ID   PG62_MYCTU              Reviewed;         504 AA.
AC   L7N680; I6Y4K8;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2013, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=PE-PGRS family protein PE_PGRS62 {ECO:0000305};
GN   Name=PE_PGRS62 {ECO:0000312|EMBL:CCP46641.1};
GN   OrderedLocusNames=Rv3812 {ECO:0000312|EMBL:CCP46641.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   BIOTECHNOLOGY.
RC   STRAIN=H37Rv;
RX   PubMed=18719176; DOI=10.1099/jmm.0.47565-0;
RA   Chaitra M.G., Shaila M.S., Nayak R.;
RT   "Characterization of T-cell immunogenicity of two PE/PPE proteins of
RT   Mycobacterium tuberculosis.";
RL   J. Med. Microbiol. 57:1079-1086(2008).
RN   [3]
RP   FUNCTION, EXPRESSION IN M.SMEGMATIS, AND SUBCELLULAR LOCATION.
RX   PubMed=23167250; DOI=10.1111/cmi.12073;
RA   Thi E.P., Hong C.J., Sanghera G., Reiner N.E.;
RT   "Identification of the Mycobacterium tuberculosis protein PE-PGRS62 as a
RT   novel effector that functions to block phagosome maturation and inhibit
RT   iNOS expression.";
RL   Cell. Microbiol. 15:795-808(2013).
RN   [4]
RP   INTERACTION WITH HOST TLR2, AND BIOTECHNOLOGY.
RX   PubMed=23788727; DOI=10.1093/infdis/jit281;
RA   Vani J., Shaila M.S., Trinath J., Balaji K.N., Kaveri S.V., Bayry J.;
RT   "Mycobacterium tuberculosis cell wall-associated Rv3812 protein induces
RT   strong dendritic cell-mediated interferon gamma responses and exhibits
RT   vaccine potential.";
RL   J. Infect. Dis. 208:1034-1036(2013).
RN   [5]
RP   FUNCTION, AND EXPRESSION IN M.SMEGMATIS.
RC   STRAIN=H37Rv;
RX   PubMed=30937925; DOI=10.1002/jcp.28577;
RA   Long Q., Xiang X., Yin Q., Li S., Yang W., Sun H., Liu Q., Xie J., Deng W.;
RT   "PE_PGRS62 promotes the survival of Mycobacterium smegmatis within
RT   macrophages via disrupting ER stress-mediated apoptosis.";
RL   J. Cell. Physiol. 234:19774-19784(2019).
CC   -!- FUNCTION: Supports mycobacterial virulence via inhibition of phagosome
CC       maturation and host inducible nitric oxide synthase (iNOS) expression
CC       (PubMed:23167250). May promote the survival within macrophages by
CC       disturbing the cytokines profiles and blocking the endoplasmic
CC       reticulum (ER) stress-mediated apoptosis (PubMed:30937925). May also
CC       affect bacterial cell wall composition (PubMed:23167250).
CC       {ECO:0000269|PubMed:23167250, ECO:0000269|PubMed:30937925}.
CC   -!- FUNCTION: Expression in Mycobacterium smegmatis, a nonpathogenic
CC       species naturally deficient in PE_PGRS genes, results in enhanced
CC       resistance to various in vitro stresses (PubMed:30937925). It also
CC       leads to phagosome maturation arrest and increased survival in
CC       macrophages (PubMed:30937925, PubMed:23167250).
CC       {ECO:0000269|PubMed:23167250, ECO:0000269|PubMed:30937925}.
CC   -!- SUBUNIT: Interacts with host Toll-like receptor 2 (TLR2).
CC       {ECO:0000269|PubMed:23788727}.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC       {ECO:0000269|PubMed:23167250}.
CC   -!- BIOTECHNOLOGY: Exhibits vaccine potential (PubMed:18719176,
CC       PubMed:23788727). Immunogenicity is demonstrated in BALB/c mice
CC       (PubMed:18719176). Interaction with TLR2 induces activation of human
CC       dendritic cells (DCs) (PubMed:23788727). Immunization with this protein
CC       induces a significant number of CD8+ T cells and a strong Th1-type
CC       response, with high gamma interferon (IFN-gamma) and low interleukin-4
CC       responses, and activation of the bactericidal activity of macrophages.
CC       Identified T-cell epitopes may contribute to immunity against
CC       tuberculosis if included in a vaccine (PubMed:18719176).
CC       {ECO:0000269|PubMed:18719176, ECO:0000269|PubMed:23788727}.
CC   -!- SIMILARITY: Belongs to the mycobacterial PE family. PGRS subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP46641.1; -; Genomic_DNA.
DR   RefSeq; WP_003420809.1; NZ_NVQJ01000022.1.
DR   RefSeq; YP_178019.1; NC_000962.3.
DR   AlphaFoldDB; L7N680; -.
DR   STRING; 83332.Rv3812; -.
DR   PaxDb; L7N680; -.
DR   PRIDE; L7N680; -.
DR   DNASU; 886143; -.
DR   GeneID; 886143; -.
DR   KEGG; mtu:Rv3812; -.
DR   PATRIC; fig|83332.111.peg.4237; -.
DR   TubercuList; Rv3812; -.
DR   eggNOG; COG0657; Bacteria.
DR   OMA; MNDFHTI; -.
DR   PhylomeDB; L7N680; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   InterPro; IPR000084; PE-PGRS_N.
DR   Pfam; PF00934; PE; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Reference proteome; Secreted; Virulence.
FT   CHAIN           1..504
FT                   /note="PE-PGRS family protein PE_PGRS62"
FT                   /id="PRO_0000451249"
FT   DOMAIN          4..94
FT                   /note="PE"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   504 AA;  51821 MW;  F1EB182132322BCF CRC64;
     MSFVVTVPEA VAAAAGDLAA IGSTLREATA AAAGPTTGLA AAAADDVSIA VSQLFGRYGQ
     EFQTVSNQLA AFHTEFVRTL NRGAAAYLNT ESANGGQLFG QIEAGQRAVS AAAAAAPGGA
     YGQLVANTAT NLESLYGAWS ANPFPFLRQI IANQQVYWQQ IAAALANAVQ NFPALVANLP
     AAIDAAVQQF LAFNAAYYIQ QIISSQIGFA QLFATTVGQG VTSVIAGWPN LAAELQLAFQ
     QLLVGDYNAA VANLGKAMTN LLVTGFDTSD VTIGTMGTTI SVTAKPKLLG PLGDLFTIMT
     IPAQEAQYFT NLMPPSILRD MSQNFTNVLT TLSNPNIQAV ASFDIATTAG TLSTFFGVPL
     VLTYATLGAP FASLNAIATS AETIEQALLA GNYLGAVGAL IDAPAHALDG FLNSATVLDT
     PILVPTGLPS PLPPTVGITL HLPFDGILVP PHPVTATISF PGAPVPIPGF PTTVTVFGTP
     FMGMAPLLIN YIPQQLALAI KPAA