ID   PFS47_PLABA             Reviewed;         431 AA.
AC   Q9BMP8; Q4YSU5;
DT   18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Female gametocyte surface protein P47;
DE   Flags: Precursor;
GN   Name=PB47; ORFNames=PB001526.02.0;
OS   Plasmodium berghei (strain Anka).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX   NCBI_TaxID=5823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ANKA;
RX   PubMed=11163248; DOI=10.1016/s0092-8674(01)00199-4;
RA   van Dijk M.R., Janse C.J., Thompson J., Waters A.P., Braks J.A.,
RA   Dodemont H.J., Stunnenberg H.G., van Gemert G.J., Sauerwein R.W., Eling W.;
RT   "A central role for P48/45 in malaria parasite male gamete fertility.";
RL   Cell 104:153-164(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ANKA;
RX   PubMed=15637271; DOI=10.1126/science.1103717;
RA   Hall N., Karras M., Raine J.D., Carlton J.M., Kooij T.W.A., Berriman M.,
RA   Florens L., Janssen C.S., Pain A., Christophides G.K., James K.,
RA   Rutherford K., Harris B., Harris D., Churcher C.M., Quail M.A., Ormond D.,
RA   Doggett J., Trueman H.E., Mendoza J., Bidwell S.L., Rajandream M.A.,
RA   Carucci D.J., Yates J.R. III, Kafatos F.C., Janse C.J., Barrell B.G.,
RA   Turner C.M.R., Waters A.P., Sinden R.S.;
RT   "A comprehensive survey of the Plasmodium life cycle by genomic,
RT   transcriptomic, and proteomic analyses.";
RL   Science 307:82-86(2005).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RC   STRAIN=ANKA;
RX   PubMed=20386715; DOI=10.1371/journal.ppat.1000853;
RA   van Dijk M.R., van Schaijk B.C., Khan S.M., van Dooren M.W., Ramesar J.,
RA   Kaczanowski S., van Gemert G.J., Kroeze H., Stunnenberg H.G., Eling W.M.,
RA   Sauerwein R.W., Waters A.P., Janse C.J.;
RT   "Three members of the 6-cys protein family of Plasmodium play a role in
RT   gamete fertility.";
RL   PLoS Pathog. 6:E1000853-E1000853(2010).
CC   -!- FUNCTION: Required for female fertility. {ECO:0000269|PubMed:20386715}.
CC   -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000250}. Cell membrane
CC       {ECO:0000250}; Lipid-anchor, GPI-anchor {ECO:0000250}.
CC       Note=Specifically present on the surface of female gametocytes.
CC       {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed only in female gametocytes.
CC       {ECO:0000269|PubMed:20386715}.
CC   -!- DISRUPTION PHENOTYPE: Strong reduction of female fertility, while males
CC       remain unaffected. Gametes are unable to either recognize or attach to
CC       each other. {ECO:0000269|PubMed:20386715}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAH98912.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AF314253; AAG59814.1; -; Genomic_DNA.
DR   EMBL; CAAI01002547; CAH98912.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q9BMP8; -.
DR   STRING; 5821.PBANKA_135970; -.
DR   VEuPathDB; PlasmoDB:PBANKA_1359700; -.
DR   eggNOG; ENOG502TMWJ; Eukaryota.
DR   HOGENOM; CLU_052271_0_0_1; -.
DR   OMA; VRCVCEH; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.2860; -; 2.
DR   InterPro; IPR010884; 6_CYS_dom.
DR   InterPro; IPR038160; 6_CYS_dom_sf.
DR   Pfam; PF07422; s48_45; 2.
DR   SMART; SM00970; s48_45; 2.
DR   PROSITE; PS51701; 6_CYS; 2.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW   Malaria; Membrane; Repeat; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..409
FT                   /note="Female gametocyte surface protein P47"
FT                   /id="PRO_0000423581"
FT   PROPEP          410..431
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000423582"
FT   DOMAIN          26..178
FT                   /note="6-Cys 1"
FT   DOMAIN          278..409
FT                   /note="6-Cys 2"
FT   LIPID           409
FT                   /note="GPI-anchor amidated serine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        45
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        239
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        30..55
FT                   /evidence="ECO:0000250"
FT   DISULFID        70..145
FT                   /evidence="ECO:0000250"
FT   DISULFID        88..143
FT                   /evidence="ECO:0000250"
FT   DISULFID        282..309
FT                   /evidence="ECO:0000250"
FT   DISULFID        326..395
FT                   /evidence="ECO:0000250"
FT   DISULFID        335..393
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   431 AA;  48929 MW;  AA457479FDA91CFE CRC64;
     MKGFTGASII VFYLIKGYLS YIIFPNGYVC DFKFNPLVNV LPSINTTGDI EEVGCTINNP
     SLSDYIALVC PKKNYNDYEH MEKVPSKCFS SNLYSPYKSE DSAHKLEELK IPEKYSISKD
     FSDFDLNIIL IPSLYNIDKT IYCRCDNSKT KRELIKNDGE NIKLQGKLGL VKIILNNQQN
     SPQNIYHITR STQVGSLDNK VIELKEGEIV HLKYDGKTRT NFNCKEIINM KISIPLDYNL
     SMRMPTVFLK DINCKFHLIF SNVGGIANIV FKAKKTENID GCDFTVPKGK GLYKNGFALS
     EINNDEEICT VHIGRGQNSN AAGLKCPYNL TPAHCFKHVL YEKKYKNGNN VFQTFLLDDV
     LRTVDIEYYY NAKLSAHIVG IPTIPEKSET VRCVCEHDGK KGIMELKISS SKNIFISFIL
     LSIIVSIFYL F