PG18_MYCTU
ID PG18_MYCTU Reviewed; 457 AA.
AC Q79FU0; I6XA99; L0T5C9;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=PE-PGRS family protein PE_PGRS18 {ECO:0000305};
GN Name=PE_PGRS18 {ECO:0000312|EMBL:CCP43730.1};
GN OrderedLocusNames=Rv0980c {ECO:0000312|EMBL:CCP43730.1};
GN ORFNames=LH57_05345 {ECO:0000312|EMBL:AIR13715.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=16586367; DOI=10.1086/502631;
RA Jain S.K., Paul-Satyaseela M., Lamichhane G., Kim K.S., Bishai W.R.;
RT "Mycobacterium tuberculosis invasion and traversal across an in vitro human
RT blood-brain barrier as a pathogenic mechanism for central nervous system
RT tuberculosis.";
RL J. Infect. Dis. 193:1287-1295(2006).
RN [4]
RP FUNCTION, EXPRESSION IN M.SMEGMATIS, AND SUBCELLULAR LOCATION.
RX PubMed=27987050; DOI=10.1007/s10495-016-1336-0;
RA Yang W., Deng W., Zeng J., Ren S., Ali M.K., Gu Y., Li Y., Xie J.;
RT "Mycobacterium tuberculosis PE_PGRS18 enhances the intracellular survival
RT of M. smegmatis via altering host macrophage cytokine profiling and
RT attenuating the cell apoptosis.";
RL Apoptosis 22:502-509(2017).
CC -!- FUNCTION: Enhances mycobacterial intracellular survival, probably via
CC altering host macrophage cytokine profiling and attenuating the cell
CC apoptosis (PubMed:27987050). Could be required for host endothelial-
CC cell invasion (PubMed:16586367). {ECO:0000269|PubMed:16586367,
CC ECO:0000269|PubMed:27987050}.
CC -!- FUNCTION: Expression in Mycobacterium smegmatis, a nonpathogenic
CC species naturally deficient in PE_PGRS genes, results in alteration of
CC the production of host cytokines, including IL-6, IL-1beta, IL-10 and
CC IL-12p40, as well as enhanced survival within macrophages largely via
CC attenuating the apoptosis of macrophages.
CC {ECO:0000269|PubMed:27987050}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:27987050}. Note=Cell envelope-associated protein.
CC {ECO:0000269|PubMed:27987050}.
CC -!- INDUCTION: Highly up-regulated during the early stages of invasion of
CC the human blood-brain barrier. {ECO:0000269|PubMed:16586367}.
CC -!- DISRUPTION PHENOTYPE: Invasion of the infant human brain microvascular
CC endothelial-cell monolayer is significantly decreased in transposon
CC mutant. {ECO:0000269|PubMed:16586367}.
CC -!- SIMILARITY: Belongs to the mycobacterial PE family. PGRS subfamily.
CC {ECO:0000305}.
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DR EMBL; CP009480; AIR13715.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP43730.1; -; Genomic_DNA.
DR RefSeq; WP_010886100.1; NZ_NVQJ01000018.1.
DR RefSeq; YP_177775.1; NC_000962.3.
DR AlphaFoldDB; Q79FU0; -.
DR SMR; Q79FU0; -.
DR STRING; 83332.Rv0980c; -.
DR PaxDb; Q79FU0; -.
DR GeneID; 885327; -.
DR KEGG; mtu:Rv0980c; -.
DR PATRIC; fig|83332.111.peg.1088; -.
DR TubercuList; Rv0980c; -.
DR eggNOG; COG3391; Bacteria.
DR HOGENOM; CLU_000167_16_4_11; -.
DR OMA; GYEMKGY; -.
DR PhylomeDB; Q79FU0; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR011045; N2O_reductase_N.
DR InterPro; IPR001258; NHL_repeat.
DR InterPro; IPR000084; PE-PGRS_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR011964; YVTN_b-propeller_repeat.
DR Pfam; PF01436; NHL; 1.
DR Pfam; PF00934; PE; 1.
DR SUPFAM; SSF50974; SSF50974; 1.
DR TIGRFAMs; TIGR02276; beta_rpt_yvtn; 3.
DR PROSITE; PS51125; NHL; 4.
PE 2: Evidence at transcript level;
KW Cell wall; Reference proteome; Repeat; Secreted; Virulence.
FT CHAIN 1..457
FT /note="PE-PGRS family protein PE_PGRS18"
FT /id="PRO_5008177030"
FT DOMAIN 1..92
FT /note="PE"
FT /evidence="ECO:0000255"
FT REPEAT 291..321
FT /note="NHL 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00504"
FT REPEAT 333..363
FT /note="NHL 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00504"
FT REPEAT 379..404
FT /note="NHL 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00504"
FT REPEAT 419..447
FT /note="NHL 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00504"
SQ SEQUENCE 457 AA; 42075 MW; D06F9D994C87AF8B CRC64;
MSFVNVAPQL VSTAAADAAR IGSAINTANT AAAATTQVLA AAHDEVSTAI AALFGSHGQH
YQAISAQVAA YQERFVLALS QASSTYAVAE AASATPLQNV LDAINAPVQS LTGRPLIGDG
ANGIDGTGQA GGNGGWLWGN GGNGGSGAPG QAGGAGGAAG LIGNGGAGGA GGQGLPFEAG
ANGGAGGAGG WLFGNGGAGG VGGAGGAGTT FGVAGGDGGT GGVGGHGGLI GVGGHGGDGG
TGGTGGAVSL ARAGTAGGAG GGPAGGIGGA GGVGGAGGAA GAVTTITHAS FNDPHGVAVN
PGGNIYVTNQ GSNTVSVIDP VTNTVTGSIT DGNGPSGVAV SPVTGLVFVT NFDSNTVSVI
DPNTNTVTGS IPVGTGAYGV AVNPGGNIYV TNQFSNTVSV IDPATNTVTG SPIPVGLDPT
GVAVNPVTGV VYVTNSLDDT VSVITGEPAR SVCSAAI
