ID   PF12_PIG                Reviewed;         228 AA.
AC   P51525;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Prophenin-2;
DE   AltName: Full=C12;
DE   AltName: Full=PF-2;
DE   AltName: Full=PR-2;
DE   AltName: Full=Prophenin-1-like;
DE   Flags: Precursor;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Bone marrow;
RX   PubMed=8262247; DOI=10.1016/0014-5793(93)80821-b;
RA   Pungercar J., Strukelj B., Kopitar G., Renko M., Lenarcic B., Gubensek F.,
RA   Turk V.;
RT   "Molecular cloning of a putative homolog of proline/arginine-rich
RT   antibacterial peptides from porcine bone marrow.";
RL   FEBS Lett. 336:284-288(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Liver;
RX   PubMed=7498526; DOI=10.1016/0014-5793(95)01237-3;
RA   Zhao C., Ganz T., Lehrer R.I.;
RT   "Structures of genes for two cathelin-associated antimicrobial peptides:
RT   prophenin-2 and PR-39.";
RL   FEBS Lett. 376:130-134(1995).
CC   -!- FUNCTION: Exerts antimicrobial activity. It is more effective against
CC       Gram-negative bacteria than Gram-positive bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the cathelicidin family. {ECO:0000305}.
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DR   EMBL; X75438; CAA53188.1; -; mRNA.
DR   EMBL; X89202; CAA61488.1; -; Genomic_DNA.
DR   PIR; S40463; S40463.
DR   RefSeq; NP_999028.1; NM_213863.1.
DR   AlphaFoldDB; P51525; -.
DR   SMR; P51525; -.
DR   STRING; 9823.ENSSSCP00000021393; -.
DR   TCDB; 1.C.33.1.1; the cathelicidin (cathelicidin) family.
DR   PeptideAtlas; P51525; -.
DR   PRIDE; P51525; -.
DR   GeneID; 396871; -.
DR   KEGG; ssc:396871; -.
DR   CTD; 396871; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0001530; F:lipopolysaccharide binding; IBA:GO_Central.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IBA:GO_Central.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IBA:GO_Central.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   InterPro; IPR001894; Cathelicidin-like.
DR   InterPro; IPR018216; Cathelicidin_CS.
DR   InterPro; IPR046350; Cystatin_sf.
DR   PANTHER; PTHR10206; PTHR10206; 1.
DR   SUPFAM; SSF54403; SSF54403; 1.
DR   PROSITE; PS00946; CATHELICIDINS_1; 1.
DR   PROSITE; PS00947; CATHELICIDINS_2; 1.
PE   2: Evidence at transcript level;
KW   Amidation; Antibiotic; Antimicrobial; Disulfide bond; Reference proteome;
KW   Repeat; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   PROPEP          30..146
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000004741"
FT   PEPTIDE         147..225
FT                   /note="Prophenin-2"
FT                   /id="PRO_0000004742"
FT   PROPEP          226..228
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000004743"
FT   REPEAT          148..157
FT                   /note="1"
FT   REPEAT          158..167
FT                   /note="2"
FT   REPEAT          168..177
FT                   /note="3"
FT   REPEAT          178..187
FT                   /note="4"
FT   REPEAT          188..197
FT                   /note="5"
FT   REPEAT          198..207
FT                   /note="6"
FT   REPEAT          208..217
FT                   /note="7"
FT   REGION          148..217
FT                   /note="7 X 10 AA tandem repeats"
FT   REGION          167..195
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          207..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         225
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000255"
FT   DISULFID        85..96
FT                   /evidence="ECO:0000250"
FT   DISULFID        107..124
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   228 AA;  25855 MW;  1EA4511FF35CC182 CRC64;
     METQRASLCL GRWSLWLLLL ALVVPSASAQ ALSYREAVLR AVDRLNEQSS EANLYRLLEL
     DQPPKADEDP GTPKPVSFTV KETVCPRPTR RPPELCDFKE NGRVKQCVGT VTLDQIKDPL
     DITCNEGVRR FPWWWPFLRR PRLRRQAFPP PNVPGPRFPP PNVPGPRFPP PNFPGPRFPP
     PNFPGPRFPP PNFPGPPFPP PIFPGPWFPP PPPFRPPPFG PPRFPGRR