ID   PEX8_YEAST              Reviewed;         589 AA.
AC   P53248; D6VUK9;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 140.
DE   RecName: Full=Peroxisomal biogenesis factor 8;
DE   AltName: Full=Peroxin-8;
DE   AltName: Full=Peroxisomal protein PAS6;
GN   Name=PEX8; Synonyms=PAS6; OrderedLocusNames=YGR077C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Required for peroxisome assembly.
CC   -!- SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000305}.
CC   -!- MISCELLANEOUS: Present with 538 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; Z72862; CAA97079.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08170.1; -; Genomic_DNA.
DR   PIR; S64372; S64372.
DR   RefSeq; NP_011591.1; NM_001181206.1.
DR   AlphaFoldDB; P53248; -.
DR   BioGRID; 33319; 174.
DR   DIP; DIP-2478N; -.
DR   IntAct; P53248; 15.
DR   MINT; P53248; -.
DR   STRING; 4932.YGR077C; -.
DR   TCDB; 3.A.20.1.5; the peroxisomal protein importer (ppi) family.
DR   iPTMnet; P53248; -.
DR   PaxDb; P53248; -.
DR   PRIDE; P53248; -.
DR   EnsemblFungi; YGR077C_mRNA; YGR077C; YGR077C.
DR   GeneID; 852968; -.
DR   KEGG; sce:YGR077C; -.
DR   SGD; S000003309; PEX8.
DR   VEuPathDB; FungiDB:YGR077C; -.
DR   eggNOG; ENOG502R1X7; Eukaryota.
DR   HOGENOM; CLU_031057_0_0_1; -.
DR   InParanoid; P53248; -.
DR   OMA; FESHRAT; -.
DR   BioCyc; YEAST:G3O-30789-MON; -.
DR   PRO; PR:P53248; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P53248; protein.
DR   GO; GO:0005780; C:extrinsic component of intraperoxisomal membrane; IDA:SGD.
DR   GO; GO:1990429; C:peroxisomal importomer complex; IDA:SGD.
DR   GO; GO:0005782; C:peroxisomal matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0030674; F:protein-macromolecule adaptor activity; IDA:SGD.
DR   GO; GO:0016558; P:protein import into peroxisome matrix; IMP:SGD.
PE   1: Evidence at protein level;
KW   Peroxisome; Peroxisome biogenesis; Reference proteome.
FT   CHAIN           1..589
FT                   /note="Peroxisomal biogenesis factor 8"
FT                   /id="PRO_0000058343"
FT   MOTIF           587..589
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   589 AA;  68165 MW;  7683417142F81701 CRC64;
     MFDHDVEYLI TALSSETRIQ YDQRLLDEIA ANVVYYVPRV KSPDTLYRLV GALFRSQFIV
     QLPPLRLLHI VKDVFLWKLE VSEPTLPISK FYLVWNAVFE SHRATWNLSQ LMVLDGVLVT
     YPSFKQLNNA YFIDESSNKT ALYYRNWKLQ LFSPIWAQLW NTAIVRANLS IQHCLLIALA
     LLFNQSNRSA LLHGVDVSWN LVTEKLLDLL EEYVHGIVQP MEIFSTDSVL STNLNHLASC
     LTSSITRSNE ATLVNSVRKL ERICRYLSDT VASLKEQQLD FKFQNVFILI ILALKELSAM
     NMTILPNHKD TFYSMICLSL FHVHVLTQKI GTVGFPSYDY VYDNLVTYFI VMDDLSKITT
     VLELMKRNNT KQDPNKLVFY INFLNKITNY YGCRIRLPFI TEFIEPLLHF DVFFSGKTGN
     TLDIEIKESI HTLTITVLSI DSSYSSQVAQ WQVSRILVYL KMSMDQFIAG KLSANQILLI
     FGHLSTQLPS LHNYNKHLLR DSLHETYIRI VNVKNPEKKN VLIECLIVQI AFINNPHHLI
     GWLNICLQLI NTHNKKLLQQ LWEMVSSLES SLAIDWWYTT VLSSQSSKL