PEX6_RAT
ID PEX6_RAT Reviewed; 978 AA.
AC P54777; O55097;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Peroxisome assembly factor 2;
DE Short=PAF-2;
DE AltName: Full=Peroxin-6;
DE AltName: Full=Peroxisomal biogenesis factor 6;
DE AltName: Full=Peroxisomal-type ATPase 1;
GN Name=Pex6;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS OF LYS-476 AND LYS-748.
RC STRAIN=Fischer 344; TISSUE=Liver;
RX PubMed=7493019; DOI=10.1038/ng1295-395;
RA Tsukamoto T., Miura S., Nakai T., Yokota S., Shimozawa N., Suzuki Y.,
RA Orii T., Fujiki Y., Sakai F., Bogaki A., Yasumo H., Osumi T.;
RT "Peroxisome assembly factor-2, a putative ATPase cloned by functional
RT complementation on a peroxisome-deficient mammalian cell mutant.";
RL Nat. Genet. 11:395-401(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Sprague-Dawley;
RA Tsukamoto T., Hashiguchi N.;
RL Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in peroxisome biosynthesis. Required for stability
CC of the PTS1 receptor. Probably required for protein import into
CC peroxisomes. Anchored by PEX26 to peroxisome membranes, possibly to
CC form heteromeric AAA ATPase complexes required for the import of
CC proteins into peroxisomes (By similarity).
CC {ECO:0000250|UniProtKB:Q13608}.
CC -!- SUBUNIT: Interacts directly with PEX26 and PEX1. Mediates the indirect
CC interaction between PEX1 and PEX26. Interacts with ZFAND6 (By
CC similarity). {ECO:0000250|UniProtKB:Q13608}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Peroxisome membrane
CC {ECO:0000250|UniProtKB:Q13608}. Cell projection, cilium, photoreceptor
CC outer segment {ECO:0000250|UniProtKB:Q13608}. Note=Associated with
CC peroxisomal membranes. Localized at the base of the outer segment of
CC photoreceptor cells (By similarity). {ECO:0000250|UniProtKB:Q13608}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family. {ECO:0000305}.
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DR EMBL; D63673; BAA09824.1; -; mRNA.
DR EMBL; D89660; BAA24931.1; -; Genomic_DNA.
DR RefSeq; NP_476466.1; NM_057125.1.
DR AlphaFoldDB; P54777; -.
DR SMR; P54777; -.
DR BioGRID; 250712; 2.
DR STRING; 10116.ENSRNOP00000022582; -.
DR jPOST; P54777; -.
DR PaxDb; P54777; -.
DR PRIDE; P54777; -.
DR ABCD; P54777; 1 sequenced antibody.
DR Ensembl; ENSRNOT00000022582; ENSRNOP00000022582; ENSRNOG00000016655.
DR GeneID; 117265; -.
DR KEGG; rno:117265; -.
DR UCSC; RGD:621637; rat.
DR CTD; 5190; -.
DR RGD; 621637; Pex6.
DR eggNOG; KOG0736; Eukaryota.
DR GeneTree; ENSGT00550000074953; -.
DR HOGENOM; CLU_000688_0_8_1; -.
DR InParanoid; P54777; -.
DR OMA; ALQPCIL; -.
DR OrthoDB; 233419at2759; -.
DR PhylomeDB; P54777; -.
DR Reactome; R-RNO-9033241; Peroxisomal protein import.
DR PRO; PR:P54777; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000016655; Expressed in liver and 19 other tissues.
DR Genevisible; P54777; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0005778; C:peroxisomal membrane; IDA:HGNC-UCL.
DR GO; GO:0005777; C:peroxisome; ISO:RGD.
DR GO; GO:0097733; C:photoreceptor cell cilium; ISS:UniProtKB.
DR GO; GO:0001750; C:photoreceptor outer segment; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; ISO:RGD.
DR GO; GO:0016887; F:ATP hydrolysis activity; ISO:RGD.
DR GO; GO:0008022; F:protein C-terminus binding; ISO:RGD.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:RGD.
DR GO; GO:0007031; P:peroxisome organization; IMP:RGD.
DR GO; GO:0016558; P:protein import into peroxisome matrix; IBA:GO_Central.
DR GO; GO:0016561; P:protein import into peroxisome matrix, translocation; ISO:RGD.
DR GO; GO:0050821; P:protein stabilization; ISO:RGD.
DR GO; GO:0006625; P:protein targeting to peroxisome; ISO:RGD.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00004; AAA; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00674; AAA; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell projection; Cytoplasm; Membrane; Methylation;
KW Nucleotide-binding; Peroxisome; Peroxisome biogenesis; Reference proteome;
KW Repeat.
FT CHAIN 1..978
FT /note="Peroxisome assembly factor 2"
FT /id="PRO_0000084609"
FT BINDING 470..477
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 742..749
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT MOD_RES 119
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0000250|UniProtKB:Q13608"
FT MUTAGEN 476
FT /note="K->A: No loss of function."
FT /evidence="ECO:0000269|PubMed:7493019"
FT MUTAGEN 748
FT /note="K->A: Loss of function."
FT /evidence="ECO:0000269|PubMed:7493019"
FT CONFLICT 299
FT /note="D -> G (in Ref. 2; BAA24931)"
FT /evidence="ECO:0000305"
FT CONFLICT 333
FT /note="V -> A (in Ref. 2; BAA24931)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="Q -> R (in Ref. 2; BAA24931)"
FT /evidence="ECO:0000305"
FT CONFLICT 546
FT /note="R -> C (in Ref. 2; BAA24931)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 978 AA; 104426 MW; F723193B7E95EA97 CRC64;
MALAVLHVLE PFPTETPPLA VLLPPGGPWP VTGVGLVLAL RPASESPAGP ALLVAAVEGS
GAQCEQRGPG PPPLLVSRTL LRVLALSPGA RVRARPVRRP PALGWALLGT SPGPGLGPRV
GPLLVRRGET LPVPGSRVLE TRPALQGLLG PGTRLAVTEL QGRTKLDPES RDHNHPPPPP
VVSSFAVSHS IRQLRGVLGG TGDALGVSRS CLRSLGLFQG EWVWVARVGE LPNTSQPHLA
QVQVLEPRWD LSARLGPNSG QPGEPLADGL VFVPATLAFN LGCDPLEVGE LRIQRYLEDS
TAAEDKGSCS LLPGPPFARE LHIEVLPSPH CGVNGKYDHV LYQHFHTPRV VQEGDVLCVS
TAGQVEILEG SLERLPRWRE VFFKVKKTVG EAPDGPASAF LADTTHTSLY LAGTTLSRVP
PLPSGRSPPW DSLSPPGLEA LVNELCAVLK PHLQPGGTLL TGTSCVLLQG PPGSGKTTAV
TAACSRLGLH LLKVPCSSLC ADSSRTVETK LQTTFSRARR CRPVVLLLTA LDLLGRDRDG
LGEDARVVAT LRHLLLDEDP LSRCPPLMVV ATTSRVQDLP TDVRTAFPHE LEVPVLSESQ
RLSVLQALTA HLPLGQEVNL SQLARRCAGF VVGDLYALLT HASRAACTRI KAAGLAMSEE
DEGELCAAGF PLLAEDFGQA LDQLQTAHSQ AVGAPKIPSV SWHDVGGLQD VKKEILETIQ
LPLEHPELLS LGLRRSGLLL HGPPGTGKTL LAKAVATECS LTFLSVKGPE LINMYVGQSE
ENVREVFARA RAAAPCIIFF DELDSLAPSR GRSGDSGGVM DRVVSQLLAE LDGLHSTQDV
FVIGATNRPD LLDPALLRPG RFDKLVFVGA SEDRASQLRV LSAITRKFKL EASVSLMNVL
DCCPPQLTGA DLYSLCSDAM MTALKRRVRD LEEGLEPRSS ALLLTMEDLL QAAARLQPSV
SEQELLRYKR IQRKFAAC
