ID   PFS2_KLULA              Reviewed;         452 AA.
AC   Q6CP71;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Polyadenylation factor subunit 2;
GN   Name=PFS2; OrderedLocusNames=KLLA0E07073g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Required for 3'-end cleavage and polyadenylation of pre-
CC       mRNAs. Also involved in chromosome segregation where it has a role in
CC       chromosome attachment to the mitotic spindle (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
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DR   EMBL; CR382125; CAG99355.1; -; Genomic_DNA.
DR   RefSeq; XP_454268.1; XM_454268.1.
DR   AlphaFoldDB; Q6CP71; -.
DR   SMR; Q6CP71; -.
DR   STRING; 28985.XP_454268.1; -.
DR   EnsemblFungi; CAG99355; CAG99355; KLLA0_E07085g.
DR   GeneID; 2893820; -.
DR   KEGG; kla:KLLA0_E07085g; -.
DR   eggNOG; KOG0284; Eukaryota.
DR   HOGENOM; CLU_000288_77_0_1; -.
DR   InParanoid; Q6CP71; -.
DR   OMA; HHWDVKS; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IEA:EnsemblFungi.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR   GO; GO:0006378; P:mRNA polyadenylation; IEA:EnsemblFungi.
DR   GO; GO:0098789; P:pre-mRNA cleavage required for polyadenylation; IEA:EnsemblFungi.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR045245; Pfs2-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR22836; PTHR22836; 1.
DR   Pfam; PF00400; WD40; 4.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 4.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Chromosome partition; mRNA processing; Nucleus; Reference proteome; Repeat;
KW   WD repeat.
FT   CHAIN           1..452
FT                   /note="Polyadenylation factor subunit 2"
FT                   /id="PRO_0000238504"
FT   REPEAT          79..118
FT                   /note="WD 1"
FT   REPEAT          121..160
FT                   /note="WD 2"
FT   REPEAT          163..202
FT                   /note="WD 3"
FT   REPEAT          205..244
FT                   /note="WD 4"
FT   REPEAT          247..287
FT                   /note="WD 5"
FT   REPEAT          290..330
FT                   /note="WD 6"
FT   REPEAT          338..377
FT                   /note="WD 7"
FT   REGION          409..452
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   452 AA;  50882 MW;  ED4E01F06B651F1E CRC64;
     MDPNTEGTTT KKYTSQRRTI DISASYDRLY FLKKHGQENS SYIEAETSYN AHILPPDAYG
     FHGHAINTAT KFTHLSSNKV KHVIPALTWT PEGRRLVVAT YNGEFSLWSG SSFNFESIMQ
     AHDSAVTVMT YSHTGDWMVS GSADGELKIW QPNFNMVKVM DQAHMECVRE ISFSPTDQKF
     VSCSDDNVLK IWNFSNGQQE RVLSGHHWDV KSCDWHPKMG LIVSGSKDNL IKFWDPRSGS
     CVSTMLGFKH TIISTKFQPK QGNLLSVISK DKTCKVYDIR QQAKELFSVR DDVDYMTLQW
     HPIDETVFTV GCYDGSIKHF DLSQENQPNK PTHNIPYAHE KCVTSLAYSP IGHIMASASK
     DRTIRFWTRS RAVDPNAFDD PTYNNEKVNA WYFGINNNIN AVRNKTEHGV ALPPSEDSNN
     NENGDGSRSV EPERPSVSTL PGLGSGLPGL SF