PGA1_SCHPO
ID PGA1_SCHPO Reviewed; 178 AA.
AC C6Y4B3;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 2.
DT 25-MAY-2022, entry version 57.
DE RecName: Full=GPI mannosyltransferase 2 subunit C167.09;
DE Flags: Precursor;
GN ORFNames=SPAC167.09;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP IDENTIFICATION.
RX PubMed=18488015; DOI=10.1038/nature07002;
RA Wilhelm B.T., Marguerat S., Watt S., Schubert F., Wood V., Goodhead I.,
RA Penkett C.J., Rogers J., Baehler J.;
RT "Dynamic repertoire of a eukaryotic transcriptome surveyed at single-
RT nucleotide resolution.";
RL Nature 453:1239-1243(2008).
CC -!- FUNCTION: Essential component of the GPI mannosyltransferase 2 complex.
CC Responsible for the transfer of the second mannose to the
CC glycosylphosphatidylinositol during GPI precursor assembly (By
CC similarity). {ECO:0000250}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Part of the GPI mannosyltransferase 2 complex composed of
CC gpi18 and C167.09. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
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DR EMBL; CU329670; CBA11495.2; -; Genomic_DNA.
DR RefSeq; XP_002742504.2; XM_002742458.2.
DR AlphaFoldDB; C6Y4B3; -.
DR STRING; 4896.SPAC167.09.1; -.
DR MaxQB; C6Y4B3; -.
DR PaxDb; C6Y4B3; -.
DR EnsemblFungi; SPAC167.09.1; SPAC167.09.1:pep; SPAC167.09.
DR PomBase; SPAC167.09; -.
DR VEuPathDB; FungiDB:SPAC167.09; -.
DR HOGENOM; CLU_1518719_0_0_1; -.
DR InParanoid; C6Y4B3; -.
DR OMA; YQLRASW; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:C6Y4B3; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0120097; C:glycosylphosphatidylinositol-mannosyltransferase II complex; ISO:PomBase.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; ISO:PomBase.
DR GO; GO:0031501; C:mannosyltransferase complex; IBA:GO_Central.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; ISO:PomBase.
DR GO; GO:0097502; P:mannosylation; IEA:GOC.
DR InterPro; IPR019433; GPI_ManTrfase_II_coact_Pga1.
DR PANTHER; PTHR28022; PTHR28022; 1.
DR Pfam; PF10333; Pga1; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Glycosyltransferase;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Signal; Transferase;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..178
FT /note="GPI mannosyltransferase 2 subunit C167.09"
FT /id="PRO_0000389147"
FT TOPO_DOM 21..152
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CARBOHYD 48
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 178 AA; 19995 MW; 4F7AC9F6028917CF CRC64;
MREFRLIFVL LFFLPSFAIA NTEIINVETG DSNKLLTSNS VCDIELSNNS SIPLLLKPNP
TIPQAAGAPQ QSKYTFSVCG LKPLQKYQIR ASWPAVYPSD IILNYNITHI IVEINASFYS
HNESLMKRPP LVPLRLVVEP LLLGFLPKSV LPIVGFVFVI ILIALICMTN LFIKHKRD
