PFL3_SCHPO
ID PFL3_SCHPO Reviewed; 973 AA.
AC Q874R4;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Putative cell agglutination protein pfl3 {ECO:0000305};
DE AltName: Full=Adhesin pfl3 {ECO:0000303|PubMed:17870620};
DE AltName: Full=Pombe flocculin 3 {ECO:0000303|PubMed:23236291};
DE Flags: Precursor;
GN Name=pfl3 {ECO:0000303|PubMed:23236291};
GN ORFNames=SPBC947.04 {ECO:0000312|PomBase:SPBC947.04};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP DOMAIN, AND REPEATS.
RX PubMed=17870620; DOI=10.1016/j.fgb.2007.08.002;
RA Linder T., Gustafsson C.M.;
RT "Molecular phylogenetics of ascomycotal adhesins--a novel family of
RT putative cell-surface adhesive proteins in fission yeasts.";
RL Fungal Genet. Biol. 45:485-497(2008).
RN [3]
RP FUNCTION.
RX PubMed=23236291; DOI=10.1371/journal.pgen.1003104;
RA Kwon E.J., Laderoute A., Chatfield-Reed K., Vachon L., Karagiannis J.,
RA Chua G.;
RT "Deciphering the transcriptional-regulatory network of flocculation in
RT Schizosaccharomyces pombe.";
RL PLoS Genet. 8:E1003104-E1003104(2012).
CC -!- FUNCTION: May be involved in agglutination during conjugation or other
CC aspects of colony formation (By similarity). Induces flocculation when
CC overexpressed (PubMed:23236291). {ECO:0000250|UniProtKB:O74346,
CC ECO:0000269|PubMed:23236291}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the mam3/map4 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01169}.
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DR EMBL; CU329671; CAA17032.1; -; Genomic_DNA.
DR PIR; T40778; T40778.
DR RefSeq; NP_595272.1; NM_001021179.2.
DR AlphaFoldDB; Q874R4; -.
DR BioGRID; 277751; 10.
DR STRING; 4896.SPBC947.04.1; -.
DR PaxDb; Q874R4; -.
DR EnsemblFungi; SPBC947.04.1; SPBC947.04.1:pep; SPBC947.04.
DR GeneID; 2541237; -.
DR KEGG; spo:SPBC947.04; -.
DR PomBase; SPBC947.04; pfl3.
DR VEuPathDB; FungiDB:SPBC947.04; -.
DR HOGENOM; CLU_305054_0_0_1; -.
DR InParanoid; Q874R4; -.
DR PhylomeDB; Q874R4; -.
DR PRO; PR:Q874R4; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0010339; C:external side of cell wall; TAS:PomBase.
DR GO; GO:0000128; P:flocculation; IMP:PomBase.
DR InterPro; IPR021746; DIPSY.
DR Pfam; PF11763; DIPSY; 1.
DR PROSITE; PS51825; DIPSY; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..973
FT /note="Putative cell agglutination protein pfl3"
FT /id="PRO_0000353811"
FT REPEAT 198..232
FT /note="1"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 233..267
FT /note="2"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 268..302
FT /note="3"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 303..337
FT /note="4"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 338..372
FT /note="5"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 373..407
FT /note="6"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 408..442
FT /note="7"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 443..477
FT /note="8"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 478..512
FT /note="9"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 513..547
FT /note="10"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 548..582
FT /note="11"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 583..617
FT /note="12"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 618..652
FT /note="13"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 653..687
FT /note="14"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 688..722
FT /note="15"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 723..757
FT /note="16"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 758..792
FT /note="17"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 793..828
FT /note="18"
FT /evidence="ECO:0000305|PubMed:17870620"
FT DOMAIN 820..973
FT /note="DIPSY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01169"
FT REGION 198..828
FT /note="18 X 35 AA approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:17870620"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 147
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 184
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 218
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 253
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 352
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 393
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 848
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 973 AA; 100886 MW; 51C5876D19562B85 CRC64;
MSLFPQILLR LLFLAFTLKS TSNAEKHDKV DYKDFVRRDD GDTCLRYTTI YSQGEYETTI
TIPPNSYSNG VSLSNGTLLS TSSPLISTSG QFTSNNVSTT SSQRHNTKTI TFFSGTSDYT
SVYYDTTGTD DVVYEYHPSS ERHKSTNDTW STNLPTNPTT TAIYSTSGSS NITTPYSNRI
TNSNTSVNDI TSKYLSVGTI TLTTISGSDL YTSTFPANGT TSGTVEVVIP TAGTVTETAV
SGSELYTSTF PANGTTSGTV EVVIPTAGTR TVTKISGSKF FTTTTDASGT VSGTVEVVLP
TAGTNTMTVV SGSRFFTSVV SASGTVSGEQ VIVYPTAGMV TETIVSGSEI FNTTYPASGT
RTGTVEVVIP TAGTVTETEI SGSELYTSTF PANGTTSGTV EVVIPTAGTR TVTKISGSKF
FTTTTDASGT VSGTVEVVLP TAGTNTMTVV SGSRFFTSVV SASGTVSGEH IIVEPTAGVV
TETVVSGSVG YTTTYPAHDT VSGTVEVVEP TAGVVTETVV SGSVGYTTAY PAHDTVSGTV
EVVEPTAGVV TETVVSGSVG YTTTYPAHDT VSGTVEVVEP TAGVVTETVV SGSVGYTTTY
PAHDTVSGTV EVVEPTAGVV TETVVSGSVG YTTTYPAHDT VSGTVEVVEP TAGVVTETVV
SGSVGYTTTY PAHDTVSGTV EVVEPTAGVV TETVVSGSVG YTTTYPAHDT VSGTVEVVEP
TAGVVTETVV SGSVGYTTTY PAHDTVSGTV EVVEPTAGVV TETVVSGSVG YTTTYPVQGT
VSGTVMIVEP TAGYVTVTTS LGSSSYATTV ATASGTSTGT VLQVVPSLFP GCNSKCTSSN
AFRILVENDS ISPSYLTYRE SDGFGIASSN PSPAGSEGIF YYDSTLKRVV TCCDERPYYR
TMEGDLAKTY FFIEDEGDGT YKFKLTYGAF SEYLDVKILA DSTFFFKAMD SSSSTILNQK
VRASNVYFKA VPL
