ID   PFA5_CANAL              Reviewed;         405 AA.
AC   Q59NR8; A0A1D8PN74;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 2.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Palmitoyltransferase PFA5;
DE            EC=2.3.1.225;
DE   AltName: Full=Protein fatty acyltransferase 5;
GN   Name=PFA5; OrderedLocusNames=CAALFM_C501440CA;
GN   ORFNames=CaO19.11611, CaO19.4134;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC         hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC         COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC         EC=2.3.1.225;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC       {ECO:0000250}.
CC   -!- PTM: Autopalmitoylated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family. PFA5
CC       subfamily. {ECO:0000305}.
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DR   EMBL; CP017627; AOW29577.1; -; Genomic_DNA.
DR   RefSeq; XP_711338.2; XM_706246.2.
DR   AlphaFoldDB; Q59NR8; -.
DR   STRING; 237561.Q59NR8; -.
DR   PRIDE; Q59NR8; -.
DR   GeneID; 3647043; -.
DR   KEGG; cal:CAALFM_C501440CA; -.
DR   CGD; CAL0000196002; orf19.11611.
DR   VEuPathDB; FungiDB:C5_01440C_A; -.
DR   eggNOG; KOG1311; Eukaryota.
DR   HOGENOM; CLU_715709_0_0_1; -.
DR   InParanoid; Q59NR8; -.
DR   OrthoDB; 1491968at2759; -.
DR   PRO; PR:Q59NR8; -.
DR   Proteomes; UP000000559; Chromosome 5.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IBA:GO_Central.
DR   GO; GO:0018230; P:peptidyl-L-cysteine S-palmitoylation; IBA:GO_Central.
DR   GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR   InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR   Pfam; PF01529; DHHC; 1.
DR   PROSITE; PS50216; DHHC; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Lipoprotein; Membrane; Palmitate; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..405
FT                   /note="Palmitoyltransferase PFA5"
FT                   /id="PRO_0000212975"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        310..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          111..161
FT                   /note="DHHC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00067"
SQ   SEQUENCE   405 AA;  47820 MW;  1F82ED0B6C6F762E CRC64;
     MIWRKEYLKK NYIKLLVPIC VVLVLAYLNY AINYAVGYKL VYVHHSHAVA IILWVLLGFF
     QLELLVYWVL IFLVGPGKSP VFPPIDLYSE NNKGLIPLPD LFFCDEKGFP YYCSNSNSIK
     LERSFFSKDV GYNVIKFDHY CIWIGQPIGQ DNYLFFMKFM MGFLAFFIIV LIYCARFTRE
     SIQQGEIDHN FIVLFVMSGF WIIMIGCLFG IHLRYVSINM TTLDEITINQ RKRYNRWKDA
     RKNPNMPSWM KTKNPPRKET GRKYVNVKHK TGRAIVRYYI DERPFDMGFR RNWINLVFNG
     NRNHGKDDEF YTLWRLAAAF VVFIIPFIDI PFSFRGKLQV KDDVEQELHE QENLLAKYTV
     YSSVVNDKFM NMIDEKLKKN SYSAPGYLVE TTPQNNQSTI DKDSI