ID   PF36_PLAYO              Reviewed;         310 AA.
AC   Q7RPW4;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Sporozoite surface protein P36;
DE   Flags: Precursor;
GN   Name=P36; ORFNames=PY01341;
OS   Plasmodium yoelii yoelii.
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX   NCBI_TaxID=73239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=17XNL;
RX   PubMed=12368865; DOI=10.1038/nature01099;
RA   Carlton J.M., Angiuoli S.V., Suh B.B., Kooij T.W., Pertea M., Silva J.C.,
RA   Ermolaeva M.D., Allen J.E., Selengut J.D., Koo H.L., Peterson J.D., Pop M.,
RA   Kosack D.S., Shumway M.F., Bidwell S.L., Shallom S.J., van Aken S.E.,
RA   Riedmuller S.B., Feldblyum T.V., Cho J.K., Quackenbush J., Sedegah M.,
RA   Shoaibi A., Cummings L.M., Florens L., Yates J.R. III, Raine J.D.,
RA   Sinden R.E., Harris M.A., Cunningham D.A., Preiser P.R., Bergman L.W.,
RA   Vaidya A.B., van Lin L.H., Janse C.J., Waters A.P., Smith H.O., White O.R.,
RA   Salzberg S.L., Venter J.C., Fraser C.M., Hoffman S.L., Gardner M.J.,
RA   Carucci D.J.;
RT   "Genome sequence and comparative analysis of the model rodent malaria
RT   parasite Plasmodium yoelii yoelii.";
RL   Nature 419:512-519(2002).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=17517871; DOI=10.1128/iai.00225-07;
RA   Labaied M., Harupa A., Dumpit R.F., Coppens I., Mikolajczak S.A.,
RA   Kappe S.H.;
RT   "Plasmodium yoelii sporozoites with simultaneous deletion of P52 and P36
RT   are completely attenuated and confer sterile immunity against infection.";
RL   Infect. Immun. 75:3758-3768(2007).
CC   -!- FUNCTION: Involved in sporozoite infection of hepatocytes and
CC       replication therein. {ECO:0000305|PubMed:17517871}.
CC   -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000250}. Cell membrane
CC       {ECO:0000250}. Note=Present on the surface of sporozoite.
CC       {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking both P36 and P52/P36P show
CC       attenuated infection and do not form a parasitophorous vacuole within
CC       hepatocytes. {ECO:0000269|PubMed:17517871}.
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DR   EMBL; AABL01000353; EAA20644.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q7RPW4; -.
DR   SMR; Q7RPW4; -.
DR   DIP; DIP-61878N; -.
DR   STRING; 73239.Q7RPW4; -.
DR   EnsemblProtists; EAA20644; EAA20644; EAA20644.
DR   InParanoid; Q7RPW4; -.
DR   OMA; VGINCIN; -.
DR   Proteomes; UP000008553; Unassembled WGS sequence.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.2860; -; 2.
DR   InterPro; IPR010884; 6_CYS_dom.
DR   InterPro; IPR038160; 6_CYS_dom_sf.
DR   Pfam; PF07422; s48_45; 1.
DR   SMART; SM00970; s48_45; 1.
DR   PROSITE; PS51701; 6_CYS; 2.
PE   3: Inferred from homology;
KW   Cell membrane; Disulfide bond; Glycoprotein; Malaria; Membrane;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..310
FT                   /note="Sporozoite surface protein P36"
FT                   /id="PRO_0000423572"
FT   DOMAIN          25..157
FT                   /note="6-Cys 1"
FT   DOMAIN          168..309
FT                   /note="6-Cys 2"
FT   CARBOHYD        72
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        114
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        118
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        290
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        38..48
FT                   /evidence="ECO:0000250"
FT   DISULFID        62..137
FT                   /evidence="ECO:0000250"
FT   DISULFID        80..135
FT                   /evidence="ECO:0000250"
FT   DISULFID        172..196
FT                   /evidence="ECO:0000250"
FT   DISULFID        210..291
FT                   /evidence="ECO:0000250"
FT   DISULFID        227..289
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   310 AA;  35356 MW;  18E3AC7D54007627 CRC64;
     MRKALYSLLF YMCICLYIYT PVFMANLKEI EVGNYFICNL RDYPTGNCSV DHDYNKTIKL
     LCPIVNNKNN SNKTYDPSYC FKYDGIKDEF IINNKQAYIH NTLPGVILTN NIENDTYNLS
     IYPPFVVKED VTIVCICDSE KGNEGITPYL KINIKKTHGL NNDLEGDYIK GCDYGNNQGK
     YKFLTKPVKY TSNPICEIDA YPGDVVGINC NSYTTKMQGA RLEPEGCFAM VYFSILTMKF
     IKTNVNNIMP NAKYYPDLAS HPGNQNSKIF LTSYLLIPNE VDRDILIYCN CSYNGNKGLA
     IYRIFSTKAS