ID   PFPC_ENTHI              Reviewed;         101 AA.
AC   Q24825;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Pore-forming peptide ameobapore C;
DE   AltName: Full=EH-APP;
DE   Flags: Precursor;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 25-69.
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RX   PubMed=7715451; DOI=10.1111/j.1365-2958.1994.tb01325.x;
RA   Leippe M., Andrae J., Nickel R., Tannich E., Mueller-Eberhard H.J.;
RT   "Amoebapores, a family of membranolytic peptides from cytoplasmic granules
RT   of Entamoeba histolytica: isolation, primary structure, and pore formation
RT   in bacterial cytoplasmic membranes.";
RL   Mol. Microbiol. 14:895-904(1994).
CC   -!- FUNCTION: Forms pores in the cytoplasmic membrane of host cells. Has
CC       antibacterial activity against M.luteus, no activity against E.coli.
CC       Implicated in the cytolytic activity of the parasite.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic granule.
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DR   EMBL; X76904; CAA54225.1; -; Genomic_DNA.
DR   PIR; S61439; S49145.
DR   AlphaFoldDB; Q24825; -.
DR   SMR; Q24825; -.
DR   TCDB; 1.C.35.1.3; the amoebapore (amoebapore) family.
DR   VEuPathDB; AmoebaDB:EHI5A_228460; -.
DR   VEuPathDB; AmoebaDB:EHI_118270; -.
DR   VEuPathDB; AmoebaDB:KM1_313130; -.
DR   eggNOG; ENOG502RI0B; Eukaryota.
DR   OMA; ICGLIHA; -.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR008138; SapB_2.
DR   InterPro; IPR011001; Saposin-like.
DR   InterPro; IPR008139; SaposinB_dom.
DR   Pfam; PF03489; SapB_2; 1.
DR   SMART; SM00741; SapB; 1.
DR   SUPFAM; SSF47862; SSF47862; 1.
DR   PROSITE; PS50015; SAP_B; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:7715451"
FT   PEPTIDE         25..101
FT                   /note="Pore-forming peptide ameobapore C"
FT                   /id="PRO_0000031670"
FT   DOMAIN          25..101
FT                   /note="Saposin B-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        29..101
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        32..95
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        59..70
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
SQ   SEQUENCE   101 AA;  10855 MW;  28AC72135E73499B CRC64;
     MKLFVLLCVF VLCLASQEKQ QDREIPVLCP VCTSLVGKLI DLVLGGAVDK VTDYLETLCA
     KADGLVETLC TKIVSYGIDK LIEKILEGGS AKLICGLIHA C