ID   PFS2_SCHPO              Reviewed;         509 AA.
AC   Q9UTN4; Q09876;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 140.
DE   RecName: Full=Polyadenylation factor subunit 2;
GN   Name=pfs2; ORFNames=SPAC12G12.14c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=15743824; DOI=10.1128/mcb.25.6.2288-2296.2005;
RA   Wang S.-W., Asakawa K., Win T.Z., Toda T., Norbury C.J.;
RT   "Inactivation of the pre-mRNA cleavage and polyadenylation factor Pfs2 in
RT   fission yeast causes lethal cell cycle defects.";
RL   Mol. Cell. Biol. 25:2288-2296(2005).
CC   -!- FUNCTION: Required for 3'-end cleavage and polyadenylation of pre-
CC       mRNAs. Also involved in chromosome segregation where it has a role in
CC       chromosome attachment to the mitotic spindle.
CC       {ECO:0000269|PubMed:15743824}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15743824}.
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DR   EMBL; CU329670; CAB55873.1; -; Genomic_DNA.
DR   PIR; T37587; T37587.
DR   RefSeq; NP_592884.1; NM_001018284.2.
DR   AlphaFoldDB; Q9UTN4; -.
DR   SMR; Q9UTN4; -.
DR   BioGRID; 279522; 11.
DR   STRING; 4896.SPAC12G12.14c.1; -.
DR   SwissPalm; Q9UTN4; -.
DR   MaxQB; Q9UTN4; -.
DR   PaxDb; Q9UTN4; -.
DR   EnsemblFungi; SPAC12G12.14c.1; SPAC12G12.14c.1:pep; SPAC12G12.14c.
DR   GeneID; 2543089; -.
DR   KEGG; spo:SPAC12G12.14c; -.
DR   PomBase; SPAC12G12.14c; pfs2.
DR   VEuPathDB; FungiDB:SPAC12G12.14c; -.
DR   eggNOG; KOG0284; Eukaryota.
DR   HOGENOM; CLU_000288_77_1_1; -.
DR   InParanoid; Q9UTN4; -.
DR   OMA; HHWDVKS; -.
DR   PhylomeDB; Q9UTN4; -.
DR   Reactome; R-SPO-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR   Reactome; R-SPO-72187; mRNA 3'-end processing.
DR   Reactome; R-SPO-77595; Processing of Intronless Pre-mRNAs.
DR   PRO; PR:Q9UTN4; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0000785; C:chromatin; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005847; C:mRNA cleavage and polyadenylation specificity factor complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IDA:PomBase.
DR   GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR   GO; GO:0031124; P:mRNA 3'-end processing; IMP:PomBase.
DR   GO; GO:0006379; P:mRNA cleavage; ISO:PomBase.
DR   GO; GO:0006378; P:mRNA polyadenylation; ISO:PomBase.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR045245; Pfs2-like.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   PANTHER; PTHR22836; PTHR22836; 1.
DR   Pfam; PF00400; WD40; 6.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS50082; WD_REPEATS_2; 6.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   4: Predicted;
KW   Chromosome partition; mRNA processing; Nucleus; Reference proteome; Repeat;
KW   WD repeat.
FT   CHAIN           1..509
FT                   /note="Polyadenylation factor subunit 2"
FT                   /id="PRO_0000051491"
FT   REPEAT          72..109
FT                   /note="WD 1"
FT   REPEAT          113..151
FT                   /note="WD 2"
FT   REPEAT          156..192
FT                   /note="WD 3"
FT   REPEAT          196..234
FT                   /note="WD 4"
FT   REPEAT          238..278
FT                   /note="WD 5"
FT   REPEAT          282..321
FT                   /note="WD 6"
FT   REPEAT          350..388
FT                   /note="WD 7"
FT   REGION          385..409
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          480..509
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        386..409
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   509 AA;  57654 MW;  77A549D28DDB5B2B CRC64;
     MERAENARII QKPMTRRTVD YGSGLSKYIV NRHLRSNRYH IHVPRPNPNQ IINLYPPYEY
     KYNNTSSLCT KYIHTSANKA RHVINVVRWT PDGRRLLTGS STGEFTLWNG LTFNFELINQ
     SHDYAVRCAE WSTDGRWLIS GDGGGMVKYF EPNLNNVKIV QAHEMEVRDV AFSPNDSKFV
     TASDDGSLKV WNFHMSTEEL KLTGHGWDVK TVDWHPSKGL LASGSKDNLV KFWDPRTGTC
     IATLHGHKNT IMQASFQKNF GSNYLATVSR DSTCRVFDLR AMKDVRVLRG HEKDVNCVTW
     HPLYPNLLTT GGSDGSVNHY SLDEPPLLSQ QKYHEKHPNV TLSASSYLLY PTAEIPFAHD
     LGIWSMQYHP LGHLLCTGSN DKTTRFWSRS RPDDKESTMD RHHLGEEQSE AMLSQRKAAI
     EEDDNYEPDE NPLTETLANA HNPQFSGVLN LPGLGTMPSF PSPYQHGQPQ IPGMLHASLS
     NSYAEPSTQN SFIPGLTSKS QDGYPQNYR