ID   PEX13_PICPA             Reviewed;         380 AA.
AC   Q92266;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Peroxisomal membrane protein PEX13;
DE   AltName: Full=Peroxin-13;
GN   Name=PEX13;
OS   Komagataella pastoris (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=4922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS.
RX   PubMed=8858165; DOI=10.1083/jcb.135.1.85;
RA   Gould S.J., Kalish J.E., Morrell J.C., Bjoerkman J., Urquhart A.J.,
RA   Crane D.I.;
RT   "Pex13p is an SH3 protein of the peroxisome membrane and a docking factor
RT   for the predominantly cytoplasmic PTs1 receptor.";
RL   J. Cell Biol. 135:85-95(1996).
CC   -!- FUNCTION: Component of the peroxisomal translocation machinery with
CC       PEX14 and PEX17. Interacts with the PTS1 receptor (PAS10/PEX5).
CC       Involved in the import of PTS1 and PTS2 proteins.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane; Single-pass membrane
CC       protein.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=PEX13L;
CC         IsoId=Q92266-1; Sequence=Displayed;
CC       Name=PEX13S;
CC         IsoId=Q92266-2; Sequence=VSP_018788;
CC   -!- SIMILARITY: Belongs to the peroxin-13 family. {ECO:0000305}.
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DR   EMBL; U70067; AAB09087.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q92266; -.
DR   SMR; Q92266; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005778; C:peroxisomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016560; P:protein import into peroxisome matrix, docking; IEA:InterPro.
DR   InterPro; IPR007223; Peroxin-13_N.
DR   InterPro; IPR035463; Pex13.
DR   InterPro; IPR036028; SH3-like_dom_sf.
DR   InterPro; IPR001452; SH3_domain.
DR   PANTHER; PTHR19332; PTHR19332; 1.
DR   Pfam; PF04088; Peroxin-13_N; 1.
DR   Pfam; PF14604; SH3_9; 1.
DR   PRINTS; PR00452; SH3DOMAIN.
DR   SMART; SM00326; SH3; 1.
DR   SUPFAM; SSF50044; SSF50044; 1.
DR   PROSITE; PS50002; SH3; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Membrane; Peroxisome; Protein transport;
KW   SH3 domain; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..380
FT                   /note="Peroxisomal membrane protein PEX13"
FT                   /id="PRO_0000022039"
FT   TOPO_DOM        1..230
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        231..251
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        252..380
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          277..344
FT                   /note="SH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00192"
FT   REGION          1..30
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..32
FT                   /note="Missing (in isoform PEX13S)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_018788"
FT   MUTAGEN         286
FT                   /note="Y->A: No effect on activity."
FT                   /evidence="ECO:0000269|PubMed:8858165"
FT   MUTAGEN         287
FT                   /note="D->A: No effect on activity."
FT                   /evidence="ECO:0000269|PubMed:8858165"
FT   MUTAGEN         288
FT                   /note="F->A: No effect on activity."
FT                   /evidence="ECO:0000269|PubMed:8858165"
FT   MUTAGEN         291
FT                   /note="E->K: Abolishes activity."
FT                   /evidence="ECO:0000269|PubMed:8858165"
FT   MUTAGEN         296
FT                   /note="E->K: Abolishes activity."
FT                   /evidence="ECO:0000269|PubMed:8858165"
SQ   SEQUENCE   380 AA;  40695 MW;  EEBABC39F93BA832 CRC64;
     MSDSSAPDLP SKPSSLNAGQ SSSLQTTNTG IGMGSGMGSG MGMGTYGNSY GSSYGGGYGS
     SMYGSGGYGM GGYGSSMYGG SRYGMGSYGM GGYGMGGYGM GMNTGMNGMG MAGSLAQGSE
     ATFQLIESII GAVGGFAQVL EATYMATHSS FFTMISMADQ LSHLKTALGS MLGIYTVINW
     LKRIMGKLMG VKNKLTPDEF RKFQEKQMKK LSNSNNTGGP NKNTNKLSLK PLLLFLAAVV
     GFPYLLKKLI AHLAETSQMN GNFITSGGSL QGNLDPTKLE FARALYDFNP ENEEMELKLA
     RGELMAILSK TEPNSNQEST WWKCRSRDGK VGFVPYNYVE IIERHQRPVP EAQEEPAAAV
     LAERQQQPII DSTEFQKMKT