PG05_MYCTU
ID PG05_MYCTU Reviewed; 591 AA.
AC Q6MX50; I6Y3F7; L0T693;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=PE-PGRS family protein PE_PGRS5 {ECO:0000305};
GN Name=PE_PGRS5 {ECO:0000312|EMBL:CCP43027.1};
GN OrderedLocusNames=Rv0297 {ECO:0000312|EMBL:CCP43027.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2] {ECO:0007744|PubMed:21969609}
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [3]
RP FUNCTION, INTERACTION WITH TLR4, SUBCELLULAR LOCATION, AND DOMAIN.
RC STRAIN=H37Rv;
RX PubMed=29921671; DOI=10.1128/mbio.01017-18;
RA Grover S., Sharma T., Singh Y., Kohli S., P M., Singh A., Semmler T.,
RA Wieler L.H., Tedin K., Ehtesham N.Z., Hasnain S.E.;
RT "The PGRS domain of Mycobacterium tuberculosis PE_PGRS protein Rv0297 is
RT involved in endoplasmic reticulum stress-mediated apoptosis through Toll-
RT like receptor 4.";
RL MBio 9:E01017-E01017(2018).
CC -!- FUNCTION: Involved in endoplasmic reticulum (ER) stress-mediated
CC apoptosis through human Toll-like receptor 4 (TLR4) signaling pathway.
CC Localizes to the host ER, leading to ER stress, disruption of
CC intracellular Ca(2+) homeostasis and increase of nitric oxide (NO) and
CC reactive oxygen species (ROS) levels. Stress response results in
CC caspase-8 activation and apoptosis of macrophage cells. Apoptosis may
CC lead to dissemination of the bacteria, thereby spreading the disease.
CC {ECO:0000269|PubMed:29921671}.
CC -!- SUBUNIT: Interacts with human TLR4. {ECO:0000269|PubMed:29921671}.
CC -!- SUBCELLULAR LOCATION: Host endoplasmic reticulum
CC {ECO:0000269|PubMed:29921671}.
CC -!- DOMAIN: The PGRS region is important for localization and function.
CC {ECO:0000269|PubMed:29921671}.
CC -!- SIMILARITY: Belongs to the mycobacterial PE family. PGRS subfamily.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP43027.1; -; Genomic_DNA.
DR RefSeq; WP_010886074.1; NZ_KK339370.1.
DR RefSeq; YP_177713.1; NC_000962.3.
DR AlphaFoldDB; Q6MX50; -.
DR SMR; Q6MX50; -.
DR STRING; 83332.Rv0297; -.
DR PaxDb; Q6MX50; -.
DR DNASU; 885981; -.
DR GeneID; 885981; -.
DR KEGG; mtu:Rv0297; -.
DR PATRIC; fig|83332.111.peg.333; -.
DR TubercuList; Rv0297; -.
DR eggNOG; COG3391; Bacteria.
DR OMA; DEYIKGY; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0044165; C:host cell endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR InterPro; IPR000084; PE-PGRS_N.
DR Pfam; PF00934; PE; 1.
PE 1: Evidence at protein level;
KW Host endoplasmic reticulum; Reference proteome; Virulence.
FT CHAIN 1..591
FT /note="PE-PGRS family protein PE_PGRS5"
FT /id="PRO_0000445087"
FT DOMAIN 1..93
FT /note="PE"
FT /evidence="ECO:0000255"
FT REGION 94..591
FT /note="PGRS"
FT /evidence="ECO:0000305|PubMed:29921671"
FT REGION 303..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 468..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 539..591
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 325..342
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 591 AA; 49058 MW; 4E4675EDD4297F5A CRC64;
MSFVIAQPEM IAAAAGELAS IRSAINAANA AAAAQTTGVM SAAADEVSTA VAALFSSHAQ
AYQAASAQAA AFHAQVVRTL TVDAGAYASA EAANAGPNML AAVNAPAQAL LGRPLIGNGA
NGAPGTGQAG GDGGLLFGNG GNGGSGAPGQ AGGAGGAAGF FGNGGNGGDG GAGANGGAGG
TAGWFFGFGG NGGAGGIGVA GINGGLGGAG GDGGNAGFFG NGGNGGMGGA GAAGVNAVNP
GLATPVTPAA NGGNGLNLVG VPGTAGGGAD GANGSAIGQA GGAGGDGGNA STSGGIGIAQ
TGGAGGAGGA GGDGAPGGNG GNGGSVEHTG ATGSSASGGN GATGGNGGVG APGGAGGNGG
HVSGGSVNTA GAGGKGGNGG TGGAGGPGGH GGSVLSGPVG DSGNGGAGGD GGAGVSATDI
AGTGGRGGNG GHGGLWIGNG GDGGAGGVGG VGGAGAAGAI GGHGGDGGSV NTPIGGSEAG
DGGKGGLGGD GGGRGIFGQF GAGGAGGAGG VGGAGGAGGT GGGGGNGGAI FNAGTPGAAG
TGGDGGVGGT GAAGGKGGAG GSGGVNGATG ADGAKGLDGA TGGKGNNGNP G
