ID   PFR1_TRYBB              Reviewed;         600 AA.
AC   P22225;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=69 kDa paraflagellar rod protein;
DE            Short=69 kDa PFR protein;
DE   AltName: Full=PFR-A/PFR-B;
GN   Name=PFRA;
GN   and
GN   Name=PFRB;
OS   Trypanosoma brucei brucei.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=5702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=STIB 366;
RX   PubMed=2793936; DOI=10.1083/jcb.109.4.1695;
RA   Schlaeppi K., Deflorin J., Seebeck T.;
RT   "The major component of the paraflagellar rod of Trypanosoma brucei is a
RT   helical protein that is encoded by two identical, tandemly linked genes.";
RL   J. Cell Biol. 109:1695-1709(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-8.
RX   PubMed=6088073; DOI=10.1016/0092-8674(84)90552-x;
RA   Parsons M., Nelson R.G., Watkins K.P., Agabian N.;
RT   "Trypanosome mRNAs share a common 5' spliced leader sequence.";
RL   Cell 38:309-316(1984).
CC   -!- FUNCTION: Major component of the paraflagellar rod (PFR). The PFR is a
CC       highly ordered lattices of fibrous proteins that are located inside the
CC       flagellum and assume a fixed orientation with respect to the
CC       microtubular axoneme.
CC   -!- SUBUNIT: Heterodimer of a 69 kDa and a 73 kDa protein.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum. Cytoplasm,
CC       cytoskeleton.
CC   -!- MISCELLANEOUS: Coded by two tandemly repeated genes with identical ORF:
CC       PFRA and PFRB.
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DR   EMBL; X14819; CAA32930.1; -; Genomic_DNA.
DR   EMBL; X14819; CAA32931.1; -; Genomic_DNA.
DR   EMBL; K02195; AAA30212.1; -; mRNA.
DR   PIR; A21440; A21440.
DR   PIR; A33658; A33658.
DR   AlphaFoldDB; P22225; -.
DR   SMR; P22225; -.
DR   OMA; ECMNVTV; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0031514; C:motile cilium; IEA:UniProtKB-SubCell.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   InterPro; IPR007824; Flagellar_rod.
DR   Pfam; PF05149; Flagellar_rod; 1.
PE   2: Evidence at transcript level;
KW   Calmodulin-binding; Cell projection; Cilium; Cytoplasm; Cytoskeleton;
KW   Flagellum.
FT   CHAIN           1..600
FT                   /note="69 kDa paraflagellar rod protein"
FT                   /id="PRO_0000058346"
FT   REGION          335..355
FT                   /note="Calmodulin-binding"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        7
FT                   /note="E -> G (in Ref. 2; AAA30212)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   600 AA;  69597 MW;  CA32D77A1D7E08E1 CRC64;
     MSGKEVEGVV SPADQQQPAV PEVTDITLEA ARKQKIHNLK LKTACLSNEE YVQDLHVSEW
     SETQKQKLQA AHEKAHELLA SVEGGTKWSL TEAYDIKKLM RVCGLEMSVR ELYKPEDKPQ
     FMEIVALKKT MNELKQHHNK TRTVSFTGMI DNAIAKLEKI EDELRRSQLD ASEMAQVPVA
     ALKNIEDTMN VAVVQTALLG NEEQIKAQLA AVEKANEIRN VAIADGEMAI AEEQYYIKAQ
     LLEHLVELVA DKFRIIGQTE DENKSFSKIH EVQKKSFQES ASIKDAKRRL KQHCEDDLRN
     LHDAIQKADL EDAEAMKRFA TQKEKSERFI HENLDKQDEA WRRIQELERV LQRLGTERFE
     EVKRRIEEND REEKRKVEYQ QFLDVCGQHK KLLELSVYNC DLALRCMGML EEIVAEGCSA
     VKSRHDKTND ELSDLRLQVH QEYLEAFRRL YKTLGQLVYK KEKRLEEIDR NIRTTHIQLE
     FAIETFDPNA KLHSDKKKDL YKLRAQVEEE LEMLKDKMAQ ALEMFGPTED ALNQAGIDFV
     HPAEEVESGN MDRRSKMVEY RAHLAKQEEV KIAAEREELK RSKMLQSQQY RGRTMPQITQ