PG30_MYCTU
ID PG30_MYCTU Reviewed; 1011 AA.
AC Q79FL8; I6X1W4; L0T7B2;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=PE-PGRS family protein PE_PGRS30 {ECO:0000305};
GN Name=PE_PGRS30 {ECO:0000312|EMBL:CCP44416.1};
GN OrderedLocusNames=Rv1651c {ECO:0000312|EMBL:CCP44416.1};
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP SUBCELLULAR LOCATION.
RX PubMed=21732963; DOI=10.1111/j.1574-6968.2011.02354.x;
RA Chatrath S., Gupta V.K., Dixit A., Garg L.C.;
RT "The Rv1651c-encoded PE-PGRS30 protein expressed in Mycobacterium smegmatis
RT exhibits polar localization and modulates its growth profile.";
RL FEMS Microbiol. Lett. 322:194-199(2011).
RN [3]
RP FUNCTION IN VIRULENCE, DISRUPTION PHENOTYPE, DOMAIN, AND EXPRESSION IN
RP M.SMEGMATIS.
RC STRAIN=H37Rv;
RX PubMed=22050772; DOI=10.1111/j.1462-5822.2011.01721.x;
RA Iantomasi R., Sali M., Cascioferro A., Palucci I., Zumbo A., Soldini S.,
RA Rocca S., Greco E., Maulucci G., De Spirito M., Fraziano M., Fadda G.,
RA Manganelli R., Delogu G.;
RT "PE_PGRS30 is required for the full virulence of Mycobacterium
RT tuberculosis.";
RL Cell. Microbiol. 14:356-367(2012).
RN [4]
RP SUBCELLULAR LOCATION, AND DOMAIN.
RX PubMed=24530527; DOI=10.1016/j.febslet.2014.01.059;
RA Chatrath S., Gupta V.K., Garg L.C.;
RT "The PGRS domain is responsible for translocation of PE_PGRS30 to cell
RT poles while the PE and the C-terminal domains localize it to the cell
RT wall.";
RL FEBS Lett. 588:990-994(2014).
RN [5]
RP SUBCELLULAR LOCATION, AND DOMAIN.
RX PubMed=25390359; DOI=10.1371/journal.pone.0112482;
RA De Maio F., Maulucci G., Minerva M., Anoosheh S., Palucci I., Iantomasi R.,
RA Palmieri V., Camassa S., Sali M., Sanguinetti M., Bitter W., Manganelli R.,
RA De Spirito M., Delogu G.;
RT "Impact of protein domains on PE_PGRS30 polar localization in
RT Mycobacteria.";
RL PLoS ONE 9:E112482-E112482(2014).
RN [6]
RP FUNCTION, DOMAIN, AND EXPRESSION IN M.SMEGMATIS.
RX PubMed=27129781; DOI=10.1016/j.micinf.2016.04.004;
RA Chatrath S., Gupta V.K., Dixit A., Garg L.C.;
RT "PE_PGRS30 of Mycobacterium tuberculosis mediates suppression of
RT proinflammatory immune response in macrophages through its PGRS and PE
RT domains.";
RL Microbes Infect. 18:536-542(2016).
CC -!- FUNCTION: Mediates suppression of pro-inflammatory immune response in
CC macrophages via modulation of host cytokine response (PubMed:27129781).
CC Required for full virulence. Involved in inhibition of phago-lysosome
CC fusion (PubMed:22050772). {ECO:0000269|PubMed:22050772,
CC ECO:0000269|PubMed:27129781}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:21732963,
CC ECO:0000269|PubMed:24530527, ECO:0000269|PubMed:25390359}. Cell surface
CC {ECO:0000269|PubMed:24530527, ECO:0000269|PubMed:25390359}.
CC Note=Localizes at the cell poles. {ECO:0000269|PubMed:21732963,
CC ECO:0000269|PubMed:24530527, ECO:0000269|PubMed:25390359}.
CC -!- DOMAIN: Both the PE domain and the non-PGRS C-terminal domain possess
CC independent cell wall localization signals. The PGRS domain is
CC responsible for polar localization of PE_PGRS30 (PubMed:24530527,
CC PubMed:25390359). PE and PGRS domains are necessary for the cytokine-
CC modulating function (PubMed:27129781). The non-PGRS C-terminal domain
CC is not exposed on the surface and is not required for full virulence in
CC vivo (PubMed:22050772, PubMed:25390359). {ECO:0000269|PubMed:22050772,
CC ECO:0000269|PubMed:24530527, ECO:0000269|PubMed:25390359,
CC ECO:0000269|PubMed:27129781}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant is attenuated in vivo during
CC murine tuberculosis, specifically during the chronic steps of
CC infection. On day 28 post infection, shows a slight reduction of the
CC bacterial burden. On day 70, shows a strong reduction of the bacterial
CC burden. Mice infected with the mutant show smaller, non-confluent
CC tubercules. Mutant shows impaired ability to survive and multiply
CC within murine macrophages. It is unable to inhibit phago-lysosome
CC fusion in THP-1 cells. {ECO:0000269|PubMed:22050772}.
CC -!- MISCELLANEOUS: Infection of human THP-1 macrophages with M.smegmatis
CC expressing PE_PGRS30 results in reduced production of IL-12, TNF-alpha
CC and IL-6, as compared to infection with vector control M.smegmatis. No
CC differential effects are observed on bacterial persistence inside
CC macrophages or on macrophage mortality upon infection
CC (PubMed:27129781). However, expression of this gene in M.smegmatis is
CC sufficient to enhance the ability to persist intracellularly and induce
CC murine J774 macrophages death (PubMed:22050772). Differential response
CC of the human and murine macrophages could be due to cell-specific
CC processes (Probable). {ECO:0000269|PubMed:22050772,
CC ECO:0000269|PubMed:27129781, ECO:0000305|PubMed:27129781}.
CC -!- SIMILARITY: Belongs to the mycobacterial PE family. PGRS subfamily.
CC {ECO:0000305}.
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DR EMBL; AL123456; CCP44416.1; -; Genomic_DNA.
DR RefSeq; WP_010886125.1; NZ_KK339370.1.
DR RefSeq; YP_177826.1; NC_000962.3.
DR AlphaFoldDB; Q79FL8; -.
DR STRING; 83332.Rv1651c; -.
DR PaxDb; Q79FL8; -.
DR DNASU; 885174; -.
DR GeneID; 885174; -.
DR KEGG; mtu:Rv1651c; -.
DR PATRIC; fig|83332.111.peg.1836; -.
DR TubercuList; Rv1651c; -.
DR eggNOG; COG3391; Bacteria.
DR OMA; IAVGHNS; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR InterPro; IPR000084; PE-PGRS_N.
DR Pfam; PF00934; PE; 1.
PE 1: Evidence at protein level;
KW Cell wall; Reference proteome; Secreted; Virulence.
FT CHAIN 1..1011
FT /note="PE-PGRS family protein PE_PGRS30"
FT /id="PRO_5004287725"
FT DOMAIN 1..93
FT /note="PE"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:25390359"
FT REGION 130..696
FT /note="PGRS domain"
FT /evidence="ECO:0000305|PubMed:25390359"
FT REGION 595..701
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 697..1011
FT /note="C-terminal domain"
FT /evidence="ECO:0000305|PubMed:25390359"
SQ SEQUENCE 1011 AA; 88455 MW; 1F2CBAB67969E066 CRC64;
MSFLLVEPDL VTAAAANLAG IRSALSEAAA AASTPTTALA SAGADEVSAA VSRLFGAYGQ
QFQALNARAA TFHAEFVSLL NGGAAAYTGA EAASVSSMQA LLDAVNAPTQ TLLGRPLIGN
GADGVAGTGS NAGGNGGPGG ILYGNGGNGG AGGNGGAAGL IGNGGAGGAG GAGGAGGAGG
AGGTGGLLYG NGGAGGNGGS AAAAGGAGGN ALLFGNGGNG GSGASGGAAG HAGTIFGNGG
NAGAGSGLAG ADGGLFGNGG DGGSSTSKAG GAGGNALFGN GGDGGSSTVA AGGAGGNTLV
GNGGAGGAGG TSGLTGSGVA GGAGGSVGLW GSGGAGGDGG AATSLLGVGM NAGAGGAGGN
AGLLYGNGGA GGAGGNGGDT TVPLFDSGVG GAGGAGGNAS LFGNGGTGGV GGKGGTSSDL
ASATSGAGGA GGAGGVGGLL YGNGGNGGAG GIGGAAINIL ANAGAGGAGG AAGSSFIGNG
GNGGAGGAGG AAALFSSGVG GAGGSGGTAL LLGSGGAGGN GGTGGANSGS LFASPGGTGG
AGGHGGAGGL IWGNGGAGGN GGNGGTTADG ALEGGTGGIG GTGGSAIAFG NGGQGGAGGT
GGDHSGGNGI GGKGGASGNG GNAGQVFGDG GTGGTGGAGG AGSGTKAGGT GSDGGHGGNA
TLIGNGGDGG AGGAGGAGSP AGAPGNGGTG GTGGVLFGQS GSSGPPGAAA LAFPSLSSSV
PILGPYEDLI ANTVANLASI GNTWLADPAP FLQQYLANQF GYGQLTLTAL TDATRDFAIG
LAGIPPSLQS ALQALAAGDV SGAVTDVLGA VVKVFVSGVD ASDLSNILLL GPVGDLFPIL
SIPGAMSQNF TNVVMTVTDT TIAFSIDTTN LTGVMTFGLP LAMTLNAVGS PITTAIAFAE
STTAFVSAVQ AGNLQAAAAA LVGAPANVAN GFLNGEARLP LALPTSATGG IPVTVEVPVG
GILAPLQPFQ ATAVIPVIGP VTVTLEGTPA GGIVPALVNY APTQLAQAIA P
