG YVACYPCAIS
YLVNNEGHCP VTNKKLLGCT YNKHTNKWEV VTGIRKLLI
