ID   PFA5_ASHGO              Reviewed;         359 AA.
AC   Q75D19;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Palmitoyltransferase PFA5;
DE            EC=2.3.1.225;
DE   AltName: Full=Protein fatty acyltransferase 5;
GN   Name=PFA5; OrderedLocusNames=ABR203W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 241.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC         hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC         COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC         EC=2.3.1.225;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC       {ECO:0000250}.
CC   -!- PTM: Autopalmitoylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family. PFA5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE016815; AAS50976.2; -; Genomic_DNA.
DR   RefSeq; NP_983152.2; NM_208505.2.
DR   AlphaFoldDB; Q75D19; -.
DR   STRING; 33169.AAS50976; -.
DR   EnsemblFungi; AAS50976; AAS50976; AGOS_ABR203W.
DR   GeneID; 4619262; -.
DR   KEGG; ago:AGOS_ABR203W; -.
DR   eggNOG; KOG1311; Eukaryota.
DR   HOGENOM; CLU_064801_0_0_1; -.
DR   InParanoid; Q75D19; -.
DR   OMA; YCVWIGT; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR   GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR   GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR   InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR   Pfam; PF01529; DHHC; 1.
DR   PROSITE; PS50216; DHHC; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..359
FT                   /note="Palmitoyltransferase PFA5"
FT                   /id="PRO_0000212973"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        206..226
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          120..170
FT                   /note="DHHC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
SQ   SEQUENCE   359 AA;  41610 MW;  959886B9706059CF CRC64;
     MWQCKNKSRW WYSLVPILCI CIMCYGAIAY SYSFCYVEVW THLGMKAAAI GMTCLHLIIV
     ILLWIIWAQI IMMGPGRQPR VAPFMILPEM ADAGERAKEG AATSVLPPDV YQCDTQGYPV
     WCSVCQSLKG LRTHHSVHLG FCVPRLDHYC VWLGTVIGRR NYRLFNQFLM CFLMHALIIF
     VSVVSLQRRI ASSARMRGER EDPNVLVVLS LSCLVLLMVG ALFISFLNYM ANNQTTIEKL
     YTPKRQPRTM CFCVYNPADQ YRYVVKSLPH ENWNMWDKGS AYANYKDFLG SSIWRWFIPI
     GSNIPEFQTS AWEDDYNAIL GPYKEELGSH YRDILMQRIE QGKYVTRLRV YGDKFREGL