PFD3_MOUSE
ID PFD3_MOUSE Reviewed; 196 AA.
AC P61759; O55228; Q15765; Q3U6M9; Q9CPZ0;
DT 07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Prefoldin subunit 3;
DE AltName: Full=von Hippel-Lindau-binding protein 1;
DE Short=VBP-1;
DE Short=VHL-binding protein 1;
GN Name=Vbp1; Synonyms=Pfdn3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Kidney, Stomach, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 6-196.
RC STRAIN=BALB/cJ; TISSUE=Brain;
RX PubMed=9339366; DOI=10.1006/geno.1997.4902;
RA Brinke A., Green P.M., Giannelli F.;
RT "Characterization of the gene (VBP1) and transcript for the von Hippel-
RT Lindau binding protein and isolation of the highly conserved murine
RT homologue.";
RL Genomics 45:105-112(1997).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Binds specifically to cytosolic chaperonin (c-CPN) and
CC transfers target proteins to it. Binds to nascent polypeptide chain and
CC promotes folding in an environment in which there are many competing
CC pathways for nonnative proteins (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterohexamer of two PFD-alpha type and four PFD-beta type
CC subunits. Binds to the C-terminal part of VHL.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=In complex with VHL can
CC translocate to the nucleus.
CC -!- SIMILARITY: Belongs to the prefoldin subunit alpha family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC23908.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK002282; BAB21986.1; -; mRNA.
DR EMBL; AK008847; BAB25926.1; -; mRNA.
DR EMBL; AK077923; BAC37066.1; -; mRNA.
DR EMBL; AK151527; BAE30475.1; -; mRNA.
DR EMBL; AK153069; BAE31695.1; -; mRNA.
DR EMBL; AK159284; BAE34961.1; -; mRNA.
DR EMBL; AK167064; BAE39225.1; -; mRNA.
DR EMBL; AK169185; BAE40962.1; -; mRNA.
DR EMBL; U96760; AAC23908.1; ALT_INIT; mRNA.
DR CCDS; CCDS41032.1; -.
DR RefSeq; NP_035822.2; NM_011692.2.
DR AlphaFoldDB; P61759; -.
DR SMR; P61759; -.
DR BioGRID; 204503; 27.
DR IntAct; P61759; 10.
DR STRING; 10090.ENSMUSP00000033540; -.
DR iPTMnet; P61759; -.
DR PhosphoSitePlus; P61759; -.
DR REPRODUCTION-2DPAGE; IPI00121851; -.
DR REPRODUCTION-2DPAGE; P61759; -.
DR EPD; P61759; -.
DR MaxQB; P61759; -.
DR PaxDb; P61759; -.
DR PRIDE; P61759; -.
DR ProteomicsDB; 287918; -.
DR Antibodypedia; 3341; 385 antibodies from 29 providers.
DR DNASU; 22327; -.
DR Ensembl; ENSMUST00000033540; ENSMUSP00000033540; ENSMUSG00000031197.
DR GeneID; 22327; -.
DR KEGG; mmu:22327; -.
DR UCSC; uc009tqd.1; mouse.
DR CTD; 7411; -.
DR MGI; MGI:1333804; Vbp1.
DR VEuPathDB; HostDB:ENSMUSG00000031197; -.
DR eggNOG; KOG3313; Eukaryota.
DR GeneTree; ENSGT00390000018904; -.
DR HOGENOM; CLU_083737_1_0_1; -.
DR InParanoid; P61759; -.
DR OMA; DEQHSKY; -.
DR OrthoDB; 1567796at2759; -.
DR PhylomeDB; P61759; -.
DR TreeFam; TF313706; -.
DR BioGRID-ORCS; 22327; 12 hits in 73 CRISPR screens.
DR ChiTaRS; Vbp1; mouse.
DR PRO; PR:P61759; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; P61759; protein.
DR Bgee; ENSMUSG00000031197; Expressed in superior cervical ganglion and 269 other tissues.
DR ExpressionAtlas; P61759; baseline and differential.
DR Genevisible; P61759; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005634; C:nucleus; TAS:MGI.
DR GO; GO:0005844; C:polysome; IBA:GO_Central.
DR GO; GO:0016272; C:prefoldin complex; ISO:MGI.
DR GO; GO:0001540; F:amyloid-beta binding; ISO:MGI.
DR GO; GO:0015631; F:tubulin binding; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; ISO:MGI.
DR GO; GO:0007017; P:microtubule-based process; IBA:GO_Central.
DR GO; GO:1905907; P:negative regulation of amyloid fibril formation; ISO:MGI.
DR GO; GO:0006457; P:protein folding; ISO:MGI.
DR GO; GO:0007021; P:tubulin complex assembly; IBA:GO_Central.
DR Gene3D; 1.10.287.370; -; 1.
DR InterPro; IPR016655; PFD3.
DR InterPro; IPR009053; Prefoldin.
DR InterPro; IPR004127; Prefoldin_subunit_alpha.
DR PANTHER; PTHR12409; PTHR12409; 1.
DR Pfam; PF02996; Prefoldin; 1.
DR PIRSF; PIRSF016396; Prefoldin_subunit_3; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chaperone; Cytoplasm; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P61758"
FT CHAIN 2..196
FT /note="Prefoldin subunit 3"
FT /id="PRO_0000153653"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P61758"
FT MOD_RES 58
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P61758"
SQ SEQUENCE 196 AA; 22436 MW; E5B9179296418DDE CRC64;
MAAAKDGCGL ETAAGNGRRL HLGIPEAVFV EDVDSFMKQP GNETADTVLK KLDEQYQKYK
FMELNLAQKK RRLKGQIPEI KQTLEILKYM QKKKESTNSM ETRFLLADNL YCKASVPPTD
KVCLWLGANV MLEYDIDEAQ ALLEKNLSTA TKNLDSLEED LDFLRDQFTT TEVNMARVYN
WDVKRRNKDD STKNKA
