PGA1_YEAST
ID PGA1_YEAST Reviewed; 198 AA.
AC P53896; D6W125;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=GPI mannosyltransferase 2 subunit PGA1;
DE AltName: Full=Processing of GAS1 and ALP protein 1;
DE Flags: Precursor;
GN Name=PGA1; OrderedLocusNames=YNL158W; ORFNames=N1739;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8686380;
RX DOI=10.1002/(sici)1097-0061(199602)12:2<169::aid-yea894>3.0.co;2-b;
RA Nasr F., Becam A.-M., Herbert C.J.;
RT "The sequence of 36.8 kb from the left arm of chromosome XIV reveals 24
RT complete open reading frames: 18 correspond to new genes, one of which
RT encodes a protein similar to the human myotonic dystrophy kinase.";
RL Yeast 12:169-175(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP FUNCTION, INTERACTION WITH GPI18, SUBCELLULAR LOCATION, AND GLYCOSYLATION.
RX PubMed=17615295; DOI=10.1091/mbc.e07-03-0258;
RA Sato K., Noda Y., Yoda K.;
RT "Pga1 is an essential component of glycosylphosphatidylinositol-
RT mannosyltransferase II of Saccharomyces cerevisiae.";
RL Mol. Biol. Cell 18:3472-3485(2007).
CC -!- FUNCTION: Essential component of the GPI mannosyltransferase 2 complex.
CC Responsible for the transfer of the second mannose to the
CC glycosylphosphatidylinositol during GPI precursor assembly.
CC {ECO:0000269|PubMed:17615295}.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis.
CC -!- SUBUNIT: Part of the GPI mannosyltransferase 2 complex composed of
CC GPI18 and PGA1.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:17615295}; Single-pass
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:17615295}.
CC -!- MISCELLANEOUS: Present with 6940 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X92517; CAA63281.1; -; Genomic_DNA.
DR EMBL; Z71434; CAA96045.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10391.1; -; Genomic_DNA.
DR PIR; S60969; S60969.
DR RefSeq; NP_014241.1; NM_001182996.1.
DR AlphaFoldDB; P53896; -.
DR BioGRID; 35671; 97.
DR ComplexPortal; CPX-1269; Glycosylphosphatidylinositol-mannosyltransferase II complex.
DR DIP; DIP-4740N; -.
DR IntAct; P53896; 9.
DR STRING; 4932.YNL158W; -.
DR iPTMnet; P53896; -.
DR MaxQB; P53896; -.
DR PaxDb; P53896; -.
DR PRIDE; P53896; -.
DR EnsemblFungi; YNL158W_mRNA; YNL158W; YNL158W.
DR GeneID; 855564; -.
DR KEGG; sce:YNL158W; -.
DR SGD; S000005102; PGA1.
DR VEuPathDB; FungiDB:YNL158W; -.
DR eggNOG; ENOG502S2MY; Eukaryota.
DR HOGENOM; CLU_116423_0_0_1; -.
DR InParanoid; P53896; -.
DR OMA; YQIKICW; -.
DR BioCyc; YEAST:G3O-33174-MON; -.
DR UniPathway; UPA00196; -.
DR PRO; PR:P53896; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53896; protein.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:ComplexPortal.
DR GO; GO:0120097; C:glycosylphosphatidylinositol-mannosyltransferase II complex; IPI:ComplexPortal.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0031501; C:mannosyltransferase complex; IGI:SGD.
DR GO; GO:0005635; C:nuclear envelope; HDA:SGD.
DR GO; GO:0000030; F:mannosyltransferase activity; IMP:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IC:ComplexPortal.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IMP:SGD.
DR GO; GO:0035268; P:protein mannosylation; IC:ComplexPortal.
DR InterPro; IPR019433; GPI_ManTrfase_II_coact_Pga1.
DR PANTHER; PTHR28022; PTHR28022; 1.
DR Pfam; PF10333; Pga1; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; Glycosyltransferase;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Signal; Transferase;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..198
FT /note="GPI mannosyltransferase 2 subunit PGA1"
FT /id="PRO_0000203415"
FT TOPO_DOM 24..163
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..198
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 198 AA; 22383 MW; B54A4AF0A0E6DB5D CRC64;
MVRPQNVHWF IATIVFFIGF VHANTESILY KVPHNFPLKK PRDSSTYARD VNLISSISLS
GEAMSQITIE ANTTDLELHN TTYIELADLQ RDETYQIKVC WSAIHPISIN NLQTITIPRF
TEFQGTKSDY ARILVTFQVL SDSYPSEHAM VPIQVSLITT RLGIPVDIYP TLIVMVLLVA
GLVVTRAPHV LNDLLLKF
