ID   PFS2_CRYNB              Reviewed;         713 AA.
AC   P0CS47; Q55VA1; Q5KKY3;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Polyadenylation factor subunit 2;
GN   Name=PFS2; OrderedLocusNames=CNBC5830;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Required for 3'-end cleavage and polyadenylation of pre-
CC       mRNAs. Also involved in chromosome segregation where it has a role in
CC       chromosome attachment to the mitotic spindle (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
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DR   EMBL; AAEY01000016; EAL21719.1; -; Genomic_DNA.
DR   RefSeq; XP_776366.1; XM_771273.1.
DR   AlphaFoldDB; P0CS47; -.
DR   SMR; P0CS47; -.
DR   PRIDE; P0CS47; -.
DR   EnsemblFungi; EAL21719; EAL21719; CNBC5830.
DR   GeneID; 4935249; -.
DR   KEGG; cnb:CNBC5830; -.
DR   VEuPathDB; FungiDB:CNBC5830; -.
DR   HOGENOM; CLU_000288_77_4_1; -.
DR   Proteomes; UP000001435; Chromosome 3.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR   GO; GO:0031124; P:mRNA 3'-end processing; IEA:InterPro.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR045245; Pfs2-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR22836; PTHR22836; 1.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Chromosome partition; mRNA processing; Nucleus; Repeat; WD repeat.
FT   CHAIN           1..713
FT                   /note="Polyadenylation factor subunit 2"
FT                   /id="PRO_0000410336"
FT   REPEAT          131..170
FT                   /note="WD 1"
FT   REPEAT          173..213
FT                   /note="WD 2"
FT   REPEAT          214..253
FT                   /note="WD 3"
FT   REPEAT          256..295
FT                   /note="WD 4"
FT   REPEAT          298..337
FT                   /note="WD 5"
FT   REPEAT          340..380
FT                   /note="WD 6"
FT   REPEAT          387..426
FT                   /note="WD 7"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          448..474
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          490..713
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        515..531
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        561..591
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        603..675
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   713 AA;  76581 MW;  0F64F1424C96947E CRC64;
     MTAPTVPADQ HGHPLPGPAD PAANDTWRPS RYREPLHPDN EEVLEQAAYQ AAVTRSSGDD
     RKRKIKPRRT VDYQGGVQKW RMLNKLKGVH EFRPAIHPNP SDIVNFLPPV ALRSNPSTSI
     CDYWVHTSIN KERSPTRVVR WTPDARRLLT GNDKGQFTLW NGASFNYESI TQVHDDSIRS
     FTYSHNGQAL VSADKGGTIK YFTPHLTNIH GFQGHREACH DVSWSPNDER FVTCGDDGLV
     KIWSYREAKE ERSLSGHGWD VRCVDWHPTK GLIVSGSKDM LVKFWDPRTG KDLSTLHSSK
     STINTCRWSP DGHLVATAGQ DSVIRLFDIR TFRELEVLKG HEKEVNCIEW HPIHHSLLVS
     GDALGTINYF SLLSPTPSTP ITTLSAAHED AVFSLSFHPL GHILCSGSKD FTARFWCRAR
     PPGGQEFDKW HLTEEGAAQK ELERITKREW GTNALPANAA GGGGGGGGGD KQQVALPGLS
     NLVAAVNSVK TGPTTTGGGP PGLPGLGAPN VHAGTPPSRV STPSSMGPPG AGAQGQAQGG
     QFPRGRAALP SQNDMLRHNH GPRGGFADRD RNGGGDRGGM DRDRDSRGGR QDPRGNQMYG
     RGPGGPPPGP PPGQGGYNYP PAPPNYPPYP PSSYPPPPNN QPGYPPAPNY AMPPGPGAPP
     QSYPYNRPPQ GPPQNNNPGG QGNYGASASG GYGQYGGGGG GGGGGGYGRD GRR