PGA30_CANAL
ID PGA30_CANAL Reviewed; 277 AA.
AC Q5A5U6; A0A1D8PLZ1;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Cell wall protein PGA30;
DE AltName: Full=GPI-anchored protein 30;
DE AltName: Full=LDG family protein 6;
DE Flags: Precursor;
GN Name=PGA30; Synonyms=LDG6; OrderedLocusNames=CAALFM_C404070CA;
GN ORFNames=CaO19.12763, CaO19.5303;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=18712765; DOI=10.1002/pmic.200800110;
RA Castillo L., Calvo E., Martinez A.I., Ruiz-Herrera J., Valentin E.,
RA Lopez J.A., Sentandreu R.;
RT "A study of the Candida albicans cell wall proteome.";
RL Proteomics 8:3871-3881(2008).
RN [6]
RP INDUCTION.
RX PubMed=23208712; DOI=10.1128/aac.02040-12;
RA Silva L.V., Sanguinetti M., Vandeputte P., Torelli R., Rochat B.,
RA Sanglard D.;
RT "Milbemycins: more than efflux inhibitors for fungal pathogens.";
RL Antimicrob. Agents Chemother. 57:873-886(2013).
CC -!- FUNCTION: Component of the cell wall involved in virulence which plays
CC a role in the relationship between C.albicans and the host.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:18712765}. Membrane {ECO:0000269|PubMed:18712765};
CC Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:18712765}.
CC -!- INDUCTION: Up-regulated upon milbemycin A3 oxim derivative (A3Ox)
CC treatment. {ECO:0000269|PubMed:23208712}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
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DR EMBL; CP017626; AOW29156.1; -; Genomic_DNA.
DR RefSeq; XP_717106.1; XM_712013.1.
DR AlphaFoldDB; Q5A5U6; -.
DR STRING; 237561.Q5A5U6; -.
DR GeneID; 3641227; -.
DR KEGG; cal:CAALFM_C404070CA; -.
DR CGD; CAL0000200830; PGA30.
DR VEuPathDB; FungiDB:C4_04070C_A; -.
DR eggNOG; ENOG502SXWZ; Eukaryota.
DR HOGENOM; CLU_083354_0_0_1; -.
DR InParanoid; Q5A5U6; -.
DR OMA; TITEKCV; -.
DR OrthoDB; 1611342at2759; -.
DR PRO; PR:Q5A5U6; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009986; C:cell surface; IDA:CGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0030446; C:hyphal cell wall; IDA:CGD.
DR GO; GO:0030445; C:yeast-form cell wall; IDA:CGD.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..253
FT /note="Cell wall protein PGA30"
FT /id="PRO_0000424727"
FT PROPEP 254..277
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424728"
FT REGION 219..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 253
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 129
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 277 AA; 28389 MW; 9E084BDAC1316C83 CRC64;
MKYFTIATVL TLASSALAAI RDVQLFAQSS NEEINNLGLI SRREGAGVNY LFLASGAETL
KFDDETFTIF SELQTGSTTA RQSLVVSGGV VQLSVSGQPL HVEIAEDGSV KFAGSDSVAA
AKNINDPYNY SKDSFAVVTN GGEGSIPFKI IAKFIGGGKS SSVTKHTEAP TTSPVYSNKT
VTVFTTYCPE STTITLTICS EVCTPTVIET SGSVTVSSVL PSSSTEAPPK TSVAAPSTTA
EAQTTAPVTS YEGGANEIVG GGSMAIALAA AAIGLVI
