ID   PEX2_CRIGR              Reviewed;         304 AA.
AC   Q06438;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Peroxisome biogenesis factor 2;
DE   AltName: Full=Peroxin-2;
DE   AltName: Full=Peroxisomal membrane protein 3;
DE   AltName: Full=Peroxisome assembly factor 1;
DE            Short=PAF-1;
GN   Name=PEX2; Synonyms=PAF1, PMP35, PXMP3;
OS   Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC   Cricetidae; Cricetinae; Cricetulus.
OX   NCBI_TaxID=10029;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Ovary;
RX   PubMed=7685346; DOI=10.1016/s0021-9258(18)31435-2;
RA   Thieringer R., Raetz C.R.H.;
RT   "Peroxisome-deficient Chinese hamster ovary cells with point mutations in
RT   peroxisome assembly factor-1.";
RL   J. Biol. Chem. 268:12631-12636(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8035823; DOI=10.1128/mcb.14.8.5458-5465.1994;
RA   Tsukamoto T., Shimozawa N., Fujiki Y.;
RT   "Peroxisome assembly factor 1: nonsense mutation in a peroxisome-deficient
RT   Chinese hamster ovary cell mutant and deletion analysis.";
RL   Mol. Cell. Biol. 14:5458-5465(1994).
CC   -!- FUNCTION: Somewhat implicated in the biogenesis of peroxisomes.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the pex2/pex10/pex12 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Z17220; CAA78929.1; -; mRNA.
DR   EMBL; D30618; BAA06308.1; -; mRNA.
DR   PIR; A45989; A45989.
DR   RefSeq; NP_001230964.1; NM_001244035.1.
DR   RefSeq; XP_007644647.1; XM_007646457.2.
DR   AlphaFoldDB; Q06438; -.
DR   STRING; 10029.NP_001230964.1; -.
DR   PRIDE; Q06438; -.
DR   Ensembl; ENSCGRT00001018576; ENSCGRP00001014339; ENSCGRG00001015243.
DR   GeneID; 100689047; -.
DR   KEGG; cge:100689047; -.
DR   CTD; 5828; -.
DR   eggNOG; KOG2879; Eukaryota.
DR   GeneTree; ENSGT00390000001846; -.
DR   OMA; SCFHGFK; -.
DR   GO; GO:0016593; C:Cdc73/Paf1 complex; IEA:Ensembl.
DR   GO; GO:0005779; C:integral component of peroxisomal membrane; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IEA:Ensembl.
DR   GO; GO:0050680; P:negative regulation of epithelial cell proliferation; IEA:Ensembl.
DR   GO; GO:0048147; P:negative regulation of fibroblast proliferation; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0007031; P:peroxisome organization; ISS:UniProtKB.
DR   GO; GO:0031648; P:protein destabilization; IEA:Ensembl.
DR   GO; GO:0016558; P:protein import into peroxisome matrix; IEA:Ensembl.
DR   GO; GO:0000038; P:very long-chain fatty acid metabolic process; IEA:Ensembl.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR006845; Pex_N.
DR   InterPro; IPR018957; Znf_C3HC4_RING-type.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   InterPro; IPR017907; Znf_RING_CS.
DR   Pfam; PF04757; Pex2_Pex12; 1.
DR   Pfam; PF00097; zf-C3HC4; 1.
DR   SMART; SM00184; RING; 1.
DR   PROSITE; PS00518; ZF_RING_1; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   1: Evidence at protein level;
KW   Membrane; Metal-binding; Peroxisome; Transmembrane; Transmembrane helix;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..304
FT                   /note="Peroxisome biogenesis factor 2"
FT                   /id="PRO_0000056368"
FT   TRANSMEM        139..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        194..212
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   ZN_FING         243..283
FT                   /note="RING-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT   MUTAGEN         246
FT                   /note="C->Y: Loss of function."
SQ   SEQUENCE   304 AA;  34795 MW;  84EC5FA613C148BF CRC64;
     MAGREKTKSA NRVLRISQLD ALELNKALEQ LVWSQFTQCF HGFKPGLLAR FEPEVKACLW
     LFLWRFTIYS KNATVGQSVL NIQYKNDFSS NSRYQPPSKN QKLWYAVCTI GGRWLEERCY
     DLFRNRHLAS FGKVKQCMNV MVGLLKLGEL INFLIFLQKG KFATLTERLL GIHSVFCKPQ
     NIREVGFDYM NRELLWHGFA EFLIFLLPLI NIQKFKAKLS SWCIPLTGAA SSDSALASSG
     KECALCGEWP TMPHTIGCEH VFCYYCVKSS FLFDMYFTCP KCGIEVHSVQ PLKSGIEMSE
     VNAL