PF41_PLAF7
ID PF41_PLAF7 Reviewed; 378 AA.
AC Q8I1Y0;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Merozoite surface protein P41;
DE Contains:
DE RecName: Full=Merozoite surface protein P41, processed form;
DE Flags: Precursor;
GN Name=PF41; ORFNames=PFD0240c;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=16203726; DOI=10.1074/jbc.m509631200;
RA Sanders P.R., Gilson P.R., Cantin G.T., Greenbaum D.C., Nebl T.,
RA Carucci D.J., McConville M.J., Schofield L., Hodder A.N., Yates J.R. III,
RA Crabb B.S.;
RT "Distinct protein classes including novel merozoite surface antigens in
RT Raft-like membranes of Plasmodium falciparum.";
RL J. Biol. Chem. 280:40169-40176(2005).
RN [4]
RP SUBCELLULAR LOCATION, CLEAVAGE, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND INTERACTION WITH PF12.
RX PubMed=22848665; DOI=10.1371/journal.pone.0041937;
RA Taechalertpaisarn T., Crosnier C., Bartholdson S.J., Hodder A.N.,
RA Thompson J., Bustamante L.Y., Wilson D.W., Sanders P.R., Wright G.J.,
RA Rayner J.C., Cowman A.F., Gilson P.R., Crabb B.S.;
RT "Biochemical and functional analysis of two Plasmodium falciparum blood-
RT stage 6-cys proteins: P12 and P41.";
RL PLoS ONE 7:E41937-E41937(2012).
RN [5]
RP INTERACTION WITH PF12.
RX PubMed=23511632; DOI=10.1074/jbc.m113.455667;
RA Tonkin M.L., Arredondo S.A., Loveless B.C., Serpa J.J., Makepeace K.A.,
RA Sundar N., Petrotchenko E.V., Miller L.H., Grigg M.E., Boulanger M.J.;
RT "Structural and biochemical characterization of Plasmodium falciparum 12
RT (Pf12) reveals a unique interdomain organization and the potential for an
RT antiparallel arrangement with Pf41.";
RL J. Biol. Chem. 288:12805-12817(2013).
CC -!- SUBUNIT: Heterodimer; heterodimerizes with PF12. May form an
CC antiparallel heterodimer with PF12.
CC -!- SUBCELLULAR LOCATION: Cell surface. Cell membrane. Note=Present on the
CC surface of merozoite. {ECO:0000269|PubMed:22848665}.
CC -!- DEVELOPMENTAL STAGE: Specifically present in asexual blood stage
CC parasites. First detected in the trophozoite stage, 30-40 hours post-
CC invasion (HPI) and the proteins reaches peak expression in schizont
CC stage, 40-48 HPI. {ECO:0000269|PubMed:16203726,
CC ECO:0000269|PubMed:22848665}.
CC -!- PTM: Processed into a soluble form. {ECO:0000269|PubMed:22848665}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Parasites grow at normal
CC rates in vitro. {ECO:0000269|PubMed:22848665}.
CC -!- MISCELLANEOUS: Does not have erythrocyte-receptor-binding ability.
CC {ECO:0000305|PubMed:22848665}.
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DR EMBL; AL844503; CAD49141.1; -; Genomic_DNA.
DR RefSeq; XP_001351361.1; XM_001351325.1.
DR PDB; 4YS4; X-ray; 2.45 A; A=21-378.
DR PDB; 7S7Q; X-ray; 2.85 A; A=20-368.
DR PDBsum; 4YS4; -.
DR PDBsum; 7S7Q; -.
DR AlphaFoldDB; Q8I1Y0; -.
DR SMR; Q8I1Y0; -.
DR BioGRID; 1207785; 1.
DR STRING; 5833.PFD0240c; -.
DR PRIDE; Q8I1Y0; -.
DR EnsemblProtists; CAD49141; CAD49141; PF3D7_0404900.
DR GeneID; 812447; -.
DR KEGG; pfa:PF3D7_0404900; -.
DR VEuPathDB; PlasmoDB:PF3D7_0404900; -.
DR HOGENOM; CLU_693510_0_0_1; -.
DR InParanoid; Q8I1Y0; -.
DR OMA; CRIEAKP; -.
DR PhylomeDB; Q8I1Y0; -.
DR Proteomes; UP000001450; Chromosome 4.
DR GO; GO:0046658; C:anchored component of plasma membrane; IDA:GeneDB.
DR GO; GO:0009986; C:cell surface; IDA:GeneDB.
DR GO; GO:0020004; C:symbiont-containing vacuolar space; IDA:GeneDB.
DR Gene3D; 2.60.40.2860; -; 2.
DR InterPro; IPR010884; 6_CYS_dom.
DR InterPro; IPR038160; 6_CYS_dom_sf.
DR Pfam; PF07422; s48_45; 2.
DR SMART; SM00970; s48_45; 2.
DR PROSITE; PS51701; 6_CYS; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Disulfide bond; Glycoprotein; Malaria;
KW Membrane; Reference proteome; Repeat; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..378
FT /note="Merozoite surface protein P41"
FT /id="PRO_0000423583"
FT CHAIN 21..?
FT /note="Merozoite surface protein P41, processed form"
FT /id="PRO_0000423584"
FT DOMAIN 21..133
FT /note="6-Cys 1"
FT DOMAIN 241..375
FT /note="6-Cys 2"
FT CARBOHYD 77
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 149
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 205
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 351
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 25..42
FT /evidence="ECO:0000250"
FT DISULFID 56..113
FT /evidence="ECO:0000250"
FT DISULFID 64..111
FT /evidence="ECO:0000250"
FT DISULFID 245..270
FT /evidence="ECO:0000250"
FT DISULFID 284..348
FT /evidence="ECO:0000250"
FT DISULFID 297..346
FT /evidence="ECO:0000250"
FT STRAND 22..26
FT /evidence="ECO:0007829|PDB:4YS4"
FT HELIX 30..33
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 36..46
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 51..55
FT /evidence="ECO:0007829|PDB:4YS4"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 80..82
FT /evidence="ECO:0007829|PDB:4YS4"
FT HELIX 83..86
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 96..98
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 107..115
FT /evidence="ECO:0007829|PDB:4YS4"
FT HELIX 117..126
FT /evidence="ECO:0007829|PDB:4YS4"
FT HELIX 127..129
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 227..235
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 242..246
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 248..250
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 255..261
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 269..274
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 279..283
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 291..294
FT /evidence="ECO:0007829|PDB:4YS4"
FT TURN 295..299
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 300..303
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 306..309
FT /evidence="ECO:0007829|PDB:4YS4"
FT HELIX 310..312
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 318..320
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 326..328
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 330..333
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 336..338
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 342..349
FT /evidence="ECO:0007829|PDB:4YS4"
FT HELIX 353..355
FT /evidence="ECO:0007829|PDB:4YS4"
FT STRAND 358..363
FT /evidence="ECO:0007829|PDB:4YS4"
SQ SEQUENCE 378 AA; 43088 MW; 2F8FEBCF82F81863 CRC64;
MKGVIFCLVV LLWRQAWVSS KSHKCDFTKE KYLLSGEKEV SCEIDANPSD DITFICPNKI
DSLCFHTVNI SKNINQNKST MSIQDLLYGS VVYGNTLFIS PYVRTNTPFY CFCNLDTVTI
QKFLKINRFL KDDDELSEAD VMKHLKGGNV SEAQADEYLN KALNRFKKMK DLSKFFNDQA
DNTTKLNLPK SLNIPNDILN YDVYNSSNNR NDIVVKDEVT NKQIISKRGI MSVFVRSNNN
VIKGCDFGNN NKNYFSHPIS VAGKVNNKVC KIQGKPGELV GFKCAFEENG KVEPPNCFDQ
VLHKNKVTDL KTLIPGYASY TNKHSSKYPY YLKIPHFVNE QYTIQCKCKS NNSQNEYTFE
LDIQPGESEV VLNSFKTS
