ID   PG33_MYCTO              Reviewed;         471 AA.
AC   P9WIF4; L0TAP6; Q50615;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=PE-PGRS family protein PE_PGRS33 {ECO:0000305};
GN   Name=PE_PGRS33; OrderedLocusNames=MT1866;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Induces TNF-alpha release through human Toll-like receptor 2
CC       (TLR2) signaling pathway, leading to macrophage apoptosis.
CC       {ECO:0000250|UniProtKB:P9WIF5}.
CC   -!- SUBUNIT: Interacts with human TLR2. {ECO:0000250|UniProtKB:P9WIF5}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000250|UniProtKB:P9WIF5}. Cell surface
CC       {ECO:0000250|UniProtKB:P9WIF5}. Cell outer membrane
CC       {ECO:0000250|UniProtKB:P9WIF5}. Note=Exported to the cell surface via
CC       the ESX-5 / type VII secretion system (T7SS).
CC       {ECO:0000250|UniProtKB:P9WIF5}.
CC   -!- DOMAIN: Contains an N-terminal PE domain, followed by a conserved
CC       linker region and a C-terminal PGRS domain.
CC       {ECO:0000250|UniProtKB:P9WIF5}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PE family. PGRS subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK46139.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK46139.1; ALT_INIT; Genomic_DNA.
DR   PIR; C70720; C70720.
DR   RefSeq; WP_031676687.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WIF4; -.
DR   EnsemblBacteria; AAK46139; AAK46139; MT1866.
DR   KEGG; mtc:MT1866; -.
DR   PATRIC; fig|83331.31.peg.2009; -.
DR   HOGENOM; CLU_000167_16_10_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   InterPro; IPR000084; PE-PGRS_N.
DR   Pfam; PF00934; PE; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Cell wall; Membrane; Secreted; Virulence.
FT   CHAIN           1..471
FT                   /note="PE-PGRS family protein PE_PGRS33"
FT                   /id="PRO_0000428016"
FT   DOMAIN          1..93
FT                   /note="PE"
FT                   /evidence="ECO:0000255"
FT   REGION          1..30
FT                   /note="Essential for translocation to the cell surface"
FT                   /evidence="ECO:0000250|UniProtKB:P9WIF5"
FT   REGION          140..230
FT                   /note="Interacts with TLR2"
FT                   /evidence="ECO:0000250|UniProtKB:P9WIF5"
SQ   SEQUENCE   471 AA;  38729 MW;  BD3A8C736A9CEDB8 CRC64;
     MSFVVTIPEA LAAVATDLAG IGSTIGTANA AAAVPTTTVL AAAADEVSAA MAALFSGHAQ
     AYQALSAQAA LFHEQFVRAL TAGAGSYAAA EAASAAPLEG VLDVINAPAL ALLGRPLIGN
     GANGAPGTGA NGGDGGILIG NGGAGGSGAA GMPGGNGGAA GLFGNGGAGG AGGNVASGTA
     GFGGAGGAGG NGGLLFGAGG AGGVGGLAAD AGDGGAGGDG GLFFGVGGAG GAGGTGTNVT
     GGAGGAGGNG GLLFGAGGVG GVGGDGVAFL GTAPGGPGGA GGAGGLFGVG GAGGAGGIGL
     VGNGGAGGSG GSALLWGDGG AGGAGGVGST TGGAGGAGGN AGLLVGAGGA GGAGALGGGA
     TGVGGAGGNG GTAGLLFGAG GAGGAGGFGF GGAGGAGGLG GKAGLIGDGG DGGAGGNGTG
     AKGGDGGAGG GAILVGNGGN GGNAGSGTPN GSAGTGGAGG LLGKNGMNGL P