PFL6_SCHPO
ID PFL6_SCHPO Reviewed; 416 AA.
AC P0CU05; Q9P329;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 07-SEP-2016, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=Putative cell agglutination protein pfl6 {ECO:0000305};
DE AltName: Full=Adhesin pfl6 {ECO:0000303|PubMed:17870620};
DE AltName: Full=Pombe flocculin 6 {ECO:0000303|PubMed:23236291};
DE Flags: Precursor;
GN Name=pfl6 {ECO:0000303|PubMed:23236291};
GN ORFNames=SPAC977.07c {ECO:0000312|PomBase:SPAC977.07c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP DOMAIN, AND REPEATS.
RX PubMed=17870620; DOI=10.1016/j.fgb.2007.08.002;
RA Linder T., Gustafsson C.M.;
RT "Molecular phylogenetics of ascomycotal adhesins--a novel family of
RT putative cell-surface adhesive proteins in fission yeasts.";
RL Fungal Genet. Biol. 45:485-497(2008).
RN [3]
RP FUNCTION.
RX PubMed=23236291; DOI=10.1371/journal.pgen.1003104;
RA Kwon E.J., Laderoute A., Chatfield-Reed K., Vachon L., Karagiannis J.,
RA Chua G.;
RT "Deciphering the transcriptional-regulatory network of flocculation in
RT Schizosaccharomyces pombe.";
RL PLoS Genet. 8:E1003104-E1003104(2012).
CC -!- FUNCTION: May be involved in agglutination during conjugation or other
CC aspects of colony formation (By similarity). Induces flocculation when
CC overexpressed (PubMed:23236291). {ECO:0000250|UniProtKB:O74346,
CC ECO:0000269|PubMed:23236291}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000305|PubMed:17870620}.
CC -!- SIMILARITY: Belongs to the mam3/map4 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB69629.1; -; Genomic_DNA.
DR PIR; T50279; T50279.
DR RefSeq; NP_592770.1; NM_001020933.2.
DR RefSeq; NP_592779.1; NM_001018179.1.
DR AlphaFoldDB; P0CU05; -.
DR EnsemblFungi; SPAC977.07c.1; SPAC977.07c.1:pep; SPAC977.07c.
DR EnsemblFungi; SPBC1348.08c.1; SPBC1348.08c.1:pep; SPBC1348.08c.
DR GeneID; 2541886; -.
DR GeneID; 2543047; -.
DR KEGG; spo:SPAC977.07c; -.
DR KEGG; spo:SPBC1348.08c; -.
DR PomBase; SPAC977.07c; pfl6.
DR VEuPathDB; FungiDB:SPAC977.07c; -.
DR VEuPathDB; FungiDB:SPBC1348.08c; -.
DR eggNOG; ENOG502SWCY; Eukaryota.
DR PhylomeDB; P0CU05; -.
DR PRO; PR:P0CU05; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0010339; C:external side of cell wall; TAS:PomBase.
DR GO; GO:0098631; F:cell adhesion mediator activity; ISS:PomBase.
DR GO; GO:0000128; P:flocculation; IMP:PomBase.
DR InterPro; IPR018871; GLEYA_adhesin_domain.
DR InterPro; IPR037524; PA14/GLEYA.
DR Pfam; PF10528; GLEYA; 1.
DR PROSITE; PS51820; PA14; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..416
FT /note="Putative cell agglutination protein pfl6"
FT /id="PRO_0000437229"
FT REPEAT 90..124
FT /note="1"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 125..160
FT /note="2"
FT /evidence="ECO:0000305|PubMed:17870620"
FT DOMAIN 224..390
FT /note="PA14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01164"
FT REGION 90..160
FT /note="2 X 36 AA approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:17870620"
FT CARBOHYD 19
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 344
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 416 AA; 44921 MW; A9F7EC0EE38DE62C CRC64;
MNFFLYFRTI FLIQLYFFNY STFGCSASST SVQSDTTNQV SVSCPKYTTI YTSGTSPDTK
TIYPESTSTK SITTSTQSHS SPVIVVSTVG TVTETTISGS TEYTTTIPAE GITSGTVEIV
EPTAGTVTET ITSGTLPFTT TLAQASGTVS GTVEIVSPKN NPTTVYSGTV ATTETFSSST
VVVIPTAICD GVRGLEYAVY DYTISSSMNE FCYPKNGQTD VFAFNEPAYF GSSDLDQSSP
LFTGVFSSTD DIPEWASSWY LPPYPPQASD MASTYCACKV IVYQFFLRIP ETDTYTLVVN
NVDDVFFGWF GDKAISGWSN NNFDAYSYWH ESPNMGLGTV GMGNFTVGNY PEGYFLPVRF
VVANGAYIGG FDFYFTSDST GPLATTSYSY TKTCTQQFLP FGQGNGGVNG PTEKLS
