PG2IP_MOUSE
ID PG2IP_MOUSE Reviewed; 699 AA.
AC Q91YL7; Q8CBQ1;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=PGAP2-interacting protein;
DE AltName: Full=Cell wall biogenesis protein 43 C-terminal homolog;
GN Name=Cwh43; Synonyms=Pgap2ip;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Head, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH PGAP2/FRAG1, AND FUNCTION.
RX PubMed=17761529; DOI=10.1091/mbc.e07-05-0482;
RA Umemura M., Fujita M., Yoko-O T., Fukamizu A., Jigami Y.;
RT "Saccharomyces cerevisiae CWH43 is involved in the remodeling of the lipid
RT moiety of GPI anchors to ceramides.";
RL Mol. Biol. Cell 18:4304-4316(2007).
CC -!- FUNCTION: Involved in lipid remodeling during GPI-anchor maturation.
CC {ECO:0000269|PubMed:17761529}.
CC -!- SUBUNIT: Interacts with PGAP2/FRAG1. {ECO:0000269|PubMed:17761529}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q91YL7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91YL7-2; Sequence=VSP_031684;
CC -!- SIMILARITY: Belongs to the PGAP2IP family. {ECO:0000305}.
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DR EMBL; AK035550; BAC29101.1; -; mRNA.
DR EMBL; AK081976; BAC38385.1; -; mRNA.
DR EMBL; BC016523; AAH16523.1; -; mRNA.
DR CCDS; CCDS19341.1; -. [Q91YL7-1]
DR RefSeq; NP_851840.1; NM_181323.2. [Q91YL7-1]
DR AlphaFoldDB; Q91YL7; -.
DR SMR; Q91YL7; -.
DR IntAct; Q91YL7; 1.
DR STRING; 10090.ENSMUSP00000031040; -.
DR GlyGen; Q91YL7; 1 site.
DR PhosphoSitePlus; Q91YL7; -.
DR MaxQB; Q91YL7; -.
DR PaxDb; Q91YL7; -.
DR PRIDE; Q91YL7; -.
DR ProteomicsDB; 288178; -. [Q91YL7-1]
DR ProteomicsDB; 288179; -. [Q91YL7-2]
DR Antibodypedia; 56515; 53 antibodies from 9 providers.
DR DNASU; 231293; -.
DR Ensembl; ENSMUST00000031040; ENSMUSP00000031040; ENSMUSG00000029154. [Q91YL7-1]
DR Ensembl; ENSMUST00000065543; ENSMUSP00000069563; ENSMUSG00000029154. [Q91YL7-2]
DR GeneID; 231293; -.
DR KEGG; mmu:231293; -.
DR UCSC; uc008xsx.1; mouse. [Q91YL7-1]
DR UCSC; uc012dxc.1; mouse. [Q91YL7-2]
DR CTD; 80157; -.
DR MGI; MGI:2444131; Cwh43.
DR VEuPathDB; HostDB:ENSMUSG00000029154; -.
DR eggNOG; ENOG502QVQN; Eukaryota.
DR GeneTree; ENSGT00510000048509; -.
DR HOGENOM; CLU_009808_1_0_1; -.
DR InParanoid; Q91YL7; -.
DR OMA; ITAGIWT; -.
DR OrthoDB; 581809at2759; -.
DR PhylomeDB; Q91YL7; -.
DR TreeFam; TF328857; -.
DR BioGRID-ORCS; 231293; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Cwh43; mouse.
DR PRO; PR:Q91YL7; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q91YL7; protein.
DR Bgee; ENSMUSG00000029154; Expressed in pyloric antrum and 65 other tissues.
DR Genevisible; Q91YL7; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.60.10.10; -; 1.
DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR InterPro; IPR027317; PGAP2IP.
DR PANTHER; PTHR14859:SF1; PTHR14859:SF1; 1.
DR SUPFAM; SSF56219; SSF56219; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; GPI-anchor biosynthesis; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..699
FT /note="PGAP2-interacting protein"
FT /id="PRO_0000320616"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 607..627
FT /note="Required for function in lipid remodeling"
FT /evidence="ECO:0000250"
FT CARBOHYD 536
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 268..353
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_031684"
SQ SEQUENCE 699 AA; 78180 MW; 4F41975D6570D5F8 CRC64;
MPGLWRAIAL ETLLGYVSWS LYHGLSPMIY YFPLQTLELT GLEFFCVAFL SPILLTIPPL
WKLVNKKWTL SLLRIVTVGS IASFEAPNAK LRLMVLALGV SSSLIVQTVT WWSGSGLQRY
LKIWGFILGH VLLLVLRIWY TSLNPIWSYQ MSNRVILTLS AVAVLDRIGT DGDYRNPEGK
KPREVATGRT SLSSWLLPGA AFGSLLFLTH WIFGEVSIVS RWAVSGHPHP GPDPNPFGGA
VLLGFSSGLM LSGSSWLHDA GLAWWMTGAA SAMGLLYLRT WAAAVSGCVL AVFTGSMWPQ
VLGHLVNSGK NSGEAMATGM ILYVLQTFFC AWCTAFKFVP GGVYARERSD VLLGTIMVII
GLSMLFGPKK NLDFLLQTKN SPKTLLRCSE KYMKLILWLF VGVGLLGLGL RHRTYERQLG
RGAPATVVSA AIWPFRFGYD NEGWPNLERS AQLLKETGAD FITILESDAS KPYIGNNDLT
MWLGEKLGFY TDFGPSTRDH TWGIMVLSRY PIVRSEHHLL PSPEGEIAPA ITMTVNVSNR
LVDFVVTHFG NHEDDLDRKL QAIAVSKLLK NCSNQVIFLG YITSEPGSRD YIQLTKHGNV
KDIDSSDGDR WCEYIMYRGL IRLGYARISH AELSDSEIQM AKFRIPDDPA NYRDNQKVVI
DHRGVPKNIH FNPRFGSYKE GHNYENTHHF HMNTPKYFV
