ID   PFAP_LEPBO              Reviewed;         204 AA.
AC   Q48513;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Putative peptidase PfaP;
DE            EC=3.4.21.-;
DE   AltName: Full=PF-associated peptidase;
DE   AltName: Full=Periplasmic flagella-associated protein;
DE   Flags: Precursor;
GN   Name=pfaP;
OS   Leptospira borgpetersenii.
OC   Bacteria; Spirochaetes; Leptospirales; Leptospiraceae; Leptospira.
OX   NCBI_TaxID=174;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RZ33 / Serovar Hardjobovis;
RX   PubMed=7628708; DOI=10.1016/0378-1119(95)00201-g;
RA   Trueba G.A., Bolin C.A., Zuerner R.L.;
RT   "Cloning of the pfaP gene of Leptospira borgpetersenii.";
RL   Gene 160:133-134(1995).
CC   -!- FUNCTION: Possible protease. May be involved in export of periplasmic
CC       flagella proteins.
CC   -!- SIMILARITY: Belongs to the peptidase S49 family. {ECO:0000305}.
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DR   EMBL; L27482; AAA79884.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48513; -.
DR   SMR; Q48513; -.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd07023; S49_Sppa_N_C; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR004635; Pept_S49_SppA.
DR   InterPro; IPR002142; Peptidase_S49.
DR   Pfam; PF01343; Peptidase_S49; 1.
DR   SUPFAM; SSF52096; SSF52096; 1.
DR   TIGRFAMs; TIGR00706; SppA_dom; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Protease; Serine protease; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..204
FT                   /note="Putative peptidase PfaP"
FT                   /id="PRO_0000027357"
FT   ACT_SITE        19
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        70
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   204 AA;  23354 MW;  FA24A3A75714770B CRC64;
     MRLRKTRKIV VSMKDMAASG GYYIASSADK IFALSGTITG SIGVLQWLRY QRAFGSLGVK
     MRTYKEGKYK DSLSLFRDST PEEDEMIQKM LSDTYNEFVQ DVAKGRNQTV KSVQNLAEGR
     IYSGQDAFRN KLVDEIGGRK EALEELSRLC QYDGEIPLYE EEESPFDRLF MMLGSKMNSF
     SSERIFFREF KNSPVLVILP QAIR