PFES_PSEAE
ID PFES_PSEAE Reviewed; 446 AA.
AC Q04804;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Sensor protein PfeS;
DE EC=2.7.13.3;
GN Name=pfeS; OrderedLocusNames=PA2687;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=8361354; DOI=10.1111/j.1365-2958.1993.tb01654.x;
RA Dean C.R., Poole K.;
RT "Expression of the ferric enterobactin receptor (PfeA) of Pseudomonas
RT aeruginosa: involvement of a two-component regulatory system.";
RL Mol. Microbiol. 8:1095-1103(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Member of the two-component regulatory system PfeR/PfeS. May
CC activate PfeR by phosphorylation.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
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DR EMBL; L07739; AAA25930.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06075.1; -; Genomic_DNA.
DR PIR; A83311; A83311.
DR PIR; S34997; S34997.
DR RefSeq; NP_251377.1; NC_002516.2.
DR RefSeq; WP_003113403.1; NZ_QZGE01000008.1.
DR PDB; 3KYZ; X-ray; 1.50 A; A=28-149.
DR PDBsum; 3KYZ; -.
DR AlphaFoldDB; Q04804; -.
DR SMR; Q04804; -.
DR STRING; 287.DR97_5272; -.
DR PaxDb; Q04804; -.
DR PRIDE; Q04804; -.
DR DNASU; 882765; -.
DR EnsemblBacteria; AAG06075; AAG06075; PA2687.
DR GeneID; 882765; -.
DR KEGG; pae:PA2687; -.
DR PATRIC; fig|208964.12.peg.2812; -.
DR PseudoCAP; PA2687; -.
DR HOGENOM; CLU_000445_89_27_6; -.
DR OMA; KLDWPMS; -.
DR PhylomeDB; Q04804; -.
DR BioCyc; PAER208964:G1FZ6-2727-MON; -.
DR BRENDA; 2.7.13.3; 5087.
DR EvolutionaryTrace; Q04804; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IDA:PseudoCAP.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.450.170; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003660; HAMP_dom.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR031930; HK_sensor.
DR InterPro; IPR038428; HK_sensor_dom_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR Pfam; PF00672; HAMP; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF16750; HK_sensor; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00304; HAMP; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50885; HAMP; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cell inner membrane; Cell membrane; Kinase;
KW Membrane; Nucleotide-binding; Phosphoprotein; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix;
KW Two-component regulatory system.
FT CHAIN 1..446
FT /note="Sensor protein PfeS"
FT /id="PRO_0000074835"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..155
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..446
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 177..233
FT /note="HAMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT DOMAIN 241..446
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 244
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT CONFLICT 304
FT /note="A -> E (in Ref. 1; AAA25930)"
FT /evidence="ECO:0000305"
FT HELIX 41..77
FT /evidence="ECO:0007829|PDB:3KYZ"
FT STRAND 81..84
FT /evidence="ECO:0007829|PDB:3KYZ"
FT STRAND 90..94
FT /evidence="ECO:0007829|PDB:3KYZ"
FT HELIX 98..101
FT /evidence="ECO:0007829|PDB:3KYZ"
FT HELIX 102..104
FT /evidence="ECO:0007829|PDB:3KYZ"
FT TURN 117..120
FT /evidence="ECO:0007829|PDB:3KYZ"
FT STRAND 124..128
FT /evidence="ECO:0007829|PDB:3KYZ"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:3KYZ"
FT STRAND 137..141
FT /evidence="ECO:0007829|PDB:3KYZ"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:3KYZ"
SQ SEQUENCE 446 AA; 50540 MW; D1DA4FED1C222547 CRC64;
MRRHPLLWKL ALLQVGFCLL LTWLIYTWGL SVERSTYFLA PADRHYLADY ARQAEDAWRR
EGAAGAERFR KELSAKEDTW VALVGPHLES LGSTPLSAEE SSHLTFMRKL DWPMSRRLQD
ELPYVSIEFP GHPEQGRLVI QLPERLLPGG LTPWTHLVTH GIVPTLLAAL LGLLLYRHLV
VPLNRLRDRA DALRADELES TPLAAPLAAR RDELGELAQA LEHMAERLRL SLAQQRLLLR
TLSHELRTPL ARLRIAHDSE LPPEQLRQRL DREIGDMQRL LEDTLDLAWM DTERPQLPTE
PVLALSVWEA LRDDACFESG WDPARLPCRL GVDCRVEVHL DSLAQAMENL LRNAIRHSPE
DGTVSLDGER EGDFWHLRLQ DQGPGVAEDQ LERIFLPYQR LDDSAGEGFG LGLAIARRAI
ELQGGRLWAS NGKPGLCLHL WLPAAA
