PFL9_SCHPO
ID PFL9_SCHPO Reviewed; 326 AA.
AC Q9P7Q2;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Putative cell agglutination protein pfl9 {ECO:0000305};
DE AltName: Full=Adhesin pfl9 {ECO:0000303|PubMed:17870620};
DE AltName: Full=Pombe flocculin 9 {ECO:0000303|PubMed:23236291};
DE Flags: Precursor;
GN Name=pfl9 {ECO:0000303|PubMed:23236291};
GN ORFNames=SPAC186.01 {ECO:0000312|PomBase:SPAC186.01};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP INDUCTION.
RX PubMed=12738880; DOI=10.1073/pnas.1030497100;
RA Samuelsen C.O., Baraznenok V., Khorosjutina O., Spaehr H., Kieselbach T.,
RA Holmberg S., Gustafsson C.M.;
RT "TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally
RT conserved through evolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:6422-6427(2003).
RN [3]
RP DOMAIN, AND REPEATS.
RX PubMed=17870620; DOI=10.1016/j.fgb.2007.08.002;
RA Linder T., Gustafsson C.M.;
RT "Molecular phylogenetics of ascomycotal adhesins--a novel family of
RT putative cell-surface adhesive proteins in fission yeasts.";
RL Fungal Genet. Biol. 45:485-497(2008).
RN [4]
RP FUNCTION.
RX PubMed=23236291; DOI=10.1371/journal.pgen.1003104;
RA Kwon E.J., Laderoute A., Chatfield-Reed K., Vachon L., Karagiannis J.,
RA Chua G.;
RT "Deciphering the transcriptional-regulatory network of flocculation in
RT Schizosaccharomyces pombe.";
RL PLoS Genet. 8:E1003104-E1003104(2012).
CC -!- FUNCTION: May be involved in agglutination during conjugation or other
CC aspects of colony formation (By similarity). Induces flocculation when
CC overexpressed (PubMed:23236291). {ECO:0000250|UniProtKB:O74346,
CC ECO:0000269|PubMed:23236291}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000305}.
CC -!- INDUCTION: Repressed by the srb8 and srb9 transcription factors.
CC {ECO:0000269|PubMed:12738880}.
CC -!- SIMILARITY: Belongs to the mam3/map4 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01169}.
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DR EMBL; CU329670; CAB75865.1; -; Genomic_DNA.
DR PIR; T50128; T50128.
DR RefSeq; NP_595019.1; NM_001020450.2.
DR AlphaFoldDB; Q9P7Q2; -.
DR BioGRID; 278914; 27.
DR STRING; 4896.SPAC186.01.1; -.
DR PaxDb; Q9P7Q2; -.
DR EnsemblFungi; SPAC186.01.1; SPAC186.01.1:pep; SPAC186.01.
DR GeneID; 2542453; -.
DR KEGG; spo:SPAC186.01; -.
DR PomBase; SPAC186.01; pfl9.
DR VEuPathDB; FungiDB:SPAC186.01; -.
DR HOGENOM; CLU_774238_0_0_1; -.
DR PRO; PR:Q9P7Q2; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0010339; C:external side of cell wall; TAS:PomBase.
DR GO; GO:0000128; P:flocculation; IMP:PomBase.
DR InterPro; IPR021746; DIPSY.
DR Pfam; PF11763; DIPSY; 1.
DR PROSITE; PS51825; DIPSY; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..326
FT /note="Putative cell agglutination protein pfl9"
FT /id="PRO_0000353815"
FT REPEAT 103..137
FT /note="1"
FT /evidence="ECO:0000305|PubMed:17870620"
FT REPEAT 138..175
FT /note="2"
FT /evidence="ECO:0000305|PubMed:17870620"
FT DOMAIN 164..326
FT /note="DIPSY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01169"
FT REGION 103..175
FT /note="2 X 36 AA approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:17870620"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 326 AA; 35266 MW; BE7B7751D652346C CRC64;
MNVVKYIIFS FALAPLLLVN ANTYNFTQIQ KRSVNQAVLE SSQDTNSVGG EASSTACPLF
TTIYTNGITP GTTTIYPTSI STSGVSSNNI DETSVSSESI ITSTITTTIT SGSQLYTTTI
TGQNTPVDTV EVVIPTAGTF TTTLTSGSSY PVATTTVRTA SGTQSGEVEV ITPSCGCSPE
NSFHLKIDND KISPSYVYMD PNAPVRTNGA GREGNMFAST NGDNEGLNLF YYDSTIQRVL
TCDCQRPSYT VYIEDPIIGN GFSSAWNLIK NSDGIFTPVE SRNNEPLHFH VDNNGRVWMT
SQEYDTEVSS TDERNFRAND VTLQLY
