PF36P_PLAF7
ID PF36P_PLAF7 Reviewed; 478 AA.
AC Q8I1Y4;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Sporozoite surface protein P36p;
DE AltName: Full=Sporozoite surface protein P52;
DE Flags: Precursor;
GN Name=PF52; Synonyms=PF36P; ORFNames=PFD0215c;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18958160; DOI=10.1371/journal.pone.0003549;
RA van Schaijk B.C., Janse C.J., van Gemert G.J., van Dijk M.R., Gego A.,
RA Franetich J.F., van de Vegte-Bolmer M., Yalaoui S., Silvie O.,
RA Hoffman S.L., Waters A.P., Mazier D., Sauerwein R.W., Khan S.M.;
RT "Gene disruption of Plasmodium falciparum p52 results in attenuation of
RT malaria liver stage development in cultured primary human hepatocytes.";
RL PLoS ONE 3:E3549-E3549(2008).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19625622; DOI=10.1073/pnas.0906387106;
RA VanBuskirk K.M., O'Neill M.T., De La Vega P., Maier A.G., Krzych U.,
RA Williams J., Dowler M.G., Sacci J.B. Jr., Kangwanrangsan N., Tsuboi T.,
RA Kneteman N.M., Heppner D.G. Jr., Murdock B.A., Mikolajczak S.A., Aly A.S.,
RA Cowman A.F., Kappe S.H.;
RT "Preerythrocytic, live-attenuated Plasmodium falciparum vaccine candidates
RT by design.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:13004-13009(2009).
CC -!- FUNCTION: Involved in sporozoite infection of hepatocytes and
CC replication therein. {ECO:0000269|PubMed:18958160,
CC ECO:0000269|PubMed:19625622}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000250}. Cell membrane
CC {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}. Note=Present on
CC the surface of sporozoites. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Attenuated parasites that cannot commit to
CC infection, even when they encounter with hepatocytes, resulting in
CC continuous traversal of hepatocytes. {ECO:0000269|PubMed:18958160,
CC ECO:0000269|PubMed:19625622}.
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DR EMBL; AL844503; CAD49137.1; -; Genomic_DNA.
DR RefSeq; XP_001351357.1; XM_001351321.1.
DR AlphaFoldDB; Q8I1Y4; -.
DR STRING; 5833.PFD0215c; -.
DR EnsemblProtists; CAD49137; CAD49137; PF3D7_0404500.
DR GeneID; 9221796; -.
DR KEGG; pfa:PF3D7_0404500; -.
DR VEuPathDB; PlasmoDB:PF3D7_0404500; -.
DR HOGENOM; CLU_579377_0_0_1; -.
DR InParanoid; Q8I1Y4; -.
DR OMA; CVIHAYP; -.
DR PhylomeDB; Q8I1Y4; -.
DR Proteomes; UP000001450; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.2860; -; 2.
DR InterPro; IPR010884; 6_CYS_dom.
DR InterPro; IPR038160; 6_CYS_dom_sf.
DR Pfam; PF07422; s48_45; 1.
DR SMART; SM00970; s48_45; 1.
DR PROSITE; PS51701; 6_CYS; 2.
PE 3: Inferred from homology;
KW Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW Malaria; Membrane; Reference proteome; Repeat; Signal.
FT SIGNAL 1..41
FT /evidence="ECO:0000255"
FT CHAIN 42..455
FT /note="Sporozoite surface protein P36p"
FT /id="PRO_0000423573"
FT PROPEP 456..478
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000423574"
FT DOMAIN 42..178
FT /note="6-Cys 1"
FT DOMAIN 181..326
FT /note="6-Cys 2"
FT LIPID 455
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 109
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 138
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 279
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 59..71
FT /evidence="ECO:0000250"
FT DISULFID 85..159
FT /evidence="ECO:0000250"
FT DISULFID 102..157
FT /evidence="ECO:0000250"
FT DISULFID 185..209
FT /evidence="ECO:0000250"
FT DISULFID 223..302
FT /evidence="ECO:0000250"
FT DISULFID 243..300
FT /evidence="ECO:0000250"
SQ SEQUENCE 478 AA; 56287 MW; 8899545622211D90 CRC64;
MYVLVLIHMC YHFTMKRKKL FVYFIFLSFI INFNFNININ FVCSNVIQDV ISIGNVDICV
VNVNSDEAQE CILNNEFGKL LLFVCNMNDA FSTTAKTHPE NCPSRAFVNQ SNPTENSPEV
DTYSIYPNLF GTNENRLNDT YSLYSTPYSN MDIDFSCLCY GDKQDKVKHI MRINIKKTRK
KIKGCDFGDN IPSKRDLTNS LSLNERSSCI IHAYSNDVLG INCFKKEINN SYNNNLELNP
SNCFHDVYFG ADLILNSKNV IPNSRVIPDP SSDVKLSRNH SFSSYLILPN NLTENIKISC
TCKRDEFVGT MIIYTKNINS LMFDNNNNNN DEEQIFQNKY MKKKEYKKDE GNEYDKKMNT
DDNYINNEEH HNNNQYNNYE NKINNVNYNY DDISKYINEH YKNYDHEKNS KNSYKTNTNI
HDQYDTYHYN NKYDLHSDRT RIRTRTFWQN LFGLSSSKYI LFNNFLILFI FLIYIYST
