PF36_PLABA
ID PF36_PLABA Reviewed; 352 AA.
AC Q4Z4D1; Q962H5;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Sporozoite surface protein P36;
DE Flags: Precursor;
GN Name=PBS36; ORFNames=PB000892.00.0;
OS Plasmodium berghei (strain Anka).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX NCBI_TaxID=5823;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ANKA;
RX PubMed=11738705; DOI=10.1016/s0166-6851(01)00377-2;
RA Thompson J., Janse C.J., Waters A.P.;
RT "Comparative genomics in Plasmodium: a tool for the identification of genes
RT and functional analysis.";
RL Mol. Biochem. Parasitol. 118:147-154(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ANKA;
RX PubMed=15637271; DOI=10.1126/science.1103717;
RA Hall N., Karras M., Raine J.D., Carlton J.M., Kooij T.W.A., Berriman M.,
RA Florens L., Janssen C.S., Pain A., Christophides G.K., James K.,
RA Rutherford K., Harris B., Harris D., Churcher C.M., Quail M.A., Ormond D.,
RA Doggett J., Trueman H.E., Mendoza J., Bidwell S.L., Rajandream M.A.,
RA Carucci D.J., Yates J.R. III, Kafatos F.C., Janse C.J., Barrell B.G.,
RA Turner C.M.R., Waters A.P., Sinden R.S.;
RT "A comprehensive survey of the Plasmodium life cycle by genomic,
RT transcriptomic, and proteomic analyses.";
RL Science 307:82-86(2005).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ANKA;
RX PubMed=16313615; DOI=10.1111/j.1365-2958.2005.04801.x;
RA Ishino T., Chinzei Y., Yuda M.;
RT "Two proteins with 6-cys motifs are required for malarial parasites to
RT commit to infection of the hepatocyte.";
RL Mol. Microbiol. 58:1264-1275(2005).
RN [4]
RP DEVELOPMENTAL STAGE.
RC STRAIN=ANKA;
RX PubMed=20386715; DOI=10.1371/journal.ppat.1000853;
RA van Dijk M.R., van Schaijk B.C., Khan S.M., van Dooren M.W., Ramesar J.,
RA Kaczanowski S., van Gemert G.J., Kroeze H., Stunnenberg H.G., Eling W.M.,
RA Sauerwein R.W., Waters A.P., Janse C.J.;
RT "Three members of the 6-cys protein family of Plasmodium play a role in
RT gamete fertility.";
RL PLoS Pathog. 6:E1000853-E1000853(2010).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ANKA;
RX PubMed=23227206; DOI=10.1371/journal.pone.0050772;
RA Ploemen I.H., Croes H.J., van Gemert G.J., Wijers-Rouw M., Hermsen C.C.,
RA Sauerwein R.W.;
RT "Plasmodium berghei Deltap52&p36 parasites develop independent of a
RT parasitophorous vacuole membrane in Huh-7 liver cells.";
RL PLoS ONE 7:E50772-E50772(2012).
CC -!- FUNCTION: Involved in sporozoite infection of hepatocytes and
CC replication therein. {ECO:0000269|PubMed:16313615,
CC ECO:0000269|PubMed:23227206}.
CC -!- SUBCELLULAR LOCATION: Cell membrane. Cell surface. Note=Present on the
CC surface of sporozoite. {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed both in gametocytes and in liver-
CC infective sporozoites. {ECO:0000269|PubMed:20386715}.
CC -!- DISRUPTION PHENOTYPE: Attenuated parasites that cannot commit to
CC infection, even when they encounter with hepatocytes, resulting in
CC continuous traversal of hepatocytes. Cells lacking both PBS36 and
CC P52/PBS36P are severely affected in their capability to develop into
CC liver stage parasites and abort development soon after invasion;
CC possibly due to the absence of a parasitophorous vacuole membrane
CC (PVM). {ECO:0000269|PubMed:16313615, ECO:0000269|PubMed:23227206}.
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DR EMBL; AY033091; AAK57743.1; -; Genomic_DNA.
DR EMBL; CAAI01000664; CAH94852.1; -; Genomic_DNA.
DR AlphaFoldDB; Q4Z4D1; -.
DR SMR; Q4Z4D1; -.
DR STRING; 5821.PBANKA_100210; -.
DR VEuPathDB; PlasmoDB:PBANKA_1002100; -.
DR eggNOG; ENOG502TN9C; Eukaryota.
DR HOGENOM; CLU_786380_0_0_1; -.
DR InParanoid; Q4Z4D1; -.
DR OMA; VGINCIN; -.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.40.2860; -; 2.
DR InterPro; IPR010884; 6_CYS_dom.
DR InterPro; IPR038160; 6_CYS_dom_sf.
DR Pfam; PF07422; s48_45; 1.
DR SMART; SM00970; s48_45; 1.
DR PROSITE; PS51701; 6_CYS; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Malaria; Membrane; Repeat;
KW Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..352
FT /note="Sporozoite surface protein P36"
FT /id="PRO_0000423571"
FT DOMAIN 53..199
FT /note="6-Cys 1"
FT DOMAIN 210..351
FT /note="6-Cys 2"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 111
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 160
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 254
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 80..90
FT /evidence="ECO:0000250"
FT DISULFID 104..179
FT /evidence="ECO:0000250"
FT DISULFID 122..177
FT /evidence="ECO:0000250"
FT DISULFID 214..238
FT /evidence="ECO:0000250"
FT DISULFID 252..333
FT /evidence="ECO:0000250"
FT DISULFID 269..331
FT /evidence="ECO:0000250"
FT CONFLICT 195
FT /note="N -> T (in Ref. 1; AAK57743)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 352 AA; 40684 MW; F2E14E370242B971 CRC64;
MKQYEFARHI NTYFSVAQNM LFSIFLYYAF SLLIFLSIFV FKMRKALYSL LFYMCICLYI
YTPVFMASLK EIEVGNYFIC NLKDYPTGNC SVNHDYNKTI KLLCPIIHNK NNSDKTYDPS
YCFKYDGIKD EFIINNKQNY IHNTLPGVIL KSHIENDTYN LSIYPPFVVK EDITIVCICD
SEKGNEGITP YLKINIKKAH GLNNDLEGDY IKGCDYGNNK GKYQFLTKSI KYTIDPICEI
HAYPGDIVGI NCNNYTTKIQ DVSLEPNSCF GMVYFSILTM VFVKTNVNNV MPNAKYYPDL
ASHHGNQNSK MFLTAYLLIP NEIERDILIY CTCSYNGNKG LAIYHIFSTK ED
