ID   PFS2_CRYNJ              Reviewed;         712 AA.
AC   P0CS46; Q55VA1; Q5KKY3;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Polyadenylation factor subunit 2;
GN   Name=PFS2; OrderedLocusNames=CNC01430;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Required for 3'-end cleavage and polyadenylation of pre-
CC       mRNAs. Also involved in chromosome segregation where it has a role in
CC       chromosome attachment to the mitotic spindle (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017343; AAW42164.1; -; Genomic_DNA.
DR   RefSeq; XP_569471.1; XM_569471.1.
DR   AlphaFoldDB; P0CS46; -.
DR   SMR; P0CS46; -.
DR   STRING; 5207.AAW42164; -.
DR   PaxDb; P0CS46; -.
DR   EnsemblFungi; AAW42164; AAW42164; CNC01430.
DR   GeneID; 3256507; -.
DR   KEGG; cne:CNC01430; -.
DR   VEuPathDB; FungiDB:CNC01430; -.
DR   eggNOG; KOG0284; Eukaryota.
DR   HOGENOM; CLU_000288_77_4_1; -.
DR   InParanoid; P0CS46; -.
DR   OMA; WSPNGNM; -.
DR   OrthoDB; 604979at2759; -.
DR   Proteomes; UP000002149; Chromosome 3.
DR   GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IBA:GO_Central.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR   GO; GO:0006378; P:mRNA polyadenylation; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 3.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR045245; Pfs2-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR22836; PTHR22836; 1.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   Pfam; PF00400; WD40; 3.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Chromosome partition; mRNA processing; Nucleus; Reference proteome; Repeat;
KW   WD repeat.
FT   CHAIN           1..712
FT                   /note="Polyadenylation factor subunit 2"
FT                   /id="PRO_0000238500"
FT   REPEAT          131..170
FT                   /note="WD 1"
FT   REPEAT          173..213
FT                   /note="WD 2"
FT   REPEAT          214..253
FT                   /note="WD 3"
FT   REPEAT          256..295
FT                   /note="WD 4"
FT   REPEAT          298..337
FT                   /note="WD 5"
FT   REPEAT          340..380
FT                   /note="WD 6"
FT   REPEAT          387..426
FT                   /note="WD 7"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          446..473
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          489..712
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        514..530
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        560..590
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        602..674
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   712 AA;  76530 MW;  27498952BBA2D615 CRC64;
     MTAPTVPADQ HGHPLPGPAD PAANDTWRPS RYREPLHPDN EEVLEQAAYQ AAVTRSSGDD
     RKRKIKPRRT VDYQGGVQKW RMLNKLKGVH EFRPAIHPNP SDIVNFLPPV ALRSNPSTSI
     CDYWVHTSIN KERSPTRVVR WTPDARRLLT GNDKGQFTLW NGASFNYESI TQVHDDSIRS
     FTYSHNGQAL VSADKGGTIK YFTPHLTNIH GFQGHREACH DVSWSPNDER FVTCGDDGLV
     KIWSYREAKE ERSLSGHGWD VRCVDWHPTK GLIVSGSKDM LVKFWDPRTG KDLSTLHSSK
     STINTCRWSP DGHLVATAGQ DSVIRLFDIR TFRELEVLKG HEKEVNCIEW HPIHHSLLVS
     GDALGTINYF SLLSPTPSTP ITTLSAAHED AVFSLSFHPL GHILCSGSKD FTARFWCRAR
     PPGGQEFDKW HLTEEGAAQK ELERITKREW GTNAPPANAA GGGGGGGGDK QQVALPGLSN
     LVAAVNSVKT GPTTTGGGPS GLPGLGAPNV HAGTPPSRVS TPSSMGPPGP GAQGQAQGGQ
     FPRGRSALPS QNDMLRHNHG SRGGFADRDR NGGGDRGGMD RDRDSRGGRQ DPRGNQMYGR
     GPGGPPPGPP PGQGGYNYPP APPNYPPYPP SSYPPPPNNQ PGYPPAPNYA MPPGPGAPPQ
     SYPYNRPPQG PPQNNNPGGQ GNYGASASGG YGQYGGGGGG GGGGGYGRDG RR