ID   PFPN_ENTHI              Reviewed;          97 AA.
AC   Q07831;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Non-pathogenic pore-forming peptide;
DE            Short=APNP;
DE   Flags: Precursor;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 21-34.
RC   STRAIN=SAW 142;
RX   PubMed=8515772; DOI=10.1016/0166-6851(93)90011-l;
RA   Leippe M., Bahr E., Tannich E., Horstmann R.D.;
RT   "Comparison of pore-forming peptides from pathogenic and nonpathogenic
RT   Entamoeba histolytica.";
RL   Mol. Biochem. Parasitol. 59:101-110(1993).
CC   -!- FUNCTION: Forms pores in the membrane of host tissues. Implicated in
CC       the cytolytic activity of the parasite.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
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DR   EMBL; L04984; AAA18632.1; -; Unassigned_DNA.
DR   AlphaFoldDB; Q07831; -.
DR   SMR; Q07831; -.
DR   TCDB; 1.C.35.1.4; the amoebapore (amoebapore) family.
DR   VEuPathDB; AmoebaDB:EHI5A_171330; -.
DR   VEuPathDB; AmoebaDB:EHI7A_127390; -.
DR   VEuPathDB; AmoebaDB:EHI8A_138030; -.
DR   VEuPathDB; AmoebaDB:EHI_159480; -.
DR   VEuPathDB; AmoebaDB:KM1_215020; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008138; SapB_2.
DR   InterPro; IPR011001; Saposin-like.
DR   InterPro; IPR008139; SaposinB_dom.
DR   Pfam; PF03489; SapB_2; 1.
DR   SMART; SM00741; SapB; 1.
DR   SUPFAM; SSF47862; SSF47862; 1.
DR   PROSITE; PS50015; SAP_B; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Disulfide bond; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:8515772"
FT   CHAIN           21..97
FT                   /note="Non-pathogenic pore-forming peptide"
FT                   /id="PRO_0000031671"
FT   DOMAIN          21..97
FT                   /note="Saposin B-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        25..97
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        28..91
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        55..66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
SQ   SEQUENCE   97 AA;  10432 MW;  AE171C58B86EE007 CRC64;
     MKAIVFVLIF AVAFAVTLRQ GPIVCNLCTG LINTLENLLT TKGADKVKDY IDSLCNKASG
     FIATLCTKVL DFGVDKLIQL IEDKVDANAI CAKIHAC