ID   PEX5_CAVPO              Reviewed;         640 AA.
AC   O70525;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Peroxisomal targeting signal 1 receptor;
DE            Short=PTS1 receptor;
DE            Short=PTS1R;
DE   AltName: Full=PTS1-BP;
DE   AltName: Full=Peroxin-5;
DE   AltName: Full=Peroxisomal C-terminal targeting signal import receptor;
DE   AltName: Full=Peroxisome receptor 1;
GN   Name=PEX5; Synonyms=PXR1;
OS   Cavia porcellus (Guinea pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC   Cavia.
OX   NCBI_TaxID=10141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Liver;
RX   PubMed=11085934; DOI=10.1042/bj3520409;
RA   Knott T.G., Birdsey G.M., Sinclair K.E., Gallagher I.M., Purdue P.E.,
RA   Danpure C.J.;
RT   "The peroxisomal targeting sequence type 1 receptor, Pex5p, and the
RT   peroxisomal import efficiency of alanine:glyoxylate aminotransferase.";
RL   Biochem. J. 352:409-418(2000).
CC   -!- FUNCTION: Binds to the C-terminal PTS1-type tripeptide peroxisomal
CC       targeting signal (SKL-type) and plays an essential role in peroxisomal
CC       protein import. {ECO:0000269|PubMed:11085934}.
CC   -!- SUBUNIT: Interacts with PEX7, PEX12, PEX13 and PEX14. Interacts (Cys-
CC       linked ubiquitinated) with ZFAND6 (By similarity). Interacts with VWA8
CC       in a PEX7-dependent manner (By similarity).
CC       {ECO:0000250|UniProtKB:P50542, ECO:0000250|UniProtKB:Q920N5}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q920N5}.
CC       Peroxisome membrane {ECO:0000250|UniProtKB:Q920N5}; Peripheral membrane
CC       protein {ECO:0000250|UniProtKB:Q920N5}. Note=Its distribution appears
CC       to be dynamic. It is probably a cycling receptor found mainly in the
CC       cytoplasm and as well associated to the peroxisomal membrane through a
CC       docking factor (PEX13) (By similarity). {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O70525-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O70525-2; Sequence=VSP_024715;
CC   -!- TISSUE SPECIFICITY: Detected in liver, kidney, adrenal gland, brain,
CC       adipose tissue, small intestine, heart, lung, muscle, ovary, spinal
CC       cord, skin and spleen. {ECO:0000269|PubMed:11085934}.
CC   -!- PTM: Monoubiquitination at Cys-11 is required for proper export from
CC       peroxisomes and recycling. {ECO:0000250|UniProtKB:Q920N5}.
CC   -!- SIMILARITY: Belongs to the peroxisomal targeting signal receptor
CC       family. {ECO:0000305}.
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DR   EMBL; AJ004953; CAA06241.1; -; mRNA.
DR   RefSeq; NP_001166469.1; NM_001172998.1. [O70525-1]
DR   AlphaFoldDB; O70525; -.
DR   SMR; O70525; -.
DR   STRING; 10141.ENSCPOP00000019431; -.
DR   GeneID; 100135597; -.
DR   KEGG; cpoc:100135597; -.
DR   CTD; 5830; -.
DR   eggNOG; KOG1125; Eukaryota.
DR   InParanoid; O70525; -.
DR   OrthoDB; 588648at2759; -.
DR   Proteomes; UP000005447; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0005782; C:peroxisomal matrix; ISS:UniProtKB.
DR   GO; GO:0005778; C:peroxisomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005777; C:peroxisome; ISS:UniProtKB.
DR   GO; GO:0005052; F:peroxisome matrix targeting signal-1 binding; ISS:UniProtKB.
DR   GO; GO:0016561; P:protein import into peroxisome matrix, translocation; ISS:UniProtKB.
DR   GO; GO:0006625; P:protein targeting to peroxisome; ISS:UniProtKB.
DR   GO; GO:0051262; P:protein tetramerization; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR024111; PEX5/PEX5L.
DR   InterPro; IPR024113; PEX5_animals.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR10130; PTHR10130; 1.
DR   PANTHER; PTHR10130:SF2; PTHR10130:SF2; 1.
DR   Pfam; PF13181; TPR_8; 1.
DR   SMART; SM00028; TPR; 5.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS50005; TPR; 5.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cytoplasm; Membrane; Peroxisome; Phosphoprotein;
KW   Protein transport; Reference proteome; Repeat; Thioester bond; TPR repeat;
KW   Transport; Ubl conjugation.
FT   CHAIN           1..640
FT                   /note="Peroxisomal targeting signal 1 receptor"
FT                   /id="PRO_0000284881"
FT   REPEAT          336..369
FT                   /note="TPR 1"
FT   REPEAT          371..403
FT                   /note="TPR 2"
FT   REPEAT          404..437
FT                   /note="TPR 3"
FT   REPEAT          454..486
FT                   /note="TPR 4"
FT   REPEAT          489..522
FT                   /note="TPR 5"
FT   REPEAT          524..556
FT                   /note="TPR 6"
FT   REPEAT          558..590
FT                   /note="TPR 7"
FT   MOD_RES         115
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P50542"
FT   MOD_RES         153
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P50542"
FT   MOD_RES         155
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P50542"
FT   MOD_RES         167
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P50542"
FT   MOD_RES         280
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P50542"
FT   CROSSLNK        11
FT                   /note="Glycyl cysteine thioester (Cys-Gly) (interchain with
FT                   G-Cter in ubiquitin)"
FT                   /evidence="ECO:0000250|UniProtKB:Q920N5"
FT   VAR_SEQ         216..252
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:11085934"
FT                   /id="VSP_024715"
SQ   SEQUENCE   640 AA;  70920 MW;  2C8A9F7F0F272176 CRC64;
     MAMRELVEGE CGGANPLMKL AGHFTQDKAL RQEGLRPGPW PPGAPASETV SKPLGVASED
     ELVAEFLQDQ NAPLVSRAPQ TFKMDDLLAE MQEIEQSNFR QAPQRAPGVA DLALSENWAQ
     EFLAAGDAVD VTQDYNETDW SQEFIAEVTD PLSVSPARWA EEYLEQSEEK LWLGEPEGAA
     TTDRWYDEYH PEEDLQHTAS DFVAKVDDPK LANSEFLKFV RQIGEGQVSL ESVAGSGRAQ
     AEQWAAEFIQ QQGTSDAWVD QFTRPVNTSA LDMEFERAKS AIESDVDFWD KLQAELEEMA
     KRDAEAHPWL SDYDDLTSAS YDKGYQFEEE NPLRDHPQPF EEGLLRLEEG DLPNAVLLFE
     AAVQQDPKHM EAWQYLGTTQ AENEQELLAI SALRRCLELK PDNRTALMAL AVSFTNESLQ
     RQACETLRDW LRCTPAYAHL VTPAEEGAGG AGLGSSKRIL GSLLSDSLFL EVKELFLAAV
     RLDPTSIDPD VQCGLGVLFN LSGEYDKAVD CFTAALSVRP NDYLLWNKLG ATLANGNQSE
     EAVAAYRRAL ELQPGYIRSR YNLGISCINL GAHREAVEHF LEALNMQRKS RGPRGEGGAM
     SENIWSTLRL ALSMLGQSDA YRAADARDLS ALLALFGLSQ