PFD2_YEAST
ID PFD2_YEAST Reviewed; 111 AA.
AC P40005; A2TBP1; D3DLP4;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Prefoldin subunit 2;
DE AltName: Full=Genes involved in microtubule biogenesis protein 4;
DE AltName: Full=Gim complex subunit 4;
DE Short=GimC subunit 4;
GN Name=GIM4; Synonyms=PFD2; OrderedLocusNames=YEL003W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-59.
RC STRAIN=ATCC 201390 / BY4743;
RX PubMed=17244705; DOI=10.1073/pnas.0610354104;
RA Juneau K., Palm C., Miranda M., Davis R.W.;
RT "High-density yeast-tiling array reveals previously undiscovered introns
RT and extensive regulation of meiotic splicing.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 7-111.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=9463374; DOI=10.1093/emboj/17.4.952;
RA Geissler S., Siegers K., Schiebel E.;
RT "A novel protein complex promoting formation of functional alpha- and
RT gamma-tubulin.";
RL EMBO J. 17:952-966(1998).
RN [6]
RP IDENTIFICATION IN THE PREFOLDIN COMPLEX.
RX PubMed=9878052; DOI=10.1093/emboj/18.1.75;
RA Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E.,
RA Hartl F.U.;
RT "Compartmentation of protein folding in vivo: sequestration of non-native
RT polypeptide by the chaperonin-GimC system.";
RL EMBO J. 18:75-84(1999).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP IDENTIFICATION OF INTRON.
RX PubMed=17101987; DOI=10.1073/pnas.0605645103;
RA Miura F., Kawaguchi N., Sese J., Toyoda A., Hattori M., Morishita S.,
RA Ito T.;
RT "A large-scale full-length cDNA analysis to explore the budding yeast
RT transcriptome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:17846-17851(2006).
CC -!- FUNCTION: Binds specifically to cytosolic chaperonin (c-CPN) and
CC transfers target proteins to it. Binds to nascent polypeptide chain and
CC promotes folding in an environment in which there are many competing
CC pathways for nonnative proteins.
CC -!- SUBUNIT: Heterohexamer of two PFD-alpha type and four PFD-beta type
CC subunits. {ECO:0000269|PubMed:9878052}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 1660 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the prefoldin subunit beta family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB64480.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAS56665.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U18530; AAB64480.1; ALT_SEQ; Genomic_DNA.
DR EMBL; EF123144; ABM97488.1; -; mRNA.
DR EMBL; AY558339; AAS56665.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006939; DAA07648.1; -; Genomic_DNA.
DR PIR; S50456; S50456.
DR RefSeq; NP_010913.2; NM_001178818.1.
DR AlphaFoldDB; P40005; -.
DR SMR; P40005; -.
DR BioGRID; 36728; 588.
DR ComplexPortal; CPX-1671; Prefoldin co-chaperone complex.
DR DIP; DIP-7472N; -.
DR IntAct; P40005; 1.
DR MINT; P40005; -.
DR STRING; 4932.YEL003W; -.
DR iPTMnet; P40005; -.
DR MaxQB; P40005; -.
DR PaxDb; P40005; -.
DR PRIDE; P40005; -.
DR EnsemblFungi; YEL003W_mRNA; YEL003W; YEL003W.
DR GeneID; 856715; -.
DR KEGG; sce:YEL003W; -.
DR SGD; S000000729; GIM4.
DR VEuPathDB; FungiDB:YEL003W; -.
DR eggNOG; KOG4098; Eukaryota.
DR GeneTree; ENSGT00390000009272; -.
DR HOGENOM; CLU_113004_1_1_1; -.
DR InParanoid; P40005; -.
DR OMA; RKCFRQI; -.
DR BioCyc; YEAST:G3O-30132-MON; -.
DR PRO; PR:P40005; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40005; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0016272; C:prefoldin complex; IPI:SGD.
DR GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR GO; GO:0015631; F:tubulin binding; IDA:SGD.
DR GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR GO; GO:0007021; P:tubulin complex assembly; IMP:SGD.
DR Gene3D; 1.10.287.370; -; 1.
DR InterPro; IPR027235; PFD2.
DR InterPro; IPR002777; PFD_beta-like.
DR InterPro; IPR009053; Prefoldin.
DR PANTHER; PTHR13303; PTHR13303; 1.
DR Pfam; PF01920; Prefoldin_2; 1.
PE 1: Evidence at protein level;
KW Chaperone; Coiled coil; Cytoplasm; Reference proteome.
FT CHAIN 1..111
FT /note="Prefoldin subunit 2"
FT /id="PRO_0000124841"
FT COILED 1..36
FT /evidence="ECO:0000255"
FT COILED 72..92
FT /evidence="ECO:0000255"
FT CONFLICT 59
FT /note="A -> T (in Ref. 3; ABM97488)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 111 AA; 12994 MW; E12DC1BE53C8C936 CRC64;
MEQRNNVFQA KYNEYKQILE ELQTKIIELG HDKDEHTIVI KTLKDAEPTR KCYRMIGGAL
VESDVQTSLP ILETKKENIE GTISKMKETL IQTAKEFEKW KKDNKIQVVK N
