ID   PFKA3_KOMPG             Reviewed;         351 AA.
AC   C4R7H4; A2SVT8;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=ATP-dependent 6-phosphofructokinase subunit gamma;
DE   AltName: Full=ATP-dependent 6-phosphofructokinase;
DE            Short=ATP-PFK;
DE            Short=Phosphofructokinase 3;
DE   AltName: Full=Phosphohexokinase;
GN   Name=PFK3; OrderedLocusNames=PAS_chr4_0943;
OS   Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=644223;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=GS115 / ATCC 20864, and JC307;
RX   PubMed=17522059; DOI=10.1074/jbc.m611547200;
RA   Tanneberger K., Kirchberger J., Baer J., Schellenberger W., Rothemund S.,
RA   Kamprad M., Otto H., Schoeneberg T., Edelmann A.;
RT   "A novel form of 6-phosphofructokinase. Identification and functional
RT   relevance of a third type of subunit in Pichia pastoris.";
RL   J. Biol. Chem. 282:23687-23697(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GS115 / ATCC 20864;
RX   PubMed=19465926; DOI=10.1038/nbt.1544;
RA   De Schutter K., Lin Y.-C., Tiels P., Van Hecke A., Glinka S.,
RA   Weber-Lehmann J., Rouze P., Van de Peer Y., Callewaert N.;
RT   "Genome sequence of the recombinant protein production host Pichia
RT   pastoris.";
RL   Nat. Biotechnol. 27:561-566(2009).
CC   -!- FUNCTION: Structural subunit of pyrophosphate--fructose 6-phosphate 1-
CC       phosphotransferase. Not required for catalytic activity. Fine-tunes
CC       allosteric regulation of the ATP-PFK by ATP, fructose 2,6-bisphosphate
CC       and AMP (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC       phosphate and glycerone phosphate from D-glucose: step 3/4.
CC   -!- SUBUNIT: Heterododecamer of 4 alpha, 4 beta and 4 gamma chains. The
CC       gamma chain bridges the N-terminal halves of the alpha and beta
CC       subunits (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Decreased growth on nutrient limitation and
CC       reduced cell flocculation compared to wild-type.
CC       {ECO:0000269|PubMed:17522059}.
CC   -!- MISCELLANEOUS: This subunit is only found in some Pichia species.
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DR   EMBL; DQ352840; ABC86235.1; -; Genomic_DNA.
DR   EMBL; DQ374390; ABC86236.1; -; Genomic_DNA.
DR   EMBL; FN392322; CAY71549.1; -; Genomic_DNA.
DR   RefSeq; XP_002493728.1; XM_002493683.1.
DR   AlphaFoldDB; C4R7H4; -.
DR   SMR; C4R7H4; -.
DR   EnsemblFungi; CAY71549; CAY71549; PAS_chr4_0943.
DR   GeneID; 8200752; -.
DR   KEGG; ppa:PAS_chr4_0943; -.
DR   HOGENOM; CLU_790135_0_0_1; -.
DR   UniPathway; UPA00109; UER00182.
DR   Proteomes; UP000000314; Chromosome 4.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.11920; -; 1.
DR   InterPro; IPR038615; PpPFK_gamma_sf.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycolysis; Reference proteome.
FT   CHAIN           1..351
FT                   /note="ATP-dependent 6-phosphofructokinase subunit gamma"
FT                   /id="PRO_0000429726"
SQ   SEQUENCE   351 AA;  40701 MW;  DAE15625A5C95398 CRC64;
     MVTKESIISD LEKENVGPEF GEFLNSLQTD LNSEKPLIEQ VKSQLETHFN LGPETQEFSR
     KNDNAPVDQL LTNYYNNYEV NVLEFVLQMG FCKDLSIPLN VWFVLDMISQ LSTSKQDLPL
     DYYLVLNNSH TGKYSDFVRY LIYEAVGAEI HCFEQGDMPQ QYRSSRWEDK VKGPALANRG
     PIRGNVGAGD RKITFHLLCK KTARMILVGD DRETDFEMSD RSFVTLLLDY YQRVGTTKKI
     DLLLLTNNYD TNMNNKLQQL KILESLNMLK SNCYVLDYQI TADQVTANFN SYVEGIPAFR
     RHEIANFLKK RRTPKNADEL IFKYVGRWNI CYQKKFHQGN ISIHQISGYL D