PFS47_PLAF7
ID PFS47_PLAF7 Reviewed; 439 AA.
AC Q8IDN0;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=Female gametocyte surface protein P47;
DE Flags: Precursor;
GN Name=PFS47; Synonyms=PF47; ORFNames=PF13_0248;
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7;
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11163248; DOI=10.1016/s0092-8674(01)00199-4;
RA van Dijk M.R., Janse C.J., Thompson J., Waters A.P., Braks J.A.,
RA Dodemont H.J., Stunnenberg H.G., van Gemert G.J., Sauerwein R.W., Eling W.;
RT "A central role for P48/45 in malaria parasite male gamete fertility.";
RL Cell 104:153-164(2001).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16824624; DOI=10.1016/j.molbiopara.2006.05.015;
RA van Schaijk B.C., van Dijk M.R., van de Vegte-Bolmer M., van Gemert G.J.,
RA van Dooren M.W., Eksi S., Roeffen W.F., Janse C.J., Waters A.P.,
RA Sauerwein R.W.;
RT "Pfs47, paralog of the male fertility factor Pfs48/45, is a female specific
RT surface protein in Plasmodium falciparum.";
RL Mol. Biochem. Parasitol. 149:216-222(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23661646; DOI=10.1126/science.1235264;
RA Molina-Cruz A., Garver L.S., Alabaster A., Bangiolo L., Haile A.,
RA Winikor J., Ortega C., van Schaijk B.C., Sauerwein R.W., Taylor-Salmon E.,
RA Barillas-Mury C.;
RT "The human malaria parasite Pfs47 gene mediates evasion of the mosquito
RT immune system.";
RL Science 340:984-987(2013).
CC -!- FUNCTION: Mediates evasion of the mosquito innate immune response by
CC suppressing midgut nitration responses that are critical to activate
CC the mosquito complement system. It is unclear whether the protein has a
CC role in passive evasion by making the parasite invisible to the
CC mosquito immune system, acts as a decoy to prevent recognition of
CC another surface protein or anchors the decoy protein. Not required for
CC female fertility. {ECO:0000269|PubMed:16824624,
CC ECO:0000269|PubMed:23661646}.
CC -!- SUBCELLULAR LOCATION: Cell surface {ECO:0000269|PubMed:16824624}. Cell
CC membrane {ECO:0000305|PubMed:16824624}; Lipid-anchor, GPI-anchor
CC {ECO:0000305|PubMed:16824624}. Note=Specifically present on the surface
CC of female gametocytes. {ECO:0000269|PubMed:16824624}.
CC -!- DEVELOPMENTAL STAGE: Expressed only in female gametocytes following
CC emergence from red blood cells. {ECO:0000269|PubMed:16824624}.
CC -!- DISRUPTION PHENOTYPE: Reduced parasite survival in the mosquito.
CC Parasites produce normal numbers of oocysts when included in the blood
CC meal of the mosquito vector. {ECO:0000269|PubMed:16824624,
CC ECO:0000269|PubMed:23661646}.
CC -!- MISCELLANEOUS: Exhibits a high rate of non-synonymous polymorphism is
CC different strains, suggesting that it adapts to different Anopheles
CC vector species. {ECO:0000305|PubMed:23661646}.
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DR EMBL; AL844509; CAD52591.1; -; Genomic_DNA.
DR RefSeq; XP_001350182.1; XM_001350146.1.
DR AlphaFoldDB; Q8IDN0; -.
DR STRING; 5833.PF13_0248; -.
DR PRIDE; Q8IDN0; -.
DR EnsemblProtists; CAD52591; CAD52591; PF3D7_1346800.
DR GeneID; 814213; -.
DR KEGG; pfa:PF3D7_1346800; -.
DR VEuPathDB; PlasmoDB:PF3D7_1346800; -.
DR HOGENOM; CLU_052271_0_0_1; -.
DR InParanoid; Q8IDN0; -.
DR OMA; VRCVCEH; -.
DR PhylomeDB; Q8IDN0; -.
DR Proteomes; UP000001450; Chromosome 13.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; TAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042783; P:evasion of host immune response; IMP:GeneDB.
DR GO; GO:0044068; P:modulation by symbiont of host cellular process; IMP:GeneDB.
DR GO; GO:0052170; P:suppression by symbiont of host innate immune response; IDA:GO_Central.
DR Gene3D; 2.60.40.2860; -; 2.
DR InterPro; IPR010884; 6_CYS_dom.
DR InterPro; IPR038160; 6_CYS_dom_sf.
DR Pfam; PF07422; s48_45; 2.
DR SMART; SM00970; s48_45; 2.
DR PROSITE; PS51701; 6_CYS; 2.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW Malaria; Membrane; Reference proteome; Repeat; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..415
FT /note="Female gametocyte surface protein P47"
FT /id="PRO_0000423579"
FT PROPEP 416..439
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000423580"
FT DOMAIN 32..182
FT /note="6-Cys 1"
FT DOMAIN 283..415
FT /note="6-Cys 2"
FT LIPID 415
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 324
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 36..62
FT /evidence="ECO:0000250"
FT DISULFID 77..152
FT /evidence="ECO:0000250"
FT DISULFID 95..150
FT /evidence="ECO:0000250"
FT DISULFID 287..315
FT /evidence="ECO:0000250"
FT DISULFID 332..400
FT /evidence="ECO:0000250"
FT DISULFID 341..398
FT /evidence="ECO:0000250"
SQ SEQUENCE 439 AA; 50824 MW; F9F0DA79A9806012 CRC64;
MCMGRMISII NIILFYFFLW VKKSISELLS STQYVCDFYF NPLTNVKPTV VGSSEIYEEV
GCTINNPTLG DHIVLICPKK NNGDFSNIEI VPTNCFESHL YSAYKNDSSA YHLEKLDIDK
KYAINSSFSD FYLKILVIPN EYKSHKTIYC RCDNSKTEKN IPGQDKILKG KLGLVKIILR
NQYNNIIELE KTKPIIHNKK DTYKYDIKLK ESDILMFYMK EETIVESGNC EEILNTKINL
LSNNNVVIKM PSIFINNINC MLSSQDQNNE KNYINLKADK TKHIDGCDFT KPKGKGIYKN
GFIINDIPNE EERICTVHLW NKKNQTIAGI KCPYKLIPPY CFKHVLYEKE IDSQKTYKTF
LLSDVLDTPN IEYYGNNKEG MYMLALPTKP EKTNKIRCIC EQGGKKAVME LHIASTSTKY
ISMFLIFFLI VIFYMYVSI
