PEX32_YEAST
ID PEX32_YEAST Reviewed; 413 AA.
AC P38292; D6VQG4; E9P8W7;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Peroxisomal membrane protein PEX32;
DE AltName: Full=Peroxin-32;
GN Name=PEX32; OrderedLocusNames=YBR168W; ORFNames=YBR1220;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane
CC {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the PEX28-32 family. PEX32 subfamily.
CC {ECO:0000305}.
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DR EMBL; Z36037; CAA85129.1; -; Genomic_DNA.
DR EMBL; AY692688; AAT92707.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07284.1; -; Genomic_DNA.
DR PIR; S46039; S46039.
DR RefSeq; NP_009727.3; NM_001178516.3.
DR AlphaFoldDB; P38292; -.
DR SMR; P38292; -.
DR BioGRID; 32868; 198.
DR DIP; DIP-5361N; -.
DR IntAct; P38292; 1.
DR MINT; P38292; -.
DR STRING; 4932.YBR168W; -.
DR TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR MaxQB; P38292; -.
DR PaxDb; P38292; -.
DR PRIDE; P38292; -.
DR EnsemblFungi; YBR168W_mRNA; YBR168W; YBR168W.
DR GeneID; 852466; -.
DR KEGG; sce:YBR168W; -.
DR SGD; S000000372; PEX32.
DR VEuPathDB; FungiDB:YBR168W; -.
DR eggNOG; ENOG502QU0V; Eukaryota.
DR GeneTree; ENSGT00940000176349; -.
DR HOGENOM; CLU_665907_0_0_1; -.
DR InParanoid; P38292; -.
DR OMA; TDWIYSD; -.
DR BioCyc; YEAST:G3O-29116-MON; -.
DR PRO; PR:P38292; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38292; protein.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; IDA:SGD.
DR GO; GO:0007031; P:peroxisome organization; IMP:SGD.
DR InterPro; IPR010482; Peroxin.
DR InterPro; IPR006614; Peroxin/Ferlin.
DR InterPro; IPR029953; Pex32.
DR PANTHER; PTHR31679:SF3; PTHR31679:SF3; 1.
DR Pfam; PF06398; Pex24p; 1.
DR SMART; SM00694; DysFC; 1.
DR SMART; SM00693; DysFN; 1.
PE 1: Evidence at protein level;
KW Membrane; Peroxisome; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..413
FT /note="Peroxisomal membrane protein PEX32"
FT /id="PRO_0000202502"
FT TOPO_DOM 1..66
FT /note="Peroxisomal"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..181
FT /note="Peroxisomal"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..413
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 165
FT /note="S -> P (in Ref. 3; AAT92707)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 413 AA; 48579 MW; 11671920832A91AD CRC64;
MDTNSKTKVQ TENKKIKAKF IHNHGQKPSL IQITPPMISS TLFHAYPLLL IFDNALANIM
WLSDDKCLTF IYLTSIWLTI SFFIPVETEA SHFLPFTKIL RLWLGIISGA FLFLSFMYYI
VSLIASLRDT EPPTLDEIVV LLESVLDKLE VLRNELNVWK KLKLSFDGVN KECSGKRLFC
RLFLFGTIFQ IIIMRYISPG TYTRFFIITG LIYNTSSFQA TLRLLWRFTA VRNFYYLGIE
SFKISSFLPK HLKMEQIIPL SQGRAITVPL VEVLPKLLRD KKGDDHIHIL QLLLNEQKDN
FGNEDLKILE IEVYENQRRW YQNKNWSTKL LPYERQNYCI EIKNTDGTLT MRSCLPPDGL
GEEELPNNWH WINDNWDGTD WIYSDSAWKE IGQYSSLESF TRSRKWKRRL FHL
