PEX2_YEAST
ID PEX2_YEAST Reviewed; 271 AA.
AC P32800; D6VVY4;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Peroxisomal biogenesis factor 2;
DE AltName: Full=Peroxin-2;
DE AltName: Full=Peroxisomal protein PAS5;
DE AltName: Full=Protein CRT1;
GN Name=PEX2; Synonyms=CRT1, PAS5; OrderedLocusNames=YJL210W;
GN ORFNames=HRA271, J0240;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8686377;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<135::aid-yea892>3.0.co;2-w;
RA Liu Y., Gu K.L., Dieckmann C.L.;
RT "Independent regulation of full-length and 5'-truncated PAS5 mRNAs in
RT Saccharomyces cerevisiae.";
RL Yeast 12:135-143(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7725802; DOI=10.1002/yea.320101216;
RA Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F.;
RT "Sequence analysis of a 40.2 kb DNA fragment located near the left telomere
RT of yeast chromosome X.";
RL Yeast 10:1657-1662(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for the import of several proteins into peroxisomes.
CC -!- INTERACTION:
CC P32800; Q05568: PEX10; NbExp=14; IntAct=EBI-13160, EBI-13194;
CC P32800; P80667: PEX13; NbExp=10; IntAct=EBI-13160, EBI-13206;
CC P32800; P53112: PEX14; NbExp=10; IntAct=EBI-13160, EBI-13212;
CC -!- SUBCELLULAR LOCATION: Peroxisome.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=Long;
CC IsoId=P32800-1; Sequence=Displayed;
CC Name=Short;
CC IsoId=P32800-2; Sequence=VSP_018874;
CC -!- MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the pex2/pex10/pex12 family. {ECO:0000305}.
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DR EMBL; M86538; AAB36836.1; -; Genomic_DNA.
DR EMBL; Z34098; CAA84000.1; -; Genomic_DNA.
DR EMBL; Z49486; CAA89508.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08600.1; -; Genomic_DNA.
DR PIR; S27422; S27422.
DR RefSeq; NP_012325.1; NM_001181643.1. [P32800-1]
DR AlphaFoldDB; P32800; -.
DR BioGRID; 33548; 131.
DR ComplexPortal; CPX-1903; Perxosomal PEX2-PEX10-PEX12 ubiquitin ligase complex.
DR DIP; DIP-4760N; -.
DR IntAct; P32800; 9.
DR STRING; 4932.YJL210W; -.
DR TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR iPTMnet; P32800; -.
DR MaxQB; P32800; -.
DR PaxDb; P32800; -.
DR PRIDE; P32800; -.
DR EnsemblFungi; YJL210W_mRNA; YJL210W; YJL210W. [P32800-1]
DR GeneID; 853219; -.
DR KEGG; sce:YJL210W; -.
DR SGD; S000003746; PEX2.
DR VEuPathDB; FungiDB:YJL210W; -.
DR eggNOG; KOG2879; Eukaryota.
DR HOGENOM; CLU_1081737_0_0_1; -.
DR InParanoid; P32800; -.
DR OMA; YCYVCVL; -.
DR BioCyc; YEAST:G3O-31638-MON; -.
DR PRO; PR:P32800; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P32800; protein.
DR GO; GO:1990429; C:peroxisomal importomer complex; IDA:SGD.
DR GO; GO:0005778; C:peroxisomal membrane; IDA:SGD.
DR GO; GO:0000151; C:ubiquitin ligase complex; IPI:ComplexPortal.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IDA:SGD.
DR GO; GO:0016558; P:protein import into peroxisome matrix; IMP:SGD.
DR GO; GO:0000209; P:protein polyubiquitination; IDA:FlyBase.
DR GO; GO:0016567; P:protein ubiquitination; IDA:ComplexPortal.
DR InterPro; IPR006845; Pex_N.
DR Pfam; PF04757; Pex2_Pex12; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Metal-binding; Peroxisome; Reference proteome;
KW Zinc; Zinc-finger.
FT CHAIN 1..271
FT /note="Peroxisomal biogenesis factor 2"
FT /id="PRO_0000030724"
FT ZN_FING 222..259
FT /note="RING-type"
FT VAR_SEQ 1..46
FT /note="Missing (in isoform Short)"
FT /evidence="ECO:0000305"
FT /id="VSP_018874"
SQ SEQUENCE 271 AA; 30752 MW; FAEFA3A526E82F3E CRC64;
MSRVAQLDSI ALDKELYGQF WSEFNAAFNT SEHKEEWELA LNTVVFMCAT RFLPHYGSSC
TYGSALSGVV FQCRKRTLYV VTVLAGYVWK KITHIIFNGP HCGNQMMWLK LYKWVNLLYH
GCDVTNFLRF LAAEGPNARA FLSPLYRAFN VHSTRLIRDG SAIASEFYSN SVFAGLEYQN
RQLLWNALLE LFSNTLLTKR GLLTFVKKPP RSRSTTTYKT VCPRCGGFPT NPYQIACCRA
NYCYVCVVKA LEWSMCDACG SSGRLTASPV Y
