PFD1_YEAST
ID PFD1_YEAST Reviewed; 109 AA.
AC P46988; D6VW10; E9P8V7;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Prefoldin subunit 1;
DE AltName: Full=Genes involved in microtubule biogenesis protein 6;
DE AltName: Full=Gim complex subunit 6;
DE Short=GimC subunit 6;
GN Name=PFD1; Synonyms=GIM6; OrderedLocusNames=YJL179W; ORFNames=J0488;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 74.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION IN THE PREFOLDIN COMPLEX.
RX PubMed=9878052; DOI=10.1093/emboj/18.1.75;
RA Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E.,
RA Hartl F.U.;
RT "Compartmentation of protein folding in vivo: sequestration of non-native
RT polypeptide by the chaperonin-GimC system.";
RL EMBO J. 18:75-84(1999).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Binds specifically to cytosolic chaperonin (c-CPN) and
CC transfers target proteins to it. Binds to nascent polypeptide chain and
CC promotes folding in an environment in which there are many competing
CC pathways for nonnative proteins.
CC -!- SUBUNIT: Heterohexamer of two PFD-alpha type and four PFD-beta type
CC subunits. {ECO:0000269|PubMed:9878052}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 721 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the prefoldin subunit beta family.
CC {ECO:0000305}.
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DR EMBL; Z49454; CAA89474.1; -; Genomic_DNA.
DR EMBL; AY558253; AAS56579.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08626.2; -; Genomic_DNA.
DR PIR; S56962; S56962.
DR RefSeq; NP_012356.2; NM_001181612.2.
DR AlphaFoldDB; P46988; -.
DR SMR; P46988; -.
DR BioGRID; 33582; 291.
DR ComplexPortal; CPX-1671; Prefoldin co-chaperone complex.
DR DIP; DIP-2110N; -.
DR IntAct; P46988; 3.
DR MINT; P46988; -.
DR STRING; 4932.YJL179W; -.
DR iPTMnet; P46988; -.
DR MaxQB; P46988; -.
DR PaxDb; P46988; -.
DR PRIDE; P46988; -.
DR EnsemblFungi; YJL179W_mRNA; YJL179W; YJL179W.
DR GeneID; 853260; -.
DR KEGG; sce:YJL179W; -.
DR SGD; S000003715; PFD1.
DR VEuPathDB; FungiDB:YJL179W; -.
DR eggNOG; ENOG502S3UF; Eukaryota.
DR HOGENOM; CLU_122140_1_1_1; -.
DR InParanoid; P46988; -.
DR OMA; WRSCGKM; -.
DR BioCyc; YEAST:G3O-31614-MON; -.
DR PRO; PR:P46988; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P46988; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016272; C:prefoldin complex; IMP:SGD.
DR GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; IMP:SGD.
DR GO; GO:0007010; P:cytoskeleton organization; IMP:SGD.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
DR GO; GO:0006457; P:protein folding; IMP:SGD.
DR Gene3D; 1.10.287.370; -; 1.
DR InterPro; IPR002777; PFD_beta-like.
DR InterPro; IPR009053; Prefoldin.
DR Pfam; PF01920; Prefoldin_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chaperone; Cytoplasm; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..109
FT /note="Prefoldin subunit 1"
FT /id="PRO_0000124834"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CONFLICT 74
FT /note="D -> A (in Ref. 1; CAA89474)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 109 AA; 12782 MW; C4CEF93E67D6CADC CRC64;
MSQIAQEMTV SLRNARTQLD MVNQQLAYLD RQEKLAELTK KELESYPTDK VWRSCGKSFI
LQDKSKYVND LSHDETVLLD QRKTLKIKKN YLETTVEKTI DNLKALMKN
