ID   PFMAF_PESFW             Reviewed;         456 AA.
AC   W3X7S0;
DT   10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Transcription factor PfmaF {ECO:0000303|PubMed:28517364};
DE   AltName: Full=Conidial pigment biosynthesis cluster protein F {ECO:0000303|PubMed:28517364};
GN   Name=PfmaF {ECO:0000303|PubMed:28517364}; ORFNames=PFICI_07102;
OS   Pestalotiopsis fici (strain W106-1 / CGMCC3.15140).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Xylariomycetidae; Xylariales; Sporocadaceae; Pestalotiopsis.
OX   NCBI_TaxID=1229662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W106-1 / CGMCC3.15140;
RX   PubMed=25623211; DOI=10.1186/s12864-014-1190-9;
RA   Wang X., Zhang X., Liu L., Xiang M., Wang W., Sun X., Che Y., Guo L.,
RA   Liu G., Guo L., Wang C., Yin W.B., Stadler M., Zhang X., Liu X.;
RT   "Genomic and transcriptomic analysis of the endophytic fungus
RT   Pestalotiopsis fici reveals its lifestyle and high potential for synthesis
RT   of natural products.";
RL   BMC Genomics 16:28-28(2015).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=28517364; DOI=10.1111/mmi.13711;
RA   Zhang P., Wang X., Fan A., Zheng Y., Liu X., Wang S., Zou H., Oakley B.R.,
RA   Keller N.P., Yin W.B.;
RT   "A cryptic pigment biosynthetic pathway uncovered by heterologous
RT   expression is essential for conidial development in Pestalotiopsis fici.";
RL   Mol. Microbiol. 105:469-483(2017).
RN   [3]
RP   DISRUPTION PHENOTYPE, AND FUNCTION.
RX   PubMed=31116900; DOI=10.1111/mmi.14281;
RA   Zhang P., Zhou S., Wang G., An Z., Liu X., Li K., Yin W.B.;
RT   "Two transcription factors cooperatively regulate DHN melanin biosynthesis
RT   and development in Pestalotiopsis fici.";
RL   Mol. Microbiol. 112:649-666(2019).
CC   -!- FUNCTION: Transcription factor that positively regulates the expression
CC       of the gene cluster that mediates the biosynthesis of
CC       dihydroxynaphthalene (DHN)-melanin, a bluish-green pigment forming a
CC       dark layer in the conidial wall that protects the conidia from UV
CC       radiations (PubMed:28517364). PfmaF assists PfmaH in promoting melanin
CC       synthesis. PfmaF may function as a broad regulator promoting also other
CC       secondary metabolism as well as fungal development (PubMed:31116900).
CC       {ECO:0000269|PubMed:28517364, ECO:0000269|PubMed:31116900}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect the production of scytalone.
CC       {ECO:0000269|PubMed:28517364, ECO:0000269|PubMed:31116900}.
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DR   EMBL; KI912112; ETS82100.1; -; Genomic_DNA.
DR   RefSeq; XP_007833874.1; XM_007835683.1.
DR   AlphaFoldDB; W3X7S0; -.
DR   SMR; W3X7S0; -.
DR   EnsemblFungi; ETS82100; ETS82100; PFICI_07102.
DR   GeneID; 19272115; -.
DR   KEGG; pfy:PFICI_07102; -.
DR   eggNOG; ENOG502SUCM; Eukaryota.
DR   HOGENOM; CLU_035265_0_0_1; -.
DR   OrthoDB; 1396569at2759; -.
DR   Proteomes; UP000030651; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0042438; P:melanin biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Melanin biosynthesis; Metal-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..456
FT                   /note="Transcription factor PfmaF"
FT                   /id="PRO_0000445354"
FT   DNA_BIND        41..68
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          181..206
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   456 AA;  48490 MW;  7F0241983BE5167F CRC64;
     MSPPHSEHSN VPSDVAAGGG PKKSPPAGTQ KLRAPRMRAS CDGCFLAKVK CSKGKPMCQR
     CMTTGLACKY SPSSRSGKPK ASDNGNILNG APRHAKREGL QFSSPVFEGS QDLMAGPAQF
     MQNNWGSNQQ AMWDLPTHQD PGTNFGSGPL NESLGQDIGI MDGISHDGGV MPFSENLADT
     NPWQSQNPSS SFSQMLPGSS GSMIHGRSQS TDMMISLVAA NFQDTNAQAG PSHGPPLGQM
     AGPSHGPLGQ MAVPLHGPPL GQMAGLSHGP LGQMTSPPNP AHARHHSDPA IGTGHVLSCN
     FDCTHQSLQR LNFMLATSSW PSDMLMTANE NLCDAWRVIM RCPNCMGSGA RRYMALAFLA
     TSMQIQSRRL QAFLDTVKPN HDGVFPTVSN PAEKLNRLGT AITQIDQAYA LLLRSFNNEM
     IEHPGVAQAL ANGVHKSLDA AVSLLLRKRT ELAGTN