PGA14_CANAL
ID PGA14_CANAL Reviewed; 131 AA.
AC Q5A4X8; A0A1D8PMW1;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Hydrophilin PGA14;
DE AltName: Full=Predicted GPI-anchored protein 14;
DE Flags: Precursor;
GN Name=PGA14; OrderedLocusNames=CAALFM_C500270WA;
GN ORFNames=CaO19.8583, CaO19.968;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12734798; DOI=10.1002/yea.988;
RA Lee S.A., Wormsley S., Kamoun S., Lee A.F., Joiner K., Wong B.;
RT "An analysis of the Candida albicans genome database for soluble secreted
RT proteins using computer-based prediction algorithms.";
RL Yeast 20:595-610(2003).
RN [5]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [6]
RP INDUCTION.
RX PubMed=15814841; DOI=10.1091/mbc.e05-01-0071;
RA Garcia-Sanchez S., Mavor A.L., Russell C.L., Argimon S., Dennison P.,
RA Enjalbert B., Brown A.J.;
RT "Global roles of Ssn6 in Tup1- and Nrg1-dependent gene regulation in the
RT fungal pathogen, Candida albicans.";
RL Mol. Biol. Cell 16:2913-2925(2005).
RN [7]
RP INDUCTION.
RX PubMed=16455273; DOI=10.1016/j.fgb.2005.12.002;
RA Castillo L., Martinez A.I., Garcera A., Garcia-Martinez J.,
RA Ruiz-Herrera J., Valentin E., Sentandreu R.;
RT "Genomic response programs of Candida albicans following protoplasting and
RT regeneration.";
RL Fungal Genet. Biol. 43:124-134(2006).
RN [8]
RP INDUCTION.
RX PubMed=23208712; DOI=10.1128/aac.02040-12;
RA Silva L.V., Sanguinetti M., Vandeputte P., Torelli R., Rochat B.,
RA Sanglard D.;
RT "Milbemycins: more than efflux inhibitors for fungal pathogens.";
RL Antimicrob. Agents Chemother. 57:873-886(2013).
CC -!- FUNCTION: Hydrophilin which is essential to overcome the simple stress
CC of the desiccation-rehydration process. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane {ECO:0000305};
CC Lipid-anchor, GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Induced during cell wall regeneration and upon milbemycins
CC A3 oxim derivative (A3Ox) treatment. Expression is also regulated by
CC SSN6. {ECO:0000269|PubMed:15814841, ECO:0000269|PubMed:16455273,
CC ECO:0000269|PubMed:23208712}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PGA14 family. {ECO:0000305}.
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DR EMBL; CP017627; AOW29473.1; -; Genomic_DNA.
DR RefSeq; XP_716806.1; XM_711713.1.
DR AlphaFoldDB; Q5A4X8; -.
DR STRING; 237561.Q5A4X8; -.
DR GeneID; 3641560; -.
DR KEGG; cal:CAALFM_C500270WA; -.
DR CGD; CAL0000177457; PGA14.
DR VEuPathDB; FungiDB:C5_00270W_A; -.
DR eggNOG; ENOG502RZ9P; Eukaryota.
DR HOGENOM; CLU_098355_0_0_1; -.
DR InParanoid; Q5A4X8; -.
DR OMA; GTHRYGK; -.
DR OrthoDB; 1646229at2759; -.
DR PRO; PR:Q5A4X8; -.
DR Proteomes; UP000000559; Chromosome 5.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal; Stress response.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..105
FT /note="Hydrophilin PGA14"
FT /id="PRO_0000424802"
FT PROPEP 106..131
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424803"
FT REGION 42..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..63
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 105
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 131 AA; 13744 MW; BF1CB7945A1BD9B6 CRC64;
MKFTTVATVF AISSLAAAKG GEKDHGKAST VTKYVTETTH RYGRFDKTSR SKKPKETGTH
RYGKFNKTPR PVTTTVLVKE SDLPKKRDAV VARDSKNASS NSTTSSGNNG VATGVSLGLA
GVLAVGAALV I
