PEX3_MOUSE
ID PEX3_MOUSE Reviewed; 372 AA.
AC Q9QXY9;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Peroxisomal biogenesis factor 3;
DE AltName: Full=Peroxin-3;
DE AltName: Full=Peroxisomal assembly protein PEX3;
GN Name=Pex3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=129/Ola;
RX PubMed=10839463; DOI=10.1515/bc.2000.044;
RA Muntau A.C., Mayerhofer P.U., Albet S., Schmid T.E., Bugaut M.,
RA Roscher A.A., Kammerer S.;
RT "Genomic organization, expression analysis, and chromosomal localization of
RT the mouse PEX3 gene encoding a peroxisomal assembly protein.";
RL Biol. Chem. 381:337-342(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland, and Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in peroxisome biosynthesis and integrity. Assembles
CC membrane vesicles before the matrix proteins are translocated. As a
CC docking factor for PEX19, is necessary for the import of peroxisomal
CC membrane proteins in the peroxisomes. {ECO:0000250|UniProtKB:P56589}.
CC -!- SUBUNIT: Interacts with PEX19. {ECO:0000250|UniProtKB:P56589}.
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane
CC {ECO:0000250|UniProtKB:P56589}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Identified in all tissues analyzed, with the
CC strongest expression in liver and in testis.
CC -!- SIMILARITY: Belongs to the peroxin-3 family. {ECO:0000305}.
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DR EMBL; AF152996; AAF14524.1; -; mRNA.
DR EMBL; AF162896; AAG24507.1; -; Genomic_DNA.
DR EMBL; AF162890; AAG24507.1; JOINED; Genomic_DNA.
DR EMBL; AF162891; AAG24507.1; JOINED; Genomic_DNA.
DR EMBL; AF162892; AAG24507.1; JOINED; Genomic_DNA.
DR EMBL; AF162893; AAG24507.1; JOINED; Genomic_DNA.
DR EMBL; AF162894; AAG24507.1; JOINED; Genomic_DNA.
DR EMBL; AF162895; AAG24507.1; JOINED; Genomic_DNA.
DR EMBL; BC033415; AAH33415.1; -; mRNA.
DR EMBL; BC037606; AAH37606.1; -; mRNA.
DR CCDS; CCDS23702.1; -.
DR RefSeq; NP_064345.1; NM_019961.3.
DR AlphaFoldDB; Q9QXY9; -.
DR SMR; Q9QXY9; -.
DR BioGRID; 208045; 1.
DR STRING; 10090.ENSMUSP00000019945; -.
DR iPTMnet; Q9QXY9; -.
DR PhosphoSitePlus; Q9QXY9; -.
DR SwissPalm; Q9QXY9; -.
DR EPD; Q9QXY9; -.
DR jPOST; Q9QXY9; -.
DR MaxQB; Q9QXY9; -.
DR PaxDb; Q9QXY9; -.
DR PRIDE; Q9QXY9; -.
DR ProteomicsDB; 301794; -.
DR Antibodypedia; 33142; 134 antibodies from 25 providers.
DR DNASU; 56535; -.
DR Ensembl; ENSMUST00000019945; ENSMUSP00000019945; ENSMUSG00000019809.
DR GeneID; 56535; -.
DR KEGG; mmu:56535; -.
DR UCSC; uc007eky.2; mouse.
DR CTD; 8504; -.
DR MGI; MGI:1929646; Pex3.
DR VEuPathDB; HostDB:ENSMUSG00000019809; -.
DR eggNOG; KOG4444; Eukaryota.
DR GeneTree; ENSGT00390000008481; -.
DR HOGENOM; CLU_041367_2_0_1; -.
DR InParanoid; Q9QXY9; -.
DR OMA; WSLQMAI; -.
DR OrthoDB; 891261at2759; -.
DR PhylomeDB; Q9QXY9; -.
DR TreeFam; TF352826; -.
DR Reactome; R-MMU-1369062; ABC transporters in lipid homeostasis.
DR Reactome; R-MMU-9603798; Class I peroxisomal membrane protein import.
DR BioGRID-ORCS; 56535; 11 hits in 73 CRISPR screens.
DR ChiTaRS; Pex3; mouse.
DR PRO; PR:Q9QXY9; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q9QXY9; protein.
DR Bgee; ENSMUSG00000019809; Expressed in spermatid and 248 other tissues.
DR ExpressionAtlas; Q9QXY9; baseline and differential.
DR Genevisible; Q9QXY9; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005778; C:peroxisomal membrane; ISO:MGI.
DR GO; GO:0005777; C:peroxisome; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0032994; C:protein-lipid complex; ISO:MGI.
DR GO; GO:0008289; F:lipid binding; ISO:MGI.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IBA:GO_Central.
DR GO; GO:0016557; P:peroxisome membrane biogenesis; ISO:MGI.
DR GO; GO:0007031; P:peroxisome organization; ISO:MGI.
DR GO; GO:0045046; P:protein import into peroxisome membrane; ISO:MGI.
DR InterPro; IPR006966; Peroxin-3.
DR PANTHER; PTHR28080; PTHR28080; 1.
DR Pfam; PF04882; Peroxin-3; 2.
PE 1: Evidence at protein level;
KW Membrane; Peroxisome; Peroxisome biogenesis; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..372
FT /note="Peroxisomal biogenesis factor 3"
FT /id="PRO_0000208739"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..116
FT /note="Peroxisomal"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..372
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..45
FT /note="Targeting to peroxisomes"
FT /evidence="ECO:0000250|UniProtKB:P56589"
FT REGION 120..136
FT /note="Interaction with PEX19"
FT /evidence="ECO:0000250|UniProtKB:P56589"
SQ SEQUENCE 372 AA; 42224 MW; 77AA9590A53FAB77 CRC64;
MLRSMWNFLK RHKKKCIFLG TVLGGVYILG KYGQKKIREI QEREAAEYIA QARRQYHFES
NQRTCNMTVL SMLPTLREAL MQQLNSESLT ALLKSRPSNK LEIWEDLKII SFTRSIVAVY
STCMLVVLLR VQLNIIGGYI YLDNATVGKN GTTVLAPPDV QQQYLSSIQH LLGDGLTELV
TVIKQAVQRI LGSVSLKHSL SLLDLEQKLK EIRILVEQHQ SSWNDKDVSR SSLCQYMMPD
EETPLAAQAY GLSHRDITTI KLLNETRDML ESPDFSTVLN TCLNRGFSRL LDNMAEFFRP
TEQDLQHGNS INSLSSVSLP LAKIIPIVNG QIHSVCSETP SHFVQDLLMM EQVKDFAANV
YEAFSTPQQL EK
