PFD3_YEAST
ID PFD3_YEAST Reviewed; 199 AA.
AC P48363; D6VUL0;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Prefoldin subunit 3;
DE AltName: Full=Genes involved in microtubule biogenesis protein 2;
DE AltName: Full=Gim complex subunit 2;
DE Short=GimC subunit 2;
GN Name=PAC10; Synonyms=GIM2, PFD3; OrderedLocusNames=YGR078C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Geiser J.R., Hoyt M.A.;
RL Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=9463374; DOI=10.1093/emboj/17.4.952;
RA Geissler S., Siegers K., Schiebel E.;
RT "A novel protein complex promoting formation of functional alpha- and
RT gamma-tubulin.";
RL EMBO J. 17:952-966(1998).
RN [6]
RP IDENTIFICATION IN THE PREFOLDIN COMPLEX.
RX PubMed=9878052; DOI=10.1093/emboj/18.1.75;
RA Siegers K., Waldmann T., Leroux M.R., Grein K., Shevchenko A., Schiebel E.,
RA Hartl F.U.;
RT "Compartmentation of protein folding in vivo: sequestration of non-native
RT polypeptide by the chaperonin-GimC system.";
RL EMBO J. 18:75-84(1999).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Binds specifically to cytosolic chaperonin (c-CPN) and
CC transfers target proteins to it. Binds to nascent polypeptide chain and
CC promotes folding in an environment in which there are many competing
CC pathways for nonnative proteins.
CC -!- SUBUNIT: Heterohexamer of two PFD-alpha type and four PFD-beta type
CC subunits. {ECO:0000269|PubMed:9878052}.
CC -!- INTERACTION:
CC P48363; P53900: GIM3; NbExp=3; IntAct=EBI-13239, EBI-13246;
CC P48363; Q04493: GIM5; NbExp=5; IntAct=EBI-13239, EBI-13253;
CC P48363; P52553: YKE2; NbExp=5; IntAct=EBI-13239, EBI-13260;
CC -!- MISCELLANEOUS: Present with 6550 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the prefoldin subunit alpha family.
CC {ECO:0000305}.
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DR EMBL; U29137; AAA70038.1; -; Genomic_DNA.
DR EMBL; Z72863; CAA97080.1; -; Genomic_DNA.
DR EMBL; AY557774; AAS56100.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08171.1; -; Genomic_DNA.
DR PIR; S59741; S59741.
DR RefSeq; NP_011592.3; NM_001181207.3.
DR AlphaFoldDB; P48363; -.
DR SMR; P48363; -.
DR BioGRID; 33320; 642.
DR ComplexPortal; CPX-1671; Prefoldin co-chaperone complex.
DR DIP; DIP-6837N; -.
DR IntAct; P48363; 10.
DR MINT; P48363; -.
DR STRING; 4932.YGR078C; -.
DR iPTMnet; P48363; -.
DR MaxQB; P48363; -.
DR PaxDb; P48363; -.
DR PRIDE; P48363; -.
DR EnsemblFungi; YGR078C_mRNA; YGR078C; YGR078C.
DR GeneID; 852969; -.
DR KEGG; sce:YGR078C; -.
DR SGD; S000003310; PAC10.
DR VEuPathDB; FungiDB:YGR078C; -.
DR eggNOG; KOG3313; Eukaryota.
DR GeneTree; ENSGT00390000018904; -.
DR HOGENOM; CLU_083737_0_0_1; -.
DR InParanoid; P48363; -.
DR OMA; DEQHSKY; -.
DR BioCyc; YEAST:G3O-30790-MON; -.
DR PRO; PR:P48363; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P48363; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005844; C:polysome; IDA:SGD.
DR GO; GO:0016272; C:prefoldin complex; IPI:SGD.
DR GO; GO:0015631; F:tubulin binding; IDA:SGD.
DR GO; GO:0007017; P:microtubule-based process; IBA:GO_Central.
DR GO; GO:0006457; P:protein folding; IC:ComplexPortal.
DR GO; GO:0007021; P:tubulin complex assembly; IMP:SGD.
DR Gene3D; 1.10.287.370; -; 1.
DR InterPro; IPR016655; PFD3.
DR InterPro; IPR009053; Prefoldin.
DR InterPro; IPR004127; Prefoldin_subunit_alpha.
DR PANTHER; PTHR12409; PTHR12409; 1.
DR Pfam; PF02996; Prefoldin; 1.
DR PIRSF; PIRSF016396; Prefoldin_subunit_3; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chaperone; Reference proteome.
FT CHAIN 1..199
FT /note="Prefoldin subunit 3"
FT /id="PRO_0000153659"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
SQ SEQUENCE 199 AA; 23115 MW; 884D7449C7AD6233 CRC64;
MDTLFNSTEK NARGIPQAPF IENVNEIIKD PSDFELCFNK FQERLSKYKF MQESKLATIK
QLKTRIPDLE NTLKICQSLR NHSDEGDESD EPILLHYQLN DTLYTKAQVD IPEDRADLKV
GLWLGADVML EYPIDEAIEL LKKKLADSEQ SLTVSTEDVE FLRENITTME VNCARLYNWD
VQRRQDLKQA QEGTKNLKI
