PEX18_YEAST
ID PEX18_YEAST Reviewed; 283 AA.
AC P38855; D3DLA9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Peroxisomal membrane protein PEX18;
DE AltName: Full=Peroxin-18;
GN Name=PEX18; OrderedLocusNames=YHR160C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH PEX7.
RX PubMed=9864360; DOI=10.1083/jcb.143.7.1859;
RA Purdue P.E., Yang X., Lazarow P.B.;
RT "Pex18p and Pex21p, a novel pair of related peroxins essential for
RT peroxisomal targeting by the PTS2 pathway.";
RL J. Cell Biol. 143:1859-1869(1998).
RN [4]
RP UBIQUITINATION, INTERACTION WITH PEX7, AND STABILITY.
RX PubMed=11590152; DOI=10.1074/jbc.m106823200;
RA Purdue P.E., Lazarow P.B.;
RT "Pex18p is constitutively degraded during peroxisome biogenesis.";
RL J. Biol. Chem. 276:47684-47689(2001).
RN [5]
RP FUNCTION, AND INTERACTION WITH PEX7 AND PEX13.
RX PubMed=12167700; DOI=10.1128/mcb.22.17.6056-6069.2002;
RA Stein K., Schell-Steven A., Erdmann R., Rottensteiner H.;
RT "Interactions of Pex7p and Pex18p/Pex21p with the peroxisomal docking
RT machinery: implications for the first steps in PTS2 protein import.";
RL Mol. Cell. Biol. 22:6056-6069(2002).
RN [6]
RP FUNCTION.
RX PubMed=15456864; DOI=10.1128/mcb.24.20.8895-8906.2004;
RA Schaefer A., Kerssen D., Veenhuis M., Kunau W.-H., Schliebs W.;
RT "Functional similarity between the peroxisomal PTS2 receptor binding
RT protein Pex18p and the N-terminal half of the PTS1 receptor Pex5p.";
RL Mol. Cell. Biol. 24:8895-8906(2004).
CC -!- FUNCTION: Involved in peroxisome biogenesis and the import of
CC peroxisomal matrix proteins that contain the peroxisomal targeting
CC sequence PTS2. Required for peroxisomal targeting of PEX7 and growth on
CC oleate. {ECO:0000269|PubMed:12167700, ECO:0000269|PubMed:15456864,
CC ECO:0000269|PubMed:9864360}.
CC -!- SUBUNIT: Interacts with PEX7 and PEX13. The interaction with PEX7
CC stabilizes PEX18. {ECO:0000269|PubMed:11590152,
CC ECO:0000269|PubMed:12167700, ECO:0000269|PubMed:9864360}.
CC -!- INTERACTION:
CC P38855; P39108: PEX7; NbExp=6; IntAct=EBI-24803, EBI-13183;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9864360}.
CC Peroxisome membrane {ECO:0000269|PubMed:9864360}; Peripheral membrane
CC protein {ECO:0000269|PubMed:9864360}; Cytoplasmic side
CC {ECO:0000269|PubMed:9864360}.
CC -!- PTM: Ubiquitinated in a UBC4/UBC5 dependent manner.
CC {ECO:0000269|PubMed:11590152}.
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DR EMBL; U10397; AAB68992.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06853.1; -; Genomic_DNA.
DR PIR; S46770; S46770.
DR RefSeq; NP_012030.1; NM_001179291.1.
DR AlphaFoldDB; P38855; -.
DR SMR; P38855; -.
DR BioGRID; 36594; 78.
DR ComplexPortal; CPX-1905; Peroxisomal PEX7-PEX18 receptor complex.
DR DIP; DIP-1502N; -.
DR IntAct; P38855; 5.
DR MINT; P38855; -.
DR STRING; 4932.YHR160C; -.
DR TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR iPTMnet; P38855; -.
DR PaxDb; P38855; -.
DR PRIDE; P38855; -.
DR EnsemblFungi; YHR160C_mRNA; YHR160C; YHR160C.
DR GeneID; 856565; -.
DR KEGG; sce:YHR160C; -.
DR SGD; S000001203; PEX18.
DR VEuPathDB; FungiDB:YHR160C; -.
DR HOGENOM; CLU_984200_0_0_1; -.
DR OMA; PINSHID; -.
DR BioCyc; YEAST:G3O-31195-MON; -.
DR PRO; PR:P38855; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38855; protein.
DR GO; GO:0062137; C:cargo receptor complex; IPI:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IDA:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005778; C:peroxisomal membrane; IDA:ComplexPortal.
DR GO; GO:0005777; C:peroxisome; IDA:SGD.
DR GO; GO:0016558; P:protein import into peroxisome matrix; IMP:SGD.
PE 1: Evidence at protein level;
KW Cytoplasm; Membrane; Peroxisome; Reference proteome; Ubl conjugation.
FT CHAIN 1..283
FT /note="Peroxisomal membrane protein PEX18"
FT /id="PRO_0000202929"
FT REGION 179..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 283 AA; 32037 MW; 574C644FD7ED7A71 CRC64;
MNSNRCQTNE VNKFISSTEK GPFTGRDNTL SFNKIGSRLN SPPILKDKIE LKFLQHSEDL
NQSRSYVNIR PRTLEDQSYK FEAPNLNDNE TSWAKDFRYN FPKNVEPPIE NQIANLNINN
GLRTSQTDFP LGFYSQKNFN IASFPVVDHQ IFKTTGLEHP INSHIDSLIN AEFSELEASS
LEEDVHTEEE NSGTSLEDEE TAMKGLASDI IEFCDNNSAN KDVKERLNSS KFMGLMGSIS
DGSIVLKKDN GTERNLQKHV GFCFQNSGNW AGLEFHDVED RIA
