ID   PFDA_METTH              Reviewed;         141 AA.
AC   O27646;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 131.
DE   RecName: Full=Prefoldin subunit alpha;
DE   AltName: Full=GimC subunit alpha;
GN   Name=pfdA; OrderedLocusNames=MTH_1609;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [2]
RP   CHARACTERIZATION.
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=10581246; DOI=10.1093/emboj/18.23.6730;
RA   Leroux M.R., Fandrich M., Klunker D., Siegers K., Lupas A.N., Brown J.R.,
RA   Schiebel E., Dobson C.M., Hartl F.U.;
RT   "MtGimC, a novel archaeal chaperone related to the eukaryotic chaperonin
RT   cofactor GimC/prefoldin.";
RL   EMBO J. 18:6730-6743(1999).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX   PubMed=11106732; DOI=10.1016/s0092-8674(00)00165-3;
RA   Siegert R., Leroux M.R., Scheufler C., Hartl F.U., Moarefi I.;
RT   "Structure of the molecular chaperone prefoldin. Unique interaction of
RT   multiple coiled coil tentacles with unfolded proteins.";
RL   Cell 103:621-632(2000).
CC   -!- FUNCTION: Molecular chaperone capable of stabilizing a range of
CC       proteins. Seems to fulfill an ATP-independent, HSP70-like function in
CC       archaeal de novo protein folding.
CC   -!- SUBUNIT: Heterohexamer of two alpha and four beta subunits.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the prefoldin subunit alpha family.
CC       {ECO:0000305}.
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DR   EMBL; AE000666; AAB86082.1; -; Genomic_DNA.
DR   PIR; H69081; H69081.
DR   RefSeq; WP_010877217.1; NC_000916.1.
DR   PDB; 1FXK; X-ray; 2.30 A; C=7-133.
DR   PDBsum; 1FXK; -.
DR   AlphaFoldDB; O27646; -.
DR   SMR; O27646; -.
DR   STRING; 187420.MTH_1609; -.
DR   EnsemblBacteria; AAB86082; AAB86082; MTH_1609.
DR   GeneID; 1471878; -.
DR   KEGG; mth:MTH_1609; -.
DR   PATRIC; fig|187420.15.peg.1573; -.
DR   HOGENOM; CLU_091867_1_1_2; -.
DR   OMA; ELQNQFM; -.
DR   EvolutionaryTrace; O27646; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016272; C:prefoldin complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006457; P:protein folding; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.287.370; -; 1.
DR   HAMAP; MF_00308; PfdA; 1.
DR   InterPro; IPR011599; PFD_alpha_archaea.
DR   InterPro; IPR009053; Prefoldin.
DR   InterPro; IPR004127; Prefoldin_subunit_alpha.
DR   PANTHER; PTHR12674; PTHR12674; 1.
DR   Pfam; PF02996; Prefoldin; 1.
DR   TIGRFAMs; TIGR00293; TIGR00293; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Cytoplasm; Reference proteome.
FT   CHAIN           1..141
FT                   /note="Prefoldin subunit alpha"
FT                   /id="PRO_0000153679"
FT   HELIX           7..48
FT                   /evidence="ECO:0007829|PDB:1FXK"
FT   STRAND          55..61
FT                   /evidence="ECO:0007829|PDB:1FXK"
FT   STRAND          64..69
FT                   /evidence="ECO:0007829|PDB:1FXK"
FT   STRAND          75..81
FT                   /evidence="ECO:0007829|PDB:1FXK"
FT   STRAND          84..89
FT                   /evidence="ECO:0007829|PDB:1FXK"
FT   HELIX           90..136
FT                   /evidence="ECO:0007829|PDB:1FXK"
SQ   SEQUENCE   141 AA;  15699 MW;  6CA6109863ABD274 CRC64;
     MEDQQRLEEI VNQLNIYQSQ VELIQQQMEA VRATISELEI LEKTLSDIQG KDGSETLVPV
     GAGSFIKAEL KDTSEVIMSV GAGVAIKKNF EDAMESIKSQ KNELESTLQK MGENLRKITD
     IMMKLSPQAE ELLKKVRGSG E