PEX22_YEAST
ID PEX22_YEAST Reviewed; 180 AA.
AC P39718; D6VPG3;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Peroxisome assembly protein 22;
DE AltName: Full=Peroxin-22;
GN Name=PEX22; OrderedLocusNames=YAL055W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA Storms R.K.;
RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION AS PEX22.
RX PubMed=10402463; DOI=10.1083/jcb.146.1.99;
RA Koller A., Snyder W.B., Faber K.N., Wenzel T.J., Rangell L., Keller G.A.,
RA Subramani S.;
RT "Pex22p of Pichia pastoris, essential for peroxisomal matrix protein
RT import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal
RT membrane.";
RL J. Cell Biol. 146:99-112(1999).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Involved in peroxisome biogenesis. {ECO:0000250}.
CC -!- INTERACTION:
CC P39718; P29340: PEX4; NbExp=10; IntAct=EBI-20707, EBI-19784;
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane {ECO:0000250}; Single-pass
CC membrane protein {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 259 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the peroxin-22 family. {ECO:0000305}.
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DR EMBL; U12980; AAC04978.1; -; Genomic_DNA.
DR EMBL; AY558167; AAS56493.1; -; Genomic_DNA.
DR EMBL; BK006935; DAA06933.1; -; Genomic_DNA.
DR PIR; S51966; S51966.
DR RefSeq; NP_009346.1; NM_001178198.1.
DR PDB; 2Y9M; X-ray; 2.60 A; B=54-180.
DR PDB; 2Y9P; X-ray; 3.25 A; B=54-180.
DR PDB; 4BWF; X-ray; 3.23 A; B=54-180.
DR PDBsum; 2Y9M; -.
DR PDBsum; 2Y9P; -.
DR PDBsum; 4BWF; -.
DR AlphaFoldDB; P39718; -.
DR SMR; P39718; -.
DR BioGRID; 31774; 214.
DR DIP; DIP-2791N; -.
DR IntAct; P39718; 4.
DR MINT; P39718; -.
DR STRING; 4932.YAL055W; -.
DR TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR MaxQB; P39718; -.
DR PaxDb; P39718; -.
DR PRIDE; P39718; -.
DR EnsemblFungi; YAL055W_mRNA; YAL055W; YAL055W.
DR GeneID; 851244; -.
DR KEGG; sce:YAL055W; -.
DR SGD; S000000051; PEX22.
DR VEuPathDB; FungiDB:YAL055W; -.
DR eggNOG; ENOG502SFA5; Eukaryota.
DR HOGENOM; CLU_121063_0_0_1; -.
DR InParanoid; P39718; -.
DR OMA; MWACVKS; -.
DR BioCyc; YEAST:G3O-28859-MON; -.
DR PRO; PR:P39718; -.
DR Proteomes; UP000002311; Chromosome I.
DR RNAct; P39718; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005778; C:peroxisomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0097027; F:ubiquitin-protein transferase activator activity; IDA:SGD.
DR GO; GO:1902499; P:positive regulation of protein autoubiquitination; IDA:SGD.
DR GO; GO:0031398; P:positive regulation of protein ubiquitination; IDA:SGD.
DR GO; GO:0016562; P:protein import into peroxisome matrix, receptor recycling; IMP:SGD.
DR Gene3D; 3.40.50.11730; -; 1.
DR InterPro; IPR024359; Peroxin-22.
DR InterPro; IPR038613; Peroxin-22_C_sf.
DR Pfam; PF12827; Peroxin-22; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Membrane; Peroxisome; Peroxisome biogenesis;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..180
FT /note="Peroxisome assembly protein 22"
FT /id="PRO_0000058339"
FT TRANSMEM 15..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT STRAND 62..65
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 77..79
FT /evidence="ECO:0007829|PDB:2Y9M"
FT TURN 80..82
FT /evidence="ECO:0007829|PDB:2Y9M"
FT STRAND 83..90
FT /evidence="ECO:0007829|PDB:2Y9M"
FT TURN 91..93
FT /evidence="ECO:0007829|PDB:2Y9M"
FT STRAND 94..97
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 100..107
FT /evidence="ECO:0007829|PDB:2Y9M"
FT TURN 108..110
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:2Y9M"
FT STRAND 115..119
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 121..131
FT /evidence="ECO:0007829|PDB:2Y9M"
FT STRAND 134..138
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 140..142
FT /evidence="ECO:0007829|PDB:2Y9M"
FT STRAND 145..148
FT /evidence="ECO:0007829|PDB:2Y9P"
FT HELIX 151..153
FT /evidence="ECO:0007829|PDB:2Y9P"
FT HELIX 158..160
FT /evidence="ECO:0007829|PDB:2Y9M"
FT STRAND 163..167
FT /evidence="ECO:0007829|PDB:2Y9M"
FT HELIX 171..179
FT /evidence="ECO:0007829|PDB:2Y9M"
SQ SEQUENCE 180 AA; 19877 MW; 2D2E34BB93F756EF CRC64;
MPPPSRSRIN KTRTLGIVGT AIAVLVTSYY IYQKVTSAKE DNGARPPEGD SVKENKKARK
SKCIIMSKSI QGLPIKWEEY AADEVVLLVP TSHTDGSMKQ AIGDAFRKTK NEHKIIYCDS
MDGLWSCVRR LGKFQCILNS RDFTSSGGSD AAVVPEDIGR FVKFVVDSDV EDVLIDTLCN
